NEB 5-alpha Competent E. coli (High Efficiency) from New England Biolabs

Product Specs
ItemNEB 5-alpha Competent E. coli (High Efficiency)
CompanyNew England Biolabs
Price $503.00 Inquire
Catalog NumberC2987P
Quantity1 96-well plate
ApplicationsDNA Manipulation
Cell Type (standardized)Competent Cells
SpeciesEscherichia
Temperature-80 C
Cell TypeCompetent Cells

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) FUT1- and GAL3ST2-mediated cellular glycan remodeling broadly restricts sialic acid–dependent viral infectionsProceedings of the National Academy of SciencesMarch 10, 2026Xinyu Zhu, Satoko Nakano, Vishwas N. Rao, Hector A. Miranda, M. Ariel Spurrier, Samantha Skavicus, Zhaochen Luo, Nicholas S. HeatonE. coli (Agilent, 200152) to increase stability. Other plasmids were both propagated in DH5α (NEB, C2987P). Virus infections Influenza A virus H1N1, H3N2 and H5N1, including PR8, A/Hawaii/70/2019, A/Wyoming/03/2003,

(2) Rapid parallel reconstruction and specificity screening of hundreds of T cell receptorsNature ProtocolsMarch 1, 2025Alexander B. Afeyan, Catherine J. Wu, Giacomo OliveiraCenter IRB, 14–36223. NEB 5-alpha Competent E. coli and SOC medium (NEB, Plate (Step 24): cat. no. C2987P, Tubes (Step 19): C2987H) Lenti-X 293T cells (Takara Bio, cat. no. 632180) !CAUTION: Lentiviral work

(3) DdmDE eliminates plasmid invasion by DNA-guided DNA targetingCellSeptember 19, 2024Xiao-Yuan Yang, Zhangfei Shen, Chen Wang, Kotaro Nakanishi, Tian-Min FuOrganisms/strains,Experimental models: Organisms/strains E. coli DH5α Chemically Competent Cell,NEB,C2987P E. coli BL21 (DE3) Chemically Competent Cell,NEB,C2527H ,, Oligonucleotides,Oligonucleotides,Oligonucleotides "Oligonucleotides

(4) Developing a platform for secretion of biomolecules in Mycoplasma feriruminatorisMicrobial Cell FactoriesApril 30, 2024Javier Gonzalez-de-Miguel, Ariadna Montero-Blay, Ludovica Ciampi, Irene Rodriguez-Arce, Luis SerranoPurification kit (Lucigen, MC85200). Clones were isolated into NEBVR 5-alpha E. coli (New England Biolabs, C2987P) using heat shock protocol. Plasmids were purified using NZY Miniprep kit, (MB01001) following the manufacturer’s

(5) Assembly-mediated activation of the SIR2-HerA supramolecular complex for anti-phage defenseMolecular CellDecember 13, 2023Zhangfei Shen, Qingpeng Lin, Xiao-Yuan Yang, Elizabeth Fosuah, Tian-Min FuStrains,Bacterial and Virus Strains,Bacterial and Virus Strains E. coli DH5α Chemically Competent Cell,NEB,C2987P E. coli BL21 (DE3) Chemically Competent Cell,NEB,C2527H Escherichia phage T5,DSMZ,DSM 16353 Deposited

(6) HORMAD1 overexpression predicts response to anthracycline–cyclophosphamide and survival in triple‐negative breast cancersMolecular OncologyOctober 1, 2023Rania El‐Botty, Sophie Vacher, Juliette Mainguené, Adrien Briaux, Sabrina Ibadioune, Ahmed Dahmani, Elodie Montaudon, Fariba Nemati, Léa Huguet, Laura Sourd, et al.pCMV6‐Myc‐Flag were mixed with 25 μL of competent bacteria (Bacteria NEB® 5‐alpha Competent E. coli, #C2987P New England Biolabs) and incubated for 45 min on ice. A heat shock was then performed for 45 s at 42

(7) Deep mutagenesis scanning using whole trimeric SARS-CoV-2 spike highlights the importance of NTD-RBD interactions in determining spike phenotypePLoS pathogensAugust 1, 2023Kugathasan R, Sukhova K, Moshe M, Kellam P, Barclay W.Assembly (NEB #E2621). The assembled products were used to transform NEB DH5α ultracompetent cells (NEB # C2987P), following a 1-hour period of outgrowth at 37°C, the outgrowth media and transformed cells were poured

(8) ZBTB7A promotes virus-host homeostasis during human coronavirus 229E infectionCell reportsOctober 25, 2022Zhu X, Trimarco JD, Williams CA, Barrera A, Reddy TE, Heaton NS.expression plasmid through HiFi DNA assembly (NEB, M5520AA). Plasmid DNA was propagated in DH5α (NEB, C2987P) and all plasmid clones were verified by Sanger sequencing. Lentiviral production and transduction The

(9) Protective neutralizing antibodies from human survivors of Crimean-Congo hemorrhagic feverCellJune 24, 2021Fels JM, Maurer DP, Herbert AS, Wirchnianski AS, Vergnolle O, Cross RW, Abelson DM, Moyer CL, Mishra AK, Aguilan JT, et al.resulting plasmid pools were transformed into chemically competent E. coli in 96-well plates (NEB, Cat#C2987P). After overnight growth of E. coli, the plasmid pools were isolated by a 96-well miniprep kit (Macherey-Nagel,

(10) Interactions between lineage-associated transcription factors govern haematopoietic progenitor statesThe EMBO journalDecember 15, 2020Kucinski I, Wilson NK, Hannah R, Kinston SJ, Cauchy P, Lenaerts A, Grosschedl R, Göttgens B.a 20 μl reaction. Ligation was incubated at 16°C overnight and transformed into DH5α bacteria (NEB C2987P). Clones were isolated and verified by sequencing. CRISPR/Cas9 perturbation To perturb TFs in Hoxb8‐FL+Cas9

(11) DNA-BOT: a low-cost, automated DNA assembly platform for synthetic biologySynthetic BiologyJuly 9, 2020Marko Storch, Matthew C Haines, Geoff S Baldwinmin in a peqSTAR 96X Universal Gradient thermocycler. NEB® 5-alpha Competent E. coli, 96 well plates (C2987P) were used for transformation reactions. Heat shocks were conducted according to the manufacturer’s

(12) Inferring Active Metabolic Pathways from Proteomics and Essentiality DataCell ReportsJune 2, 2020Ariadna Montero-Blay, Carlos Piñero-Lambea, Samuel Miravet-Verde, Maria Lluch-Senar, Luis Serranothe Gibson assembly method (Gibson et al., 2009). DNA was isolated from NEB® 5-alpha High Efficiency (C2987P). Clones were isolated using LB agar plus ampicillin (100 μg/ml) plates and confirmed by sequencing

(13) SynMyco transposon: engineering transposon vectors for efficient transformation of minimal genomesDNA ResearchAugust 1, 2019Ariadna Montero-Blay, Samuel Miravet-Verde, Maria Lluch-Senar, Carlos Piñero-Lambea, Luis SerranoSupplementary Table S1. For plasmid generation, DNA was isolated from NEB® 5-alpha High Efficiency (C2987P). The clones were isolated using LB agar + ampicillin (100 µg/ml) plates and confirmed by sequencing

(14) Standardizing Automated DNA Assembly: Best Practices, Metrics, and Protocols Using RobotsSLAS TechnologyJune 1, 2019David I. Walsh et al.Transformations were performed by adding 2 μL of each reaction to 20 μL of NEB 5-alpha competent cells (C2987P), following vendor’s instructions. 10% of each resulting culture (20 μL) was mixed with 30 μL of LB

(15) Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9Molecular cellMay 18, 2017Yang CS, Jividen K, Spencer A, Dworak N, Ni L, Oostdyk LT, Chatterjee M, Kuśmider B, Reon B, Parlak M, et al.and Virus Strains,Bacterial and Virus Strains DH5α (general plasmid preparation),New England BioLabs,C2987P BL21(DE3)pLysS,EMD Millipore (Novagen),69451 "Chemicals, Peptides, and Recombinant Proteins","Chemicals,

(16) A mutagenesis and screening strategy to generate optimally thermostabilized membrane proteins for structural studiesNature ProtocolsAugust 1, 2016Francesca Magnani, Maria J. Serrano-Vega, Yoko Shibata, Saba Abdul-Hussein, Guillaume Lebon, Jennifer Miller-Gallacher, Ankita Singhal, Annette Strege, Jennifer A. Thomas, Christopher G. TateD310) • DpnI (NEB, R0176S) • DH5-alpha Competent E. coli (High efficiency) 1x96 well plate (NEB, C2987P) • Dulbecco’s Modified Eagle Medium (DMEM), GLUTMAX™ supplement, pyruvate (life technologies 31966-0210) • Foetal

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