Supplier Page from Creative Bioarray for COLO 320DMF
recommended medium: RPMI-1640 + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:6: trypsin/EDTA is not necessary as cells are partly in suspension and partly only loosely adherent; seed at 1-3 x 10^4 cells/cm2; 37 °C, 5% CO2.