Trypsin/Lys-C Mix, Mass Spec Grade V5071 from Promega

Product Specs
ItemTrypsin/Lys-C Mix, Mass Spec Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV5071
Size20microg

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) BOLD-100 treatment induces systemic lipid crosstalk in metastatic colorectal carcinoma patients in a combination treatment with FOLFOXiScienceSeptember 18, 2026Yasmin Borutzki, Gerhard Hagn, Andrea Bileck, Thomas Mohr, Mark Bazett, Malcolm Snow, Michelle Jones, Jim Pankovich, Christopher Gerner, Bernhard K. Keppler, et al.C4706 2-chloroacetamide (2-CAM),Alfa Aesar,Cat# 11462278 "Trypsin/Lys-C Mix, Mass Spec Grade",Promega GmbH,Cat# V5071 Isopropanol,VWR Chemicals,Cat# 20839 Trifluoroacetic acid (TFA),Sigma Aldrich,Cat# 302031 Empore™

(2) Phosphoproteomic Insights into TRPV4-AMPK Signaling Axis and Barrier Regulation in the Choroid Plexus EpitheliumInt J Mol SciSeptember 18, 2026Gowthami Mahendran, Maryam Torabi, Bonnie L Blazer-Yostprotease-to-substrate ratio, mass Spectrometry-grade, Promega Corporation, Madison, WI, USA, Cat No: V5072.) Digestions were acidified with trifluoroacetic acid (TFA, 0.5% v/v) and desalted on Sep-Pak® Vac cartridges

(3) MORC1 coordinates TRIM28 interaction and nuclear condensate formation for transposon silencingiScienceSeptember 18, 2026Yosuke Isota, Peilin Li, Kiichiro Sakata, Hiyu Chiwata, Hidetaka Kosako, Soichiro Yamanaka, Shinsuke Ito, Hiroya Yamazaki, Mikiko C. SiomiKit,Illumina,Cat# FC-121-1030 MinElute PCR Purification Kit,Qiagen,Cat# 28004 Trypsin/Lys-C Mix,Promega,Cat# V5072 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, ChemiDoc

(4) Bacterial-derived cyclic lipopeptides suppress dauer arrest in C. elegansbioRxivSeptember 16, 2026Xiao Wang, Kelsie M. Nauta, Marco E. Mechan-Llontop, Darrick R. Gates, Nirmal Chaudhary, Amudha Ramchandran, Kersten K. Sykes, Corrie L. Sakowski, Vashnie H. Hartwell, Jade Tarango, et al.minutes, alkylated with Iodoacetamide (IAA) for 20 minutes and digested overnight with Trypsin/Lys-C (Cat# V5072, Promega) at a ratio of 50:1 (protein:enzyme (w/w)). Peptides were eluted off the S-Trap Column and

(5) An Unbiased Geometry-Resolved Membrane Platform for Proteome-Scale Discovery of Membrane Curvature SensorsAdv Sci (Weinh)September 8, 2026Takumi Komikawa, Rikuto Kawakami, Kunanon Chattrairat, Tatsuya Niwa, Taiga Ajiri, Takao Yasui, Masayoshi TanakamM ammonium bicarbonate buffer containing 0.8 µg of Trypsin/Lys‐C Mix (Promega, Madison, USA, Cat. #V5072) and incubated overnight at 37°C. After digestion, peptides were collected by centrifugation at 15 000

(6) Protocol for N-glycoproteomic enrichment of cancer cell-surface proteins in patient-derived xenograftsSTAR ProtocolsSeptember 1, 2026Konrad Celejewski, Meinusha Govindarajan, Matthew Waas, Thomas KislingerCarboxylate-Modified Magnetic Beads & SpeedBeads,Cytiva,45152105050250 "Trypsin/Lys-C Mix, Mass Spec Grade",Promega,V5071 "Invertase, from baker’s yeast (S. cerevisiae)",Sigma-Aldrich,I4504 Iodoacetamide,Sigma-Aldrich,I6125 Dithiothreitol,Bioshop,3483-12-3 HPLC-grade

(7) Protocol for isolation of interstitial fluid from tumor-draining lymph nodes for proteomic profiling in murine breast cancer modelsSTAR ProtocSeptember 1, 2026Greta Mattavelli, Jonathan J. Swietlik, Moutaz Helal, Arpa Aintablian, Felix Meissner, Angela RiedelW6500 Empore™ SPE Disks (SDB-RPS),Sigma Aldrich,Cat# 66886-U "Trypsin/Lys-C Mix, Mass Spec Grade",Promega,Cat# V5071 Isoflurane - Iso-Vet 1000 mg/g,Dechra,N/A Metamizole HEXAL® 500 mg/ml,Hexal,N/A Bepanthen® Eye and

(8) Method Matters: A Proteomics-Informed Framework for Selecting Extracellular Vesicle Isolation Methods for PlasmaJ Extracell BiolAugust 29, 2026Scheila Julia Werle, Marie Louise Nautrup Therkelsen, Chen Meng, Mads Grønborg, Lise Lotte Gluud, Dres Damgaardexchange to PBS. A buffer comprising 10% n‐Dodecyl β‐D‐Maltoside (DDM), Trypsin/Lys‐C (Promega, Cat# V5071), and 50 mM triethylammonium bicarbonate (TEAB) were added to the filter tube for the lysis of retained

(9) Members of the RhoA subfamily of GTPases suppress the stimulatory effect of the HPV E6 oncoprotein on the ubiquitin ligase E6APSci RepAugust 25, 2026Daniela Eichbichler, Jasmin Jansen, Nadja Eulich, Florian Stengel, Martin Scheffneriodoacetamide for 30 min at 23 °C in the dark. After dilution of SOD to 1%, Lys-C/trypsin mix (Promega V5071, Sequencing grade) was added at an E:S ratio of 1:50 and incubated for 18 h at 37 °C. The enzymatic

(10) Quantitative proteomic analysis of the anti-ferroptosis effect of a proprietary hydroxy-α-sanshool extract in human keratinocytes using a tandem mass tag labeling approachRSC AdvAugust 14, 2026Huifang Li, Xian Jiang, Ang Cai, Chang Liu, Hang Mawas then transferred to a new tube for digestion. Trypsin/Lys-C protease (Mass Spec Grade, Promega V5071) was added to the sample to a final volume of 125 µL, and the sample was incubated overnight at 37 °C

(11) N-Glycoproteomics sample preparation using commercially available cellulose magnetic particlesMolecular & Cellular ProteomicsAugust 11, 2026Ken Hanzawa, Miki Tanaka-Okamoto, Matej Nemcic, Yasuhide Miyamoto, Kazuki Nakajima(LC-MS) were purchased from Kanto Chemical. ReliaPrep Resin (#A175B) and Trypsin/Lys-C Mix, MS Grade (#V5072) were purchased from Promega. Sera-Mag SpeedBead carboxylate-modified [E3] and Sera-Mag SpeedBead carboxylate-modified

(12) TRPA1 deficiency alters brown adipose tissue proteome and thermogenic gene expression during cold exposure in miceBiochem Biophys RepAugust 7, 2026Cristhian D. Sua-Cespedes, Otoniel Gonçalves Lima, Letícia Menezes Vasconcelos, Giovanna Zanetti, Miriane Avelino da Silva, Maria Nathalia Moraes, Guilherme Gomes, William Tadeu Festuccia, Ana Maria L. Castrucci, José Thalles LacerdaTween 20,Sigma-Aldrich,P2287 Urea,Merck,1.08487.1000 Iodoacetamide,Sigma-Aldrich,I6125 NH4HCO3,Sigma-Aldrich,A6141 Trypsin/LysC Mix,Promega,V5071 Trypsin,Promega,V5280 Acetonitrile,Sigma-Aldrich,900667 Trifluoroacetic acid,ThermoScientific,28901 C18

(13) Proteome Remodeling of a Carbapenem-Resistant Escherichia coli Strain upon Meropenem ExposureJournal of Proteome ResearchAugust 7, 2026Leonarda Acha Alarcon, Daniel Jaén-Luchoro, Francisco Salvà-Serra, Edward R. B. Moore, Ivan Mijakovic, Roger Karlssonbeads were resuspended in 50 mM TEAB, and proteins were digested with a Trypsin/Lys-C mix (1:25; ref. V5071; Promega; WI; USA) for 2 h, followed by trypsin digestion (1:50; ref. V5111; Promega; WI; USA) overnight.

(14) DCAF11-dependent molecular glue degrader activated by glutathionylationNatureAugust 5, 2026Hojong Yoon, Franziska Wachter, Katharine A. Barrett, Cyrus Jin, Anna Rodríguez-Pöhnlein, Justine C. Rutter, Ryan J. Lumpkin, Rebecca J. Metivier, Katherine A. Donovan, Kheewoong Baek, et al.mM). Subsequently, proteins were digested with 3 µg of Trypsin/Lys-C Mix, Mass Spec Grade (Promega V5072) overnight at 37 °C. Sample digests were acidified with formic acid to a pH of 2–3 prior to desalting

(15) Microcephaly-like phenotype triggered by novel reassortant and prototypic Oropouche virus strains in brain organoidsEBioMedicineJuly 30, 2026Gabrielle Brum, Vivian Grizente, Fábio Luís Lima Monteiro, Beatriz Luzia de Mello Lima Guimarães, Livia Goto-Silva, João Marcos de Azevedo Delou, Pedro Junior Pinheiro Mourão, Ismael Carlos da Silva Gomes, Enzo Oliveira Barone, Matheus Villanueva Andrade, et al.undesired surfactants followed by on-bead proteolysis (37 °C, overnight) with Trypsin/Lys-C (Promega, V5071) (enzyme/substrate ratio = 1:25). Following proteolysis, peptides were quantified using the Pierce Quantitative Fluorometric Peptide Assay

(16) Enrichment-free deep proteomics enables proteome-scale analysis of methionine oxidationDNA ResJuly 27, 2026Hiromasa Mitsui, Yusei Okuda, Ryo Konno, Daisuke Nakajima, Osamu Ohara, Yusuke Kawashimaneopentyl glycol with or without 10 mM methionine, followed by addition of 1 μg of trypsin/Lys-C Mix (CAT# V5072, Promega, Madison, WI, USA), and mixed gently at 37 °C 16 h to digest proteins. The digested peptides

(17) Time-Resolved Label-Free Proteomics of SHK-1 Cells After Renibacterium salmoninarum Inoculation Reveals Early Host-Cell RemodelingInternational Journal of Molecular SciencesJune 26, 2026Jorge F. Beltrán, Jörn Bethke, Sandra Flores-Martin, Claudia A. Barrientos, Marcelo Aguilar, Adolfo Isla, Felipe Almendras, Marcos Mancilla, Alejandro J. Yañezwith 25 mM ammonium bicarbonate. Proteolytic digestion was carried out with sequencing-grade trypsin (#V5071, Promega, Madison, WI, USA) at a 1:50 protease-to-protein ratio (w/w) for 16 h at 37 °C. Digestion was

(18) A Micro-Quantitative and FFPE-Compatible Workflow for Immunohistochemistry-Guided Spatial Proteomic Analysis of Cellular Subpopulations Within the Tumor MicroenvironmentBioengineering (Basel)June 11, 2026Junya Peng, Lu Ping, Ruikang Dun, Lulu Liu, Yihong Shi, Ruizhe He, Qing Zhong, Yang Chen, Wenmin Tian, Yupei ZhaoProtein digestion under low-input conditions For proteolytic digestion, approximately 500 ng of Trypsin/Lys-C Mix (V5071, Promega, Madison, WI, USA) was added to each sample. Samples were incubated at 37 °C for 16–18 h with

(19) A proteogenomic RNA processing mechanism drives sex differences in meningiomaResearchSquareJune 2, 2026David Raleigh, Martha Cady, Ayush Aggarwal, Nicholas Stevers, Naomi Zakimi, Isabelle Liu, Kanish Mirchia, John Coukos, Pornparn Kongpracha, Yuan Zhou, et al.three times with 200μl acetonitrile. Rinsed beads were reconstituted in 10μL of 25mM Tryspin/Lys-C (V5071, Promega) in 50μM of ammonium bicarbonate. Samples were incubated at 37°C overnight. The following day,

(20) A comprehensive approach to elucidating the pathophysiology of kidney fibrosis based on extracellular vesicle proteomicsFrontiers in PhysiologyMay 29, 2026Yaerim Kim, Kyuhyeon Kim, Hong-Beom Park, Sunwha Lee, Mi-yeon Yu, Young Joo Kim, Soo Bin Choi, Woo Yeong Park, Kyubok Jin, Dong Ki Kim, et al.bicarbonate, protein digestion was performed overnight at 37 °C using trypsin/Lys-C Mix (Promega; Cat# V5071) at a 100:1 protein-to-protease ratio. All resulting peptides were acidified with 10% trifluoroacetic

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Trypsin/Lys-C Mix, Mass Spec Grade

Trypsin/Lys-C Mix, Mass Spec Grade from Promega

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Trypsin/Lys-C Mix, Mass Spec Grade from Promega

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