Endo H V4871 from Promega | Biocompare.com

Product Specs
ItemEndo H
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV4871
Size10,000 units

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Breast cancer bone metastasis and bone metastatic cells retain NKG2DLs intracellularly: could this be a strategy to evade immune recognition?Frontiers in Cell and Developmental BiologyJanuary 27, 2026Marta Gomarasca, Paola Maroni, Chiara Verdelli, Laura Gerosa, Martina Faraldi, Alessandro Luzzati, Luca Cannavò, Giuseppe Banfi, Giovanni Lombardior 50 μg of total protein from breast cancer cell lysates were digested with either 1500 U of EndoH (V4871, Promega) for 4 h or 20 U of PNGase F (V4831, Promega) for 2 h, according to the manufacturer’s instructions.

(2) Exploration of Bromodomain Proteins as Drug Targets for Niemann-Pick Type C DiseaseInt J Mol SciJune 16, 2025Martina Parente, Amélie Barthelemy, Claudia Tonini, Sara Caputo, Alessandra Sacchi, Stefano Leone, Marco Segatto, Frank W. Pfrieger, Valentina Pallottinicentrifuged at 12,000 rpm for 10 min at 4 °C to yield total lysate. The endoglycosidase H (EndoH) assay (V4875, Promega Italia; Milano, Italy) was performed following the manufacturer’s instructions. The samples

(3) Spc2 modulates substrate- and cleavage site-selection in the yeast signal peptidase complexJournal of Cell BiologyDecember 2, 2024Yeonji Chung, Chewon Yim, Gilberto P. Pereira, Sungjoon Son, Lisbeth R. Kjølbye, Lauren E. Mazurkiewicz, Amy M. Weeks, Friedrich Förster, Gunnar von Heijne, Paulo C.T. Souza, et al.Quartett], and bromophenol blue) and incubated at 60°C for 15 min. Samples were incubated with Endo H (Cat# V4871; Promega or Cat# P0703L; NEB) at 37°C for 30–60 min. Western blot analysis Proteins were prepared

(4) DRG2 is required for surface localization of PD-L1 and the efficacy of anti-PD-1 therapyCell death discoveryMay 27, 2024Choi SH, Mani M, Kim J, Cho WJ, Martin TFJ, Kim JH, Chu HS, Jeong WJ, Won YW, Lee BJ, et al.as described above. Cell lysates were treated with 1.000 unit endoglycosidase H (endo H) (Promega, V4871) for 17 h at 37 °C in the presence the buffers recommended by the supplier. Samples were loaded onto

(5) DAYWAKE implicates novel roles for circulating lipid‐binding proteins as extracerebral regulators of daytime wake–sleep behaviorFEBS LettersFebruary 1, 2024Gabriel Villegas, Mathew T. Pereira, Cameron R. Love, Isaac Ederymedia). Endoglycosidase H treatment of DAYWAKE produced in S2 cells. We used Endo H (Promega, cat# V4871) and followed manufacturer’s protocol. Essentially, 20μg of total protein extract generated from spun

(6) Recombinant rotavirus expressing the glycosylated S1 protein of SARS-CoV-2Journal of General VirologyOctober 13, 2023Asha A. Philip, Sannoong Hu, Jin Dai, John T. Pattonproteins in the cell lysates was assessed using Promega Endoglycosidase H (Endo H) assay reagents (Promega V4871). Briefly, 27 µl samples of cell lysates were combined with 3 µl of 10X Denaturing Solution, heated

(7) Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 ReceptorbioRxivApril 26, 2023Peng Zhao, Jeremy L. Praissman, Oliver C. Grant, Yongfei Cai, Tianshu Xiao, Katelyn E. Rosenbalm, Kazuhiro Aoki, Benjamin P. Kellman, Robert Bridger, Dan H. Barouch, et al.,Sigma Aldrich,Cat#I1149 Trypsin,Promega,Cat#V5111 Lys-C,Promega,Cat#V1671 Arg-C,Promega,Cat#V1881 Glu-C,Promega,Cat#V1651 Asp-N,Promega,Cat#VA1160 Endoglycosidase H,Promega,Cat#V4871 PNGaseF,Promega,Cat#V4831 Chymotrypsin,Athens Research and Technology,Cat#16-19-030820 Alpha lytic

(8) Altered energy metabolism in Fatal Familial Insomnia cerebral organoids is associated with astrogliosis and neuronal dysfunctionPLOS GeneticsJanuary 19, 2023Simote T. Foliaki, Anna Smith, Benjamin Schwarz, Eric Bohrnsen, Catharine M. Bosio, Katie Williams, Christina D. Orrú, Hailey Lachenauer, Bradley R. Groveman, Cathryn L. HaighNew England BioLabs PNGaseF kit (P0704S), and the Endo H digest was performed using a kit from Promega (Cat. V4875). About 9 μl lysate (20% w/v in 1× PBS) was denatured by boiling for 10 minutes in 1× denaturing buffer

(9) 2-deoxyglucose transiently inhibits yeast AMPK signaling and triggers glucose transporter endocytosis, potentiating the drug toxicityPLOS GeneticsAugust 11, 2022Clotilde Laussel, Véronique Albanèse, Francisco Javier García-Rodríguez, Alberto Ballin, Quentin Defenouillère, Sébastien LéonSamples were incubated at 37°C for 3 h, and loaded on SDS-PAGE. Endoglycosidase H (EndoH) (Promega V4871, 500 U/μL) was used to deglycosylate Ina1-GFP-containing samples. Samples were prepared using 10 μL

(10) Alternative redox forms of ASNA-1 separate insulin signaling from tail-anchored protein targeting and cisplatin resistance in C. elegansScientific ReportsDecember 1, 2021Dorota Raj, Ola Billing, Agnieszka Podraza-Farhanieh, Bashar Kraish, Oskar Hemmingsson, Gautam Kao, Peter Narediconcentration determination. The lysate was split in two and one half treated with Endoglycosidase H (V4871, Promega) as described by the supplier. All samples were solubilized in Laemmli buffer, separated by

(11) Glycomics-informed glycoproteomic analysis of site-specific glycosylation for SARS-CoV-2 spike proteinSTAR ProtocolsDecember 30, 2020Katelyn E. Rosenbalm, Michael Tiemeyer, Lance Wells, Kazuhiro Aoki, Peng Zhaoreagent",Sigma-Aldrich,320331 Dithiothreitol,Sigma-Aldrich,43815 Iodoacetamide,Sigma-Aldrich,I1149 Trypsin,Promega,V5111 Lys-C,Promega,V1671 Arg-C,Promega,V1881 Glu-C,Promega,V1651 Asp-N,Promega,VA1160 Endoglycosidase H,Promega,V4871 PNGaseF,Promega,V4831 Chymotrypsin,Athens Research and Technology,16-19-030820 Alpha lytic protease,New England BioLabs

(12) Recombinant N-glycosylation isoforms of Legume lectins: Production and purification from Nicotiana benthamiana leaves following RuBisCO depletionPlant Physiology and BiochemistryDecember 1, 2020Kevin Bellande, Alexandre Lalo, Lætitia Ligat, David Roujol, Elisabeth Jamet, Hervé CanutPeptide N-Glycosidase F (PNGaseF) according to the instructions supplied by the manufacturer (EndoH, V4871; PNGaseF, V483A; Promega). The purified protein samples (0.5 – 2 µg) were first denaturated for 5 min

(13) Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 ReceptorCell Host & MicrobeOctober 1, 2020Peng Zhao, Jeremy L. Praissman, Oliver C. Grant, Yongfei Cai, Tianshu Xiao, Katelyn E. Rosenbalm, Kazuhiro Aoki, Benjamin P. Kellman, Robert Bridger, Dan H. Barouch, et al.,Sigma Aldrich,Cat#I1149 Trypsin,Promega,Cat#V5111 Lys-C,Promega,Cat#V1671 Arg-C,Promega,Cat#V1881 Glu-C,Promega,Cat#V1651 Asp-N,Promega,Cat#VA1160 Endoglycosidase H,Promega,Cat#V4871 PNGaseF,Promega,Cat#V4831 Chymotrypsin,Athens Research and Technology,Cat#16-19-030820 Alpha lytic

(14) PRMT5 Is Required for Bovine Leukemia Virus Infection In Vivo and Regulates BLV Gene Expression, Syncytium Formation, and Glycosylation In VitroVirusesJune 16, 2020Wlaa Assi, Tomoya Hirose, Satoshi Wada, Ryosuke Matsuura, Shin-nosuke Takeshima, Yoko Aidaor Endo H (Promega) overnight according to the manufacturer’s protocol (catalog# V483A and catalog #V4871), respectively. 2.9. Cell Viability Assay A Premix WST-1 Cell Proliferation Assay Kit (Takara, Shiga,

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Endo H

Endo H from Promega

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Promega

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Endo H from Promega

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