Fig 1: Influence of the position of the splGFP11 tag on the SplitGFP signal. The highest expressed NOD2 construct, ND1 was tagged N‐ or C‐ terminally with different spacer elements with the splGFP11 tag. The resulting SplitGFP response was measured in the BioLector. The BioLector sensitivity was slightly increased compared to the data shown in Figure 3 to accommodate the lower GFP signal of the C‐terminal tagged constructs.
Fig 2: Analysis of the NOD2 expression. Western Blots for the intracellular soluble and insoluble protein fraction of the NOD2 constructs obtained with BEVS. The N‐terminal Strep tagged NOD2 constructs were produced under regulation of the p10 promoter in Hi5 cells under controlled conditions. The expression was compared with the max. NFI determined in the BioLector experiment.
Fig 3: Relative NFI levels of the NOD2 constructs. A schematic representation of the NOD2 protein with predicted domain boundaries is shown. Forty‐two different NOD2 constructs with different boundaries were designed and fused to splGFP11. The SplitGFP screen in the BioLector showed the difference in expression level for each construct. The diagram below depicts the average max. NFI of at least three or more BioLector measurements.
Fig 4: Comparison of the GFP response of nine different C‐ and N‐terminal splGFP11 tagged NOD2 constructs. The BioLector sensitivity was slightly increased compared to the data shown in Figure 3 to accommodate the lower GFP signal of the C‐terminal tagged constructs.
Supplier Page from DNASU for NOD2 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)