Description
Mouse Embryonic Stem Cells (MSCs) are isolated from day 3.5 (E3.5) blastocyts of pathogen-free laboratory mice. The established ES cell lines are propagated by co-culture with mouse embryonic fibroblasts (MEF) as described in standard mouse ESC culture protocol. Mouse Embryonic Stem Cell-Derived Endothelial Cells (ES-ECs) from Creative Bioarray are isolated from MESCs. ES-EPCs are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Creative Bioarray's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.
ES-ECs can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications