Immortalized Human Cerebral Microvascular Endothelial Cell Line (hCMEC/D3) from CEDARLANE

Product Specs
ItemImmortalized Human Cerebral Microvascular Endothelial Cell Line (hCMEC/D3)
CompanyCEDARLANE
Price Supplier Page View Company Product Page
Catalog NumberCLU512
Quantity1 vial
Cell LinehCMEC/D3
Cell Type (standardized)Microvascular Endothelial Cells
Tissue (standardized)Brain
SpeciesHuman
TissueBrain
Cell TypeEndothelial Cells

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CEDARLANE

4410 Paletta Court

Burlington, Ontario L7L 5R2

Canada

Phone: +1 (289) 288-0001
Toll Free (North America): +1 (800) 268-5058
Fax: +1 (289) 288-0020

Email: general@cedarlanelabs.com

Company Profile

Website: http://www.cedarlanelabs.com/

(1) Temperature Dependence of Membrane Permeability Parameters for Five Cell Types Using Nonideal Thermodynamic Assumptions to Mathematically Model Cryopreservation ProtocolsThe Journal of Physical Chemistry BFebruary 8, 2024Faranak Yadegari, Laura A. Gabler Pizarro, Leah A. Marquez-Curtis, Janet A. W. Elliottparticle count and size analyzer. Human cerebral microvascular endothelial cells (hCMEC/D3 cell line, CLU512, Cedarlane, CELLutions Biosystems Inc., Burlington, ON, Canada) were received frozen and were immediately

(2) 3D blood-brain barrier-organoids as a model for Lyme neuroborreliosis highlighting genospecies dependent organotropismiScienceJanuary 20, 2023Yvonne Adams, Anne Skovsbo Clausen, Peter Østrup Jensen, Malin Lager, Peter Wilhelmsson, Anna J. Henningson, Per-Eric Lindgren, Daniel Faurholt-Jepsen, Helene Mens, Peter Kraiczy, et al.described.39,57,58,59,60 Human cell cultures The human brain microvascular endothelial cell line (hCMEC)/D3 (CLU512, Cedarlane Labs, CA.) was maintained on collagen-coated (50 μg/mL, rat tail type I, BD Biosciences)

(3) Recombinant human plasma gelsolin reverses increased permeability of the blood-brain barrier induced by the spike protein of the SARS-CoV-2 virusJournal of neuroinflammationNovember 24, 2022Suprewicz Ł, Tran KA, Piktel E, Fiedoruk K, Janmey PA, Galie PA, Bucki R.(North Brunswick, USA). Cell culture Immortalized human cerebral microvascular endothelial cells (#CLU512-A, Cedarlane Laboratories) were used between passage 25 and 35. Cells were cultured in EBM-2 medium

(4) Functional Expression of Multidrug-Resistance (MDR) Transporters in Developing Human Fetal Brain Endothelial CellsCellsJuly 21, 2022Lye P, Bloise E, Imperio GE, Chitayat D, Matthews SG.experiments. Adult human cerebral microvascular endothelial cell line (hCMEC/D3) cells (30 passages; CLU512, Cedarlane Labs, Burlington, ON, Canada) were cultured as previously described [25]. Briefly, hCMEC/D3

(5) Role of pericytes in blood–brain barrier preservation during ischemia through tunneling nanotubesCell Death & DiseaseJuly 5, 2022Francesco Pisani, Valentina Castagnola, Laura Simone, Fabrizio Loiacono, Maria Svelto, Fabio Benfenatilines Human cerebral microvascular endothelial cells (hCMEC/D3) were purchased from Cedarlane (cat. #CLU512) and cultured in endothelial basal medium (EBM-2; Lonza, cat. #190860) supplemented with 10% (v/v) fetal

(6) Hypoxia alters the expression of ACE2 and TMPRSS2 SARS-CoV-2 cell entry mediators in hCMEC/D3 brain endothelial cellsMicrovascular ResearchNovember 1, 2021Guinever E. Imperio, Phetcharawan Lye, Hafsah Mughis, Hirotaka Hamada, Enrrico Bloise, Stephen J. Lye, Stephen G. MatthewsCell culture and reagents The human cerebral microvascular endothelial cell line (hCMEC/D3, Cedarlane Labs #CLU512, Burlington, Canada) was cultured at 37 °C, in 5% CO2 and 20% O2 with EndoGRO™-MV Complete Culture Media

(7) Function of Multidrug Resistance Transporters is Disrupted by Infection Mimics in Human Brain Endothelial CellsTissue barriersApril 3, 2021Eustaquio Do Imperio G, Lye P, Bloise E, Matthews SG.Cell culture and reagents The human cerebral microvascular endothelial cell line (hCMEC/D3, Cedarlane Labs #CLU512, Burlington, ON, Canada) was selected as a model to investigate drug transporters function since it

(8) Plasmodium falciparum erythrocyte membrane protein 1 variants induce cell swelling and disrupt the blood–brain barrier in cerebral malariaJournal of Experimental MedicineMarch 1, 2021Yvonne Adams et al.et al., 2010). Human cell cultures The human brain microvascular endothelial cell line hCMEC/D3 (CLU512; Cedarlane Labs) was maintained in collagen-coated (50 µg/ml; rat tail type I; BD Biosciences) flasks.

(9) Cryopreservation of human cerebral microvascular endothelial cells and astrocytes in suspension and monolayersPloS oneJanuary 1, 2021Marquez-Curtis LA, Bokenfohr R, McGann LE, Elliott JAW.and human astrocytes The human cerebral microvascular endothelial cell/D3 clone (hCMEC/D3 cell line, CLU512, Cedarlane, CELLutions Biosystems Inc., Burlington, ON, Canada) was purchased as cryopreserved cells

(10) Blood–brain-barrier organoids for investigating the permeability of CNS therapeuticsNature ProtocolsJuly 21, 2019Sonja Bergmann, Sean E. Lawler, Yuan Qu, Colin M. Fadzen, Justin M. Wolfe, Michael S. Regan, Bradley L. Pentelute, Nathalie Y. R. Agar, Choi-Fong Choby commercial STR. Human cerebral microvascular endothelial cell (hCMEC/D3) (Cedarlane, cat. no. CLU512) or primary human brain microvascular endothelial cells (HBMEC) (ScienCell, cat. no. 1000)! CAUTION

(11) α-Synuclein pre-formed fibrils impair tight junction protein expression without affecting cerebral endothelial cell functionExperimental neurologyNovember 1, 2016Kuan WL, Bennett N, He X, Skepper JN, Martynyuk N, Wijeyekoon R, Moghe PV, Williams-Gray CH, Barker RA.running Deben software (AMT, Woburn, USA). Cell cultures hCMEC/D3 human brain endothelial cells (Cedarlane, CLU512) were cultured in T25 flasks coated with 150 μg/mL rat Collagen I lower viscosity (R&D systems, 3443-10001)

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Immortalized Human Cerebral Microvascular Endothelial Cell Line (hCMEC/D3) from CEDARLANE

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Immortalized Human Cerebral Microvascular Endothelial Cell Line (hCMEC/D3) from CEDARLANE

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