Fig 1: BspF localizes to the endosomal recycling compartment Representative confocal fluorescence micrograph of HeLa cells co-transfected for 24 h to produce mCherry-BspF and GFP-BspF and treated with Cytochalasin D (200 nM) for 30 min prior to fixation. Scale bars: 10 and 2 µm (insets).Representative confocal fluorescence micrographs of HeLa cells co-transfected for 24 h to produce mCherry-BspF and either GFP-MICAL-L1, GFP-STX16, GFP-STX6, or GFP-VAMP3 and treated with Cytochalasin D (200 nM) for 30 min prior to fixation. Scale bars: 10 and 2 µm (insets). Localization of GFP-MICAL-L1, GFP-STX16, GFP-STX6, or GFP-VAMP3 to mCherry-BspF-labeled tubules was quantified in at least 300 individual cells per experiment. Data are means ± SD from n = 3 independent experiments.Representative confocal fluorescence micrographs of HeLa cells co-transfected for 24 h to produce mCherry-BspF and either GFP-Rab11a or VAMP4-GFP and treated with Cytochalasin D (200 nM) for 30 min prior to fixation. Scale bars: 10 and 2 µm (insets). Localization of GFP-Rab11a or VAMP4-GFP to mCherry-BspF-labeled tubules was quantified in at least 300 individual cells per experiment. Data are means ± SD from n = 3 independent experiments.