Q5 Hot Start High-Fidelity DNA Polymerase M0493S from New England Biolabs

Product Specs
ItemQ5 Hot Start High-Fidelity DNA Polymerase
CompanyNew England Biolabs
Price $151.00 Inquire
Catalog NumberM0493S
Size100 units
ApplicationsDNA Analysis
TypeDNA Polymerase
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Engineering novel AAV capsids by broadly attenuated and subsequent muscle-specific tropism in mice and NHPsMolecular Therapy AdvancesJune 11, 2026Yue Pan, Yujian Zhong, Huan Chen, Youwei Zhang, Zhiyong Dai, Junlin Chen, Keqin Tan, Xiaoqu Chen, Danlan Qiu, Longxiang Sheng, et al.R312-02). PCR was performed with barcoded primers and the Q5 Hot Start high-fidelity DNA polymerase (NEB, M0493L). PCR amplicons were purified by Hieff NGS DNA Selection Beads (Yeason, 12601ES08) and sequenced using

(2) Multi-site DMS probing reveals higher-order structure of RNA-protein complexes in living cellsMol CellApril 21, 2026Irfana Saleem, Thomas Miller, Lucas Kearns, Anthony Hoang, Joshua Meehan, Ritwika Bose, David Mitchell, David H. Price, Calla M. Olson, Chase A. Weidmann, et al.AM2238 Superscript II Reverse Transcriptase,Invitrogen,Cat# 18064014 Q5 Hot Start Polymerase,NEB,Cat# M0493L TEV Protease,Takara,Cat# 635668 Pierce Glutathione Agarose,Thermo Scientific,Cat# 16100 TALON Metal

(3) Enzymatically Controlled Release of Proteins and Peptides (ENCOREP): A Promising Alternative Secretion ApproachiScienceApril 17, 2026Daniel Ivanusic, Gregor Zipf, Josef Maier, Hubert Bernauer, Norbert Bannert,New England Biolabs,Cat# R3101 EcoRV-HF,New England Biolabs,Cat# R3195 Q5 Hotstart polymerase,New England Biolabs,Cat# M0493 DpnI,New England Biolabs,Cat# R0176S PmeI,New England Biolabs,Cat# R0560 NheI-HF,New England Biolabs,Cat#

(4) Intramolecular competition generates pulsatory protein activity shaped by light, temperature, and evolutionbioRxivApril 16, 2026Zikang (Dennis) Huang, Malvin Forson, William Benman, Kevin H. Gardner, Lukasz J. Bugajprimers with altered sequences. DNA templates were amplified using Q5 hot start polymerase from NEB (M0493S), followed by HiFi assembly (NEB E2621S) with backbone DNA digested with proper restriction enzymes.

(5) Phage-assisted evolution of allosteric protein switchesNat CommunApril 14, 2026Nicholas T. Southern, Anna von Bachmann, Alisa Hovsepyan, Marielouise Griebl, Benedict Wolf, Nina Lemmen, Ann-Sophie Kroell, Simon Westermann, Jan Mathony, Dominik Niopekfragments were amplified using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs, cat. no. M0493S). PCR products were gel-purified and assembled using NEB Type IIs restriction enzymes and T4 DNA Ligase

(6) Systematic CRISPRi screening reveals genetic modulators of E. coli isoprenoid productionbioRxivApril 10, 2026Dhiraj Dokwal, Philip M. Brown, Christine Ingle, Scott H. Saunders, Kimberly A. Reynoldssequences were appended to the sgRNA cassette using Q5 Hot Start High-Fidelity DNA Polymerase (NEB, M0493) with adapter-specific primers. For plate-level identification in the large-scale genomic screen, an

(7) Notes on the life cycle of a new marine ciliate, Metafolliculina songi n. sp. (Protozoa, Ciliophora, Heterotrichea), with description of its infraciliature and phylogenyMarine Life Science & TechnologyApril 7, 2026Tingting Ye, Mingzhen Ma, Jemin Park, Mann Kyoon Shin, Yaohan Jiang, Wen Song, Lifang Li, Yong Chi, Xiangrui Chenpossibility of PCR amplification errors, Q5 Hot Start High-Fidelity DNA Polymerase (NEB Co., Ltd., M0493, Beijing, China) was employed. PCR products were checked using agarose gel and sequenced by TSINGKE

(8) Profiling KRAS mutations in whole blood by error-corrected maximum depth sequencingnpj Precision OncologyApril 3, 2026Ryne C. Ramaker, Yuchen Zhang, John H. Strickler, Peter Allen, James Abbruzzese, Christopher M. Counterthe eluant (33 µL) was mixed with 0.5 µL of Q5 Hot Start High-Fidelity Polymerase (NEB Catalogue # M0493L), 10 µL of Q5 buffer, 4 µL of 2.5 mM dNTP mix (N0447L), and 2.5 µL of 10 µM barcoding primer encoding

(9) Synthetic circRNAs employ IRES activity for translation in cells and in cell-free translation systemsbioRxivMarch 30, 2026Philipp Koch, Frederic S. W. Arendrup, Chaehee Lim, Samyukta Narayanan, Amina Adam, Massimiliano Clamer, Anders H. Lund, Chun-Kan Chen, Kathrin Leppekoverhangs using primers PK_352–PK_391 according to manufacturer’s recommendations (NEB Q5 Polymerase, M0493S). The plasmid backbone was digested using EcoRI-HF (NEB, R3101S) and BsiWI-HF (NEB, R3553S) restriction

(10) SMARCA2 is an essential and potent cofactor for a specific subset of the glucocorticoid response in A549 cellsbioRxivMarch 25, 2026Schuyler M Melore, D Dewran Koçak, Keith Siklenkla, Graham D Johnson, Courtney Williams, Luke C Bartelt, Angela G Jones, Alejandro Barrera, Revathy Venukuttan, Charles A Gersbach, et al.8.3 μg of gDNA was used as template across 8 100 μL PCR reactions using Q5 hot start polymerase (NEB, M0493L). Amplification was carried out following the manufacturer’s instructions using 25 cycles at an annealing

(11) APC coordinates GSK3 phosphorylation of SETD8 to suppress colorectal cancerCell ReportsMarch 24, 2026Zvi Cramer, Keara Monaghan, Ricardo Petroni, Xin Wang, Stephanie Adams-Tzivelekidis, Kayla Durning, Melissa S. Kim, Yuhua Tian, Nicolette M. Johnson, María F. Carrera Rodríguez, et al.manufacturer’s instructions (Agilent #210518). Human SETD8 cDNA was cloned with Q5 polymerase (NEB #M0493S) using the following primers: SETD8-cDNA-F: 5′-CGCTCTAGAATGGCTAGAGGCAGGAAGATGTGC-3’; SETD8-cDNA-R: 5′-CGCGGATCCCCACGTGGTTAG

(12) Lysosomal down-regulation of the mu opioid receptor is opposed by the Retromer complexSci AdvMarch 20, 2026Aleksandra Dagunts, Hayden Adoff, Brandon Novy, Lamya Ben Ameur, Monica De Maria, Arpiar Saunders, Braden T. Lobingier(QIAGEN, 51104). sgRNA libraries were prepared using Q5 Hot Start High-Fidelity DNA Polymerase (NEB, M0493L) and barcoding primers. PCR products were purified using QIAquick PCR purification columns (QIAGEN,

(13) Integrated Size-Selective Cell Purification and Electroporation for Genetic Manipulation of Primary CellsMicromachines (Basel)March 15, 2026Hyun Woo Sung, Soojung Claire Hurintroduced via site-directed mutagenesis using the Q5 Hot Start High-Fidelity DNA Polymerase Kit (cat# M0493S, New England Biolabs, Ipswich, MA, USA), with the following primers: forward: 5′- ACT CAC TAT AAG GAG

(14) HIV broadly neutralizing antibody escape dynamics drive the outcome of AAV-vectored immunotherapy in humanized miceImmunityMarch 10, 2026Nicolas M.S. Galvez, Adam D. Nitido, Seo Bin Yoo, Yi Cao, Cailin E. Deal, Christine L. Boutros, Scott W. MacDonald, Yentli E. Soto Soto Albrecht, Evan C. Lam, Maegan L. Sheehan, et al.0.5 μM of strain specific primers and 0.02U/μL of Q5 Hot Start High-Fidelity DNA Polymerase (NEB, Cat#M0493L) in a total reaction volume of 25 μL. PCR conditions for initial round were: 98°C for 30s, followed

(15) Genomic characterization of dengue virus in the Ningxia Hui Autonomous Region, China (2019 and 2023)Genome Biology and EvolutionMarch 2, 2026Longshan Li, Jun Zhan, Dongzhi Yang, Tao Li, Xiaoqiang Sun, Fang Yuan, Min Cao, Wei Zhang, Ting Mu, Jingting Wang, et al.primers (Table S2) and Q5 Hot Start High-Fidelity DNA Polymerase (New England BioLabs, catalog no. M0493L) to ensure amplification accuracy. PCR products were purified using AMPure XP beads (Beckman Coulter,

(16) In vivo CRISPR/Cas9 screens identify new regulators of B cell activation and plasma cell differentiationJournal of Experimental MedicineMarch 2, 2026Lesly Calderón, Markus Schäfer, Marina Rončević, René Rauschmeier, Markus Jaritz, Tanja A. Schwickert, Qiong Sun, Andrea Pauli, Johannes Zuber, Meinrad Busslingerusing a two-step PCR protocol. The first PCR used 0.5 μl of Q5 Hot Start High-Fidelity DNA Polymerase (M0493; New England Biolabs) in 50-μl reactions containing 100 ng of genomic DNA. For each sample, the resulting

(17) Identification of a cytosol-to-nucleus feedback loop that regulates neuronal microtubule nucleationJournal of Cell BiologyMarch 2, 2026Nitish Kumar, Nathaniel Carey, J. Ian Hertzler, Annabelle R. Bernard, Gibarni Mahata, Gregory O. Kothe, Yitao Shen, Melissa M. Rollsrestriction enzymes were from NEB and thermostable DNA polymerase Q5 was from NEB (Q5 hotstart NEB M0493S). The generation of pUAS-Kat60-mScarlet3 constructs The amino-terminus of Kat60 was PCR amplified with

(18) CRAGE-RB-PI-seq reveals transcriptional dynamics of plant-associated bacteria during root colonizationNature CommunicationsFebruary 21, 2026Tomoya Honda, Sora Yu, Dung Mai, Leo Baumgart, Emory M. Chan, Gyorgy Babnigg, Yasuo Yoshikuniperformed for 7 cycles in a total volume of 50 µl using Q5® Hot Start High-Fidelity DNA Polymerase (NEB: M0493L). The amplified products were subsequently gel-purified. For the conjugal vector, we used a modified

(19) Evidence that extra copies of chromosome 1q play a role in the early phases of pancreatic neoplasiaScience AdvancesFebruary 20, 2026Christopher Douville, Jeeun Parksong, Marco Dal Molin, Sarah Graham, Patricia T. Greipp, Ryan Knudson, Samuel Curtis, Yuxuan Wang, Lisa Dobbyn, Maria Popoli, et al.supplemented with an additional 0.5 U of Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs, M0493L), 1 μl of 10 mM deoxynucleoside triphosphates (Deoxynucleoside triphosphates; New England Biolabs, N0447L)

(20) Allelic Variation at 9p21.3 Orchestrates Widespread RNA Splicing Shifts Governing Vascular Smooth Muscle Cell PlasticitybioRxivFebruary 11, 2026S. Suryavanshi, H. Yang, E. Salido, V. Lo Sardo(5′ GACTTTCCCCCA CAATGAAA 3′). Genotyping PCR was performed using Q5 Hot Start DNA polymerase (NEB M0493S) in 25uL reactions. Reaction parameters include: 30 cycles, 58 °C annealing, 30sec extension, 200ng

Write a Review

(1)

4/9/2015Hi Fidelity Polymerase Benjamin GerovacI used this polymerase while performing PCR to subclone a gene fragment into a viral vector so I could transduce primary epithelial cells. Upon sequencing, this polymerase gave a PCR product of the ... Read Review

Q5 Hot Start High-Fidelity DNA Polymerase

Q5 Hot Start High-Fidelity DNA Polymerase from New England Biolabs

New England Biolabs

Citations (326)

Reviews

Q5 Hot Start High-Fidelity DNA Polymerase from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map