MEF (C57BL/6) [MEF-BL/6-1] from ATCC

Product Specs
ItemMEF (C57BL/6) [MEF-BL/6-1]
CompanyATCC
Price $138.00Supplier Page View Company Product Page
Catalog NumberSCRC-1008
ApplicationsThe cells can be used as a feeder layer to support the growth of embryonic stem (ES) cells and for the maintenance of ES cells in the undifferentiated state. The growth of these cells must be arrested before they can be used as a feeder layer. ATCC has successfully treated the cells with mitomycin C for use as a feeder layer. If the MEFs are being used as a feeder layer for ES cells, it is not recommended to use them past passage no. 6 (P6)
TypeCell Biology
SpeciesMus musculus
TissueEmbryo
Cell Type (standardized)Fibroblasts
Cell Typefibroblast
Age14 days gestation

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Mouse TAPBPR shows functional similarity to human TAPBPR in shaping the MHC-I immunopeptidomeFront ImmunolApril 15, 2026Juliana Bernardi Aggio, Marcel Wacker, William Guo, Arwen F. Altenburg, Ida Hafstrand, Reem Satti, Alison McDowall, Aure Aflalo, Andreas Neerincx, Jack Welland, et al.USA), mouse melanoma B16-F10, Lewis lung carcinoma LL/2 and mouse stromal fibroblast MEF-BL/6-1 (ATCC SCRC-1008) cells were maintained in Dulbecco’s Modified Eagle’s medium (DMEM)(CAT: 41966052, Gibco™, Thermo Fisher

(2) Cereblon (CRBN) inhibits prostate cancer metastasis by negatively regulating 6-phosphogluconate dehydrogenase (6PGD)OncogeneMarch 9, 2026Koushik Guchhait, Hyeon-Seung Yoon, Hyun-Su An, Seungheon Shin, Hye Seung Nam, Francisco D. Yanqui-Rivera, Samara M. Oña, Miguel Á. Mendez, Jong Yeon Hwang, Daeho Park, et al.cell culture RWPE-1 (CRL-11609), DU145 (HTB-81), PC3 (CRL-1435), H1299 (CRL-5803), HEK293T (CRL-3216), MEF (SCRC-1008), and U-87 MG (HTB-14) cells were purchased from ATCC (Manassas, VA, USA). The LNCaP cell line was purchased

(3) BPGM shapes NFAT5-driven cellular responsesCellular and Molecular Life SciencesFebruary 25, 2026Kameliya Roegner, Vera A. Kulow, Ralf Mrowka, Kristina Engel, Bayram Edemir, Mumtaz Kasim, Cem Erdogan, Laetitia Malotka, Michael Fähling, Robert LabesCell culture and cell transfection For in vitro experiments mouse embryonic fibroblasts (MEF; ATCC-No.: #SCRC-1008, RRID: CVCL_9115) were used. This cell line shows robust BPGM expression and provides acceptable comparability

(4) Adolescent high fructose consumption induces cardiac dysfunction in adulthood via elevated histone acetylationTranslational PediatricsJanuary 31, 2026An-Na Shen, Hai-Qun Shi, Ze-Hua Zhang, Yao Yao, Rui Zhao, Yan Shi, Jian-Yuan Zhao, Ke Cai, Feng Wanganalysis. Cell culture H9c2 (Rat myocardial cells), MEF (Mouse embryonic fibroblast; ATCC Number: SCRC-1008) and NIH/3T3 (MEF cells; ATCC Number: CRL-1658) cells were used in this study. All cells were cultured

(5) Inhibition of Calcium‐Dependent Lipid Droplets Relocation of ACSL4‐PKCβ‐ALOX15 Complex Alleviates Ferroptosis and Acute PancreatitisAdvanced ScienceJanuary 27, 2026Guoyuan Hou, Jing Luan, Xiaoyong Xu, Jianhua Qin, Shuang Ma, Jiyuan He, Na Sun, Wei Zhang, Minghui Gaoglioblastoma U373 (HTB‐17), human breast adenocarcinoma MDA‐MB‐231 (HTB‐26), murine embryonic fibroblasts MEF (SCRC‐1008), human cardiomyocyte AC16 (CRL‐3568) and murine connective tissue L‐929 (CCL‐1). Cell lines were maintained

(6) Reducing Bias in Mass Cytometry Data through Corrections for Spillover and Nonspecific BindingAnalytical ChemistryJanuary 13, 2026Kris Elbein, Nameera Wegner, Edgar A. Arriagafollowing previous parameters.13 MEF CELL CULTURING AND STAINING Mouse embryonic fibroblasts (MEF, ATCC SCRC-1008) were grown in DMEM (Thermo Fisher) supplemented with 15% FBS (Gibco), 1000 units of penicillin, and

(7) Dynamins maintain nuclear envelope homeostasis and genome stabilityNature CommunicationsJanuary 8, 2026Célia Aveleira, Thibaud Martial, Loïc Carrique, Rita Gaspar, Ana Caulino-Rocha, Izaak Myatt, Franz Wendler, Pauline Lascaux, Misha Le Claire, James Bancroft, et al.4-hydroxytamoxifen (4-OHT; Sigma-Aldrich)19,20. Wild-type (WT) mouse (C57BL/6J) fibroblasts were obtained from ATCC (SCRC-1008). Dynamin TKO mouse fibroblasts and WT mouse fibroblasts were cultured in the growth medium: high glucose

(8) An In Vitro Assessment of a Biopolymer-Based Medical Foam for Enhanced Antifibrinolytic and Infection Prophylaxis for Acute Wound ManagementACS Applied Bio MaterialsDecember 15, 2025Amelia K. Stoner, Lynn M. Pezzanite, Steven W. Dow, Nicholas A. Alfonso, Kirk C. McGilvrayCytotoxicity Dose–response cytotoxicity profiles of the MF were generated for mouse embryonic fibroblasts (MEF, ATCC SCRC-1008) and human dermal fibroblasts (HDFa, Thermo Fisher) in accordance with ISO 10993-5 using Trypan Blue

(9) Anti-CRISPR-mediated continuous directed evolution of CRISPR-Cas9 in human cellsbioRxivDecember 11, 2025Andrew L. Sabol, Amanuella A. Mengiste, Vedagopuram Sreekanth, Prashant Singh, Samuel J. Hendel, Minh Thuan Nguyen Tran, Anton M. Barybin, Santosh Chaudhary, Ra’Mal M. Harris, Kristi Liivak, et al.select for and maintain transgenes. For the CASFISH assay, mouse embryonic fibroblasts (MEFs; ATCC SCRC-1008) were cultured in (DMEM; Gibco, Cat. No. 10564029) supplemented with 10% (v/v) fetal bovine serum (FBS;

(10) Cluster nanoarchitecture and structural diversity of PIEZO1 at rest and during activation in intact cellsScience advancesOctober 24, 2025Verkest C, Roettger L, Zeitzschel N, Hall J, Sánchez-Carranza O, Huang AT, Lewin GR, Lechner SG.penicillin-streptomycin (all from Thermo Fisher Scientific). U87 (U87-MG, ATCC HTB-14) and MEF (ATCC SCRC-1008) were grown in DMEM supplemented with 10% FBS and 1% penicillin-streptomycin. For some live TIRF imaging

(11) LRP1 Shedding in Ricin-Induced Lung Injury: A Cell-Specific Response to Toxin ExposureInt J Mol SciJune 6, 2025Anita Sapoznikov, Yentl Evgy, Moshe Aftalion, Reut FalachBethesda, MD, USA, 2017). 4.10. Mouse Embryonic Fibroblasts MEF cell line was purchased from ATCC (SCRC-1008, Manassas, VA, USA). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Biological Industries,

(12) Cytosolic cytochrome c represses ferroptosisCell metabolismJune 3, 2025Song X, Zhou Z, Liu J, Li J, Yu C, Zeh HJ, Klionsky DJ, Stockwell BR, Wang J, Kang R, et al.,Thermo Fisher Scientific,R70007 KPC,David Tuveson (Cold Spring Harbor Laboratory Cancer Cente),N/A MEF,ATCC,SCRC-1008 MEF cycs−/−,ATCC,CRL-2613 Pfa1,Marcus Conrad (Helmholtz Munich),N/A CFPAC1,ATCC,CRL-1918 Calu-1,ATCC,HTB-54 MC38,Sigma,SCC172 CX-1,National

(13) Salmonella exploits LRRK2-dependent plasma membrane dynamics to invade host cellsNature communicationsMarch 8, 2025Zhu H, Sydor AM, Yan BR, Li R, Boniecki MT, Lyons C, Cygler M, Muise AM, Maxson ME, Grinstein S, et al.tools and described in the ‘CRISPR Knockout’ section below. SH-SY5Y cells (ATCC CRL-2266), MEF (ATCC, SCRC-1008) cells and MCF-7 (ATCC, HTB-22) cells were obtained from ATCC. All cells used were authenticated and

(14) FTY720P-treated macrophages in PEG-4MAL hydrogels promote oral wound healingCytotherapyMarch 1, 2025Andre J. Burnham, Afra I. Toma, Daniel Shah, Tim Cha, Sundus Kaimari, Monica Behara, Keerthi Priya Chinniampalayam Sekar, Archana Kamalakar, Nick Willett, Edward Botchwey, et al.methods Cell culture Mouse RAW 264.7 cells (ATCC, TIB-71) and mouse embryonic fibroblast (MEF) (ATCC, SCRC-1008) were cultured with DMEM medium (Corning, 10013) supplemented with 10% fetal bovine serum (R&D Systems,

(15) Ezrin defines TSC complex activation at endosomal compartments through EGFR-AKT signalingElifeFebruary 12, 2025Giuliana Giamundo, Daniela Intartaglia, Eugenio Del Del Prete, Elena Polishchuk, Fabrizio Andreone, Marzia Ognibene, Sara Buonocore, Bruno Hay Hay Mele, Francesco Giuseppe Salierno, Jlenia Monfregola, et al.line (human),ARPE-19,ATCC,CRL-2302, Cell line (human),HeLa,ATCC,CCL-2, Cell line (mouse),MEF,ATCC,SCRC-1008, Cell line (human),HeLa EZR−/−,This paper,,See Materials and methods: Generation of an EZR−/− HeLa cell

(16) Beyond hemoglobin: Critical role of 2,3‐bisphosphoglycerate mutase in kidney function and injuryActa Physiol (Oxf)January 1, 2025Vera A. Kulow, Kameliya Roegner, Robert Labes, Mumtaz Kasim, Susanne Mathia, Claudia S. Czopek, Nikolaus Berndt, Philipp N. Becker, Gohar Ter‐Avetisyan, Friedrich C. Luft, et al.expression and, thus, are suitable for in vitro analysis of BPGM expression. MEF cells (ATCC‐No.: #SCRC‐1008, RRID: CVCL_9115) were cultured under sterile conditions at 37°C, 95% air, and 5% of carbon dioxide,

(17) Generation of induced pluripotent stem cells from the Asian batsInternational Journal of Veterinary Science and MedicineDecember 31, 2024Younsu Lee, Okjae Koo, Islam M. Saadeldintransferred with stretched glass Pasteur pipettes onto mouse embryonic fibroblast (MEF) feeder cells (Cat# SCRC-1008, ATCC, Manassas, VA, USA) and cultured using Stempan E14 GMEM (Product # P08–50600, PAN-Biotech GmbH,

(18) Migratory autolysosome disposal mitigates lysosome damageJournal of Cell BiologyDecember 2, 2024Takami Sho, Ying Li, Haifeng Jiao, Li YuVigofect (T001) was purchased from Vigorous. Cell culture and drug treatment Both MEF cell line (SCRC-1008; ATCC) and 293FT (RRID:CVCL_6911) cell line were cultured in Dulbecco’s modified Eagle medium (C11995500BT;

(19) Proteome profiling of polyomavirus nuclear replication centers using iPONDJournal of VirologyNovember 19, 2024Kimberly D. Erickson, Erika S. Langsfeld, Alexandra Holland, Christopher C. Ebmeier, Robert L. GarceaMETHODS Mammalian cell lines and culture C57 mouse embryonic fibroblasts (MEFs) were obtained from ATCC (SCRC-1008, Manassas, VA). MEFs were incubated at 37°C with 5% CO2 in a D10F complete medium consisting of Dulbecco’s

(20) Defining the activity of pro-reparative extracellular vesicles in wound healing based on miRNA payloads and cell type-specific lineage mappingMolecular therapy : the journal of the American Society of Gene TherapySeptember 4, 2024Park DJ, Choi W, Sayeed S, Dorschner RA, Rainaldi J, Ho K, Kezios J, Nolan JP, Mali P, Costantini T, et al.Uptake of FLAG-tagged EVs into primary MEFs was assessed by treatment of 1e6 cultured primary MEFs (Cat# SCRC-1008, ATCC) with 2 × 109 EV in a volume of 100 μL, incubated for 48 h, fixed, and stained with an anti-FLAG

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MEF (C57BL/6) [MEF-BL/6-1] from ATCC

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MEF (C57BL/6) [MEF-BL/6-1] from ATCC

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