Description
The development of the cerebellum involves a set of coordinated cell movements and two separate proliferation zones: the ventricular zone and the external granule cell layer (EGL), a rhombic-lip-derived progenitor pool [1]. The EGL appears segregated during early cerebellum formation and produces only granule cells. Cerebellar granule cells (CGC) are the most abundant neurons in the brain, about 1 x 10^11 in humans [2]. Their axons run as parallel fibres along the coronal axis, and the one-dimensional spread of excitation that results from this arrangement is a key assumption in theories of cerebellar function. CGC receive inhibitory synaptic input from Golgi cells, which are mediated by gamma-aminobutyric acid (GABA). During both in vivo and in vitro development, CGC depend on the activity of the NMDA glutamate receptor subtype for survival and full differentiation [3]. Cultured CGC are widely used as a model system for studying neuronal apoptosis. HCGC from ScienCell Research Laboratories are isolated from human cerebellum. HCGC are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HCGC are characterized by immunofluorescence with antibodies specific to neurofilament, MAP2, and β-tubulin III. HCGC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HCGC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HCGC are not recommended for expanding or long-term cultures since the cells do not proliferate in culture. Recommended Medium: It is recommended to use Neuronal Medium (NM, Cat. #1521) for culturing HCGC in vitro