Description
Mononuclear cells (MNCs) are separated from human peripheral blood by either a density gradient centrifugation protocol or by apheresis. The mononuclear cells are composed of lymphocytes (T cells, B cells and NK cells), monocytes, dendritic cells and stem/progenitor cells. These cells are critical components of the immune system and play key roles in the humoral and cell-mediated immune responses. Mononuclear cells are commonly used in research and clinical applications in the areas of immunology, infectious disease, hematological malignancies, vaccine development, transplant therapy, and high-throughput screening. StemExpress offers mononuclear cells from peripheral blood obtained from healthy IRB consented donors in an assortment of pre-selected volumes and formats (cryopreserved or fresh). The MNC population can be used for further isolations of specific cell types. Isolated MNCs are characterized by the expression of CD45 and PI using flow cytometry to ensure a =85% viablity.