Description
Bone is a dynamic tissue, being continuously remodeled by the coordinated actions of osteoclasts and osteoblast. Osteoblasts, the bone-forming cells, are derived originally from pluripotent mesenchymal stem cells. They synthesize and secrete organic extracellular matrix, osteoid, which is composed primarily of type I collagen. Osteoid is calcified by osteoblasts and during this process the cells become encased in lacunae within the calcified material and become osteocytes. Osteoblasts express protease-activated receptor-1 and vascular endothelial cell growth factor [1]. Studies show that leukemia inhibitory factor can bind to the osteoblast cell surface and induce bone formation both in vitro and in vivo [2]. The balance between osteoblast recruitment, proliferation, differentiation and apoptosis in sutures between cranial bones is essential for calvarial bone formation [3]. HO-f from ScienCell Research Laboratories are isolated from human femur. HO-f are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HO-f are characterized by the cytochemical detection of AP and mineral deposition. HO-f are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HO-f are guaranteed for 15 population doublings under the conditions provided by ScienCell Research Laboratories. Recommended Medium: It is recommended to use Osteoblast Medium (OsM, Cat. #4601) for the culturing of HO-f in vitro