Description
Renal proximal tubular epithelial cells (RPTEpiC) play a crucial role in renal function. They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate, while allowing other substances of no nutritional value to be excreted in the urine. They are also a major site of injury in a variety of congenital, metabolic, and inflammatory diseases. RPTEpiC can produce inflammatory mediators such as cytokines or chemokines and actively participate in acute inflammatory processes by affecting and directing leukocyte chemotaxis via the production of IL-8 [1, 2]. RPTEpiC express IL-2R alpha and MHC class II antigens during inflammation after renal transplantation or in crescentic glomerulonephritis, indicating the capacity to participate in the pathogenesis of immune renal injury [3]. To be able to study the relationship between proximal tubular cells and a variety of renal diseases, the RPTEpiC culture provides a useful in vitro model. RRPTEpiC from ScienCell Research Laboratories are isolated from neonate day 2 CD® IGS rat kidney tissue. RRPTEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. RRPTEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, -19 and vimentin. RRPTEpiC are negative for mycoplasma, bacteria, yeast and fungi. RRPTEpiC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, RRPTEpiC are not recommended for long-term cultures due to limited expansion capacity and senescence after subculturing. Recommended Medium It is recommended to use Epithelial Cell Medium-animal (EpiCM-a, Cat. #4131) for expanding RRPTEpiC in vitro