Description
• Murine Embryonic Fibroblasts allow stem cell culture without LIF
• Presented as 5 x 10^6 cells in 1 mL
• Must be mitotically inactivated prior to addition of ES cells
Embryonic stem (ES) cells have been derived from the inner cellmasses (ICM) of blastocysts in many species. They are capable of unlimited, undifferentiated proliferation on feeder cell layers and remain karyotypically normal and phenotypically stable. In addition, ES cells have the ability to differentiate into a wide variety of cell types in vitro and in vivo. In mES cell culture, the feeder layer can be replaced by the addition of LIF in the growth medium. However, LIF does not have the same effect on hES cell culture as mES. Therefore, both the derivation and maintenance of hES cells require the use of feeder cells