Description
The cells have a finite lifespan of about 25 serial passages from the tissue of origin; they can be transformed by SV40; growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the mediumTumorigenecity:Isoenzyme:G6PD, BHistopathology:normalSubculture:Split ratio:Media change:Reverse transcritase:negativeProduction