293T from ATCC

Product Specs
Item293T
CompanyATCC
Price $431.00Supplier Page View Company Product Page
Catalog NumberCRL-3216
TypeCell Biology
SpeciesHomo sapiens
Tissuekidney:Embryo
Cell Line293T
Tissue (standardized)Kidney
Agefetus

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) miR-17-5p inhibits classical swine fever virus replication by targeting the PKD2 -regulated AMPK/mTOR autophagy pathwayVirulenceDecember 31, 2026Xinxian Wang, Shurui Yang, Lichun Xie, Shanshan Qi, Libo Gao, Yongmei Li, Qian Li, Junlong Bi, Jianping Liu, Yongneng Li, et al.and the cell number per well upon cell seeding. The human embryonic kidney cell line HEK-293T (ATCC CRL-3216) was cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, USA) supplemented with 10% fetal bovine

(2) Streptococcus suis Stk1 sensitizes epithelial cells to ferroptosis and exacerbates disruption of the respiratory epithelial barrierEmerging Microbes & InfectionsDecember 31, 2026Lang Tian, Ruicheng Yang, Ting Qi, Wenquan Ouyang, Hongshuo Liu, Dong Huo, Hang Li, Chuyue Zhou, Manman Xu, Haojie Li, et al.(OD600). Porcine tracheal epithelial cells (NPTr) and human embryonic kidney cells (HEK293 T, ATCC CRL-3216) were maintained in Dulbecco’s Modified Eagle Medium (DMEM/High Glucose, Hyclone) supplemented with

(3) Directed evolution of novel AAV capsids for enhanced delivery to mouse and human Schwann cellsMol Ther Nucleic AcidsJune 16, 2026Ana D. Carneiro, Alan M. Nisanov, Hyuncheol Lee, David V. SchafferSchwann cell-associated therapies. Materials and methods Cell culture conditions HEK293T cells (ATCC, CRL-3216) were grown in DMEM (Dow Corning) supplemented with 10% fetal bovine serum (Invitrogen) and 1% Antibiotic-Antimycotic

(4) Influenza B mosaic HA mRNA-LNP vaccines are cross-reactive and protective in miceMolecular Therapy Nucleic AcidsJune 16, 2026Irene González-Domínguez, Marta Boza, Adam Abdeljawad, William Shea, Eduard Puente-Massaguer, Mitali Mishra, Nicholas Lemus, Tsoi Ying Lai, Hiromi Muramatsu, Kieu Lam, et al.Sequences for the mHA antigens were reported previously.25 Cells, viruses, and vaccines HEK 293 T (CRL-3216, ATCC) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% v/v fetal bovine serum (FBS

(5) PRMT5–mediated symmetric dimethylation of SHBs at Arg169 stabilizes SHBs and promotes angiogenesis and tumor growthTumour Virus ResearchJune 1, 2026Shuxiang Wu, Xiaozhen Chen, Yilin Huang, Fangrong Zhang, Xinjian Lin, Xu Linand Huh7 (JCRB, JCRB0403), endothelial cell line EA.hy926 (ATCC, CRL–2922™), and HEK293T cells (ATCC, CRL–3216) were obtained from the American Type Culture Collection (ATCC) and the Japanese Collection of Research

(6) Protocol for enhancing Cas9 efficiency and fidelity through structure-guided phosphate-locking loop engineeringSTAR ProtocolsJune 1, 2026Meiying Yang, Guanqiao Chen, Ji Xiao, Xin Zhang, Zheng Hu, Bo ZhouCell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, HEK293T cells,ATCC,CRL-3216 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(7) Autism-linked protein POSH balances NMDAR signaling via a self-limiting kinase-scaffold loopiScienceMay 15, 2026Minghui Yao, Shuo Wang, Feng Zhang, Lei Shi, Zhiheng Xureplaced with fresh medium every 4 days. Cell line Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were used for heterologous expression and biochemical experiments. Cells were maintained in Dulbecco’s

(8) A bacteriophage-encoded protein gp119 suppresses deoxyinosine repair to protect hypermutated viral genomeiScienceMay 15, 2026Chensi Miao, Weikuan Liu, Xuyang Ren, Yanmin Zhang, Xing Changkanamycin (50 μg/mL), or chloramphenicol (25 μg/mL). Cell line HEK293T cells were purchased from ATCC (CRL-3216) and cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gibco), 1% penicillin/streptomycin

(9) Activation-gated, T cell-restricted silencing of Dapk1 enhances Bacille Calmette-Guérin-elicited protective CD4+ memoryiScienceMay 15, 2026Zixuan Liu, Weihuang Liu, Chenyu Zhao, Xuanchang Bai, Fangting Zhou, Hang Sun, Jiahe Shi, Pinru Chen, Min Liu, Qin Panlines,Experimental models: Cell lines,Experimental models: Cell lines ,, Human: HEK293T,ATCC,Cat# CRL-3216 Jurket cellsMOLT-4,ATCCATCC,Cat# TIB-152Cat# CRL-1582 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, Oligonucleotides

(10) Localized phosphoinositide metabolism regulates STIM1/ORAI1 fast inactivationiScienceMay 15, 2026Ning Dai, Shawn M. Lamothe, Jody Groenendyk, Nicolas Touret, Harley T. Kurata, Marek Michalakoutcomes of this study. Both MEF cells and human embryonic kidney cells (HEK293T; female, ATCC Cat# MSPP-CRL3216) were cultured in accordance with established protocols using DMEM-10 (Dulbecco’s Modified Eagle’s Medium

(11) RadD from Fusobacterium nucleatum Engages NKp46 to Promote Antitumor CytotoxicityeLifeMay 1, 2026Ahmed Rishiq, Johanna Galski, Reem Bsoul, Mingdong Liu, Rema Darawshe, Renate Lux, Gilad Bachrach, Ofer Mandelboimprotein",Ccm-1 fusion protein- Ccm-1 Ig,In-house,N/A, Cell line (Homo-sapiens),HEK293T,In-house,ATCC: CRL-3216,Human embryonic kidney cell lineCell line maintained in Ofer Mandelboim’s lab Cell line (Homo sapiens),MCF7,In-house,ATCC:

(12) Transcriptomic analysis of cells following decreased mitochondrial DNA-copy number reveals compensatory mechanisms in mitochondrial DNA replication and cellular energeticsExperimental Cell ResearchMay 1, 2026Jiaqi Xie, Phyo W. Win, Charles Newcomb, Shaopeng Zeng, Christina A. Castellani, Dan E. Arkingenergy sources. 2 Methods 2.1 Culturing HEK293T cells Human embryonic kidney (HEK) 293T cells (ATCC CRL-3216) were selected for chemical perturbation experiments based on prior work demonstrating that mtDNA depletion

(13) Impaired glycosylation promotes rapid transition to hepatocellular carcinoma in model of diet induced steatotic liver diseaseJournal of Clinical InvestigationMay 1, 2026Abhishek K. Singh, Balkrishna Chaube, Kathryn M. Citrin, Joseph W.M. Fowler, Sungwoon Lee, Jonatas Catarino, James Knight, Sarah C. Lowery, Sonal Shree, Keira E. Mahoney, et al.mM/L glutamine and 10% FBS, along with penicillin/streptomycin (Life Technologies). HEK293T (ATCC, CRL-3216) cells were also part of our study and were cultured in DMEM high glucose supplemented with 10% FBS

(14) Differential plasticity of excitatory and inhibitory reticulospinal fibers after spinal cord injury: Implication for recoveryNeural Regeneration ResearchMay 1, 2026Rozaria Jeleva, Carmen Denecke Muhr, Alina P. Liebisch, Florence M. Bareyrepreviously described protocol (Challis et al., 2019). HEK293T cells (standard cell line to produce rAAVs; crl3216; ATCC, Teddington, UK) were grown in DMEM (high glucose, GlutaMAX, Thermo Fisher Scientific) supplemented

(15) Immortalization of Human Adult Alveolar Epithelial Cells to Study Environmental Exposures of the Distal LungCurr ProtocApril 30, 2026Evelyn Tran, Laurence St. Pierre, Diego Velarde, Bianca Dal Bó, Juan Ramon Alvarez, Kweku Ofosu-Asante, Nazarius S. Lamango, Yong Huang, Crystal N. Marconett, Zea Borok, et al.all appropriate institutional guidelines and safety requirements. Materials HEK 293T cells (ATCC, CRL‐3216, RRID:CVCL_0063)HEK 293T culture medium (see recipe)Sterile 1× DPBS (Corning, 21‐031‐CV)0.05% (w/v)

(16) Reprogramming of bacterial virulence by lysine acetylationNat CommunApril 27, 2026Ole Schmöker, Britta Girbardt, Sabrina Schulze, Gottfried J. Palm, Leona Berndt, Jens Hoppen, Nilüfer Kara, Xenia Schöps, Ruba Al-Abdulla, Klara Garz, et al.fractionation of transfected cells For subcellular fractionation, HEK293T cells originating from ATCC (CRL-3216) were transfected using Ca-phosphate precipitation method. For this, 12 × 106 cells were plated on 150

(17) Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potencyNat CommunApril 24, 2026Diana Ly, Hyewon Jang, Adhiraj Goel, Arnav Singh, Aditya Raguramor Maxi (12963) Kits according to the manufacturer’s protocols. Cell culture HEK293T cells (ATCC; CRL-3216), Gesicle Producer 293T cells (Takara; 632617), HEK293T/17 cells (ATCC; CRL-11268), and Neuro-2a cells

(18) ADAR1 and ADAR2 associate with the RNA exosome and modulate RNA stabilityNucleic Acids ResApril 24, 2026Dragana Vukić, Qiupei Du, Anna Cherian, Damiano Amoruso, Květoslava Brožinová, Ludivine Wacheul, Valentina Lacovich, Christiane Zorbas, Leena Yadav, Jiří Sedmík, et al.mutagenesis procedures are provided in the Supplementary data. Cells and culture conditions HEK293T (ATCC, CRL-3216) cells were maintained in Minimum Essential Medium (MEM) with Earle’s salts (Biosera, #LM-E1141), and

(19) Ferrodoxin 1 (FDX1) drives paclitaxel resistance in ovarian cancer via copper metabolism and ULK1/ATG13-mediated autophagy: overcome by pH/ROS-responsive PPD/PDP@si-FDX1 nanomicellesJ Exp Clin Cancer ResApril 23, 2026Yangmei Gong, Zhizhi Deng, Jie Wu, Yi Hucontaining 5% CO₂, and subcultured once reaching approximately 80–90% confluence. The HEK293T cell line (CRL-3216, ATCC) was cultured in DMEM medium (11965092, Gibco, USA) containing 10% FBS, 10 µg/mL streptomycin,

(20) A microRNA expression signature in infant t(4;11) KMT2A::AFF1+ BCP-ALL uncovers novel therapeutic targetsHemasphereApril 23, 2026Camille Malouf, Alasdair Duguid, Kirsten S. Vrenken, Tom Leah, Ragini Medhi, Giuseppina Camiolo, Leslie Nitsche, Hélène Jakobczyk, Rishi S. Kotecha, Richard A. Anderson, et al.P/S 1% l‐glut. Lentivirus production in 293T cells and transduction experiments 293T cells (ATCC®CRL‐3216™) were maintained in 10% FCS, 1% P/S, and 1% l‐glut Dulbecco's modified Eagle medium (DMEM). Lentiviral

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