Description
The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain [1]. Despite great variability in size and shape, all neurons share common morphological features, the key elements of a highly complex communication network. Neurons are dynamically polarized cells that serve as the major signaling unit of the nervous system. The brain contains about 1 x 10^11 neurons, each are able to contact at least 10,000 other neurons [2]. RN-dsc from ScienCell Research Laboratories are isolated from the embryonic day 14 CD® IGS rat spinal cord. RN-dsc were cryopreserved as primary cultures and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. RN-dsc are characterized by immunofluorescence with antibodies specific to neurofilament, MAP2, and β-tubulin III. RN-dsc are negative for mycoplasma, bacteria, yeast, and fungi. RN-dsc are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, RN-dsc are not recommended for expanding or long-term cultures since the cells do not proliferate in culture. Recommended Medium It is recommended to use neuronal medium (NM, Cat. #1521) for culturing RN-dsc in vitro