ProtoScript II Reverse Transcriptase M0368X from New England Biolabs

Product Specs
ItemProtoScript II Reverse Transcriptase
CompanyNew England Biolabs
Price $706.00 Inquire
Catalog NumberM0368X
Size40000 units
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) PeakPrime: a peak-guided primer design pipeline for target enrichment in 3′-end RNA-seqBioinform AdvMarch 19, 2026Franco Poma-Soto, Brecht Soulliaert, Hanne Van Droogenbroeck, Pieter Mestdagh, Jo VandesompelecDNA was synthesized in 100 μL using 30 nM oligo(dT)-VN capture probe, 1× Protoscript RT buffer (NEB; M0368X), 10 ng/μL RNA, 0.01 M DTT (NEB; M0368X), 0.5 mM dNTPs, 10 U/μL Protoscript RT (NEB; M0368X), 0.4 U/μL

(2) FFAR4-mediated IL-6 release from islet macrophages promotes insulin secretion and is compromised in type-2 diabetesNature communicationsApril 10, 2025Chen X, Shao J, Brandenburger I, Qian W, Hahnefeld L, Bonnavion R, Cho H, Wang S, Hidalgo J, Wettschureck N, et al.Complementary DNA synthesis was performed using the ProtoScript II Reverse Transcription kit (NEB, M0368X). Quantitative real-time PCR was performed with the SYBR Green PCR Master System (Thermo Fischer, 4368708).

(3) Deubiquitinating enzyme mutagenesis screens identify a USP43-dependent HIF-1 transcriptional responseThe EMBO JournalJuly 15, 2024Tekle Pauzaite, Niek Wit, Rachel V Seear, James A NathanMinikit,Qiagen,74134 PureLink RNA Mini Kit,Thermo Scientific,12183025 Protoscript II Reverse Transcriptase,NEB,M0368X Protein G magnetic beads,Thermo Scientific,88848 EDTA-free protease inhibitor cocktail tablet,Roche,11873580001 "XF

(4) Quantification of elongation stalls and impact on gene expression in yeastRNADecember 1, 2023Wanfu Hou, Vince Harjono, Alex T. Harvey, Arvind Rasi Subramaniam, Brian M. Zidamounts of RNA from each sample using Protoscript II Reverse Transcriptase (New England Biolabs cat. no. M0368X) and an oligo dT(18) primer according to the manufacturer's instructions. RT-qPCR was done using a home-brew

(5) Hierarchical Phosphorylation of HOXB13 by mTOR Dictates Its Activity and Oncogenic Function in Prostate CancerMolecular Cancer ResearchOctober 2, 2023Yonghong Chen, Catherine R. Dufour, Lingwei Han, Ting Li, Hui Xia, Vincent Giguère74106). RNA (1 μg) was reverse transcribed using ProtoScript II Reverse Transcriptase (NEB, catalog no. M0368X) and measured by quantitative real-time PCR using SYBR Green Master Mix (Roche, catalog no. 4887352001)

(6) Quantification of elongation stalls and impact on gene expression in yeastbioRxivMarch 24, 2023Wanfu Hou, Vince Harjono, Alex T. Harvey, Arvind Rasi Subramaniam, Brian M. Zidamounts of RNA from each sample using Protoscript II Reverse Transcriptase (New England Biolabs Cat. No. M0368X) and an oligo dT(18) primer according to the manufacturer’s instructions. RT-qPCR was done using a homebrew

(7) Expanding luciferase reporter systems for cell-free protein expressionScientific ReportsDecember 1, 2022Wakana Sato, Melanie Rasmussen, Christopher Deich, Aaron E. Engelhart, Katarzyna P. AdamalaGATCCCGGCGGC), 10 mM DTT, 0.5 mM dNTP (Denville, CB4430-2), 5 U/μl protoscript II reverse transcriptase (NEB, M0368X), 1 × protoscript II reverse transcriptase buffer, and 0.4 U/μl Murine RNase Inhibitor (NEB, M0314S).

(8) PepSeq: a fully in vitro platform for highly multiplexed serology using customizable DNA-barcoded peptide librariesNature ProtocolsNovember 16, 2022Sierra N. Henson, Evan A. Elko, Piotr M. Swiderski, Yong Liang, Anna L. Engelbrektson, Alejandra Piña, Annalee S. Boyle, Zane Fink, Salvatore J. Facista, Vidal Martinez, et al.conversion (RDC) specific reagents ProtoScript II reverse transcriptase (New England Biolabs, cat. no. M0368X) RNase H (New England Biolabs, cat. no. M02971) Protease inhibitor cocktail (VWR, cat. no. G6521)

(9) A ubiquitous amino acid source for prokaryotic and eukaryotic cell-free transcription-translation systemsFrontiers in Bioengineering and BiotechnologySeptember 16, 2022Lakshmeesha K. Nagappa, Wakana Sato, Farzana Alam, Kameshwari Chengan, Christopher M. Smales, Tobias Von Der Haar, Karen M. Polizzi, Katarzyna P. Adamala, Simon J. Moore(GATCCCGGCGGC), 10 mM DTT, 0.5 mM dNTP (Denville, CB4430-2), 5 U/µl protoscript II reverse transcriptase (NEB, M0368X), 1x protoscript II reverse transcriptase buffer, and 0.4 U/µL Murine RNase Inhibitor (NEB, M0314S).

(10) Rvb1/Rvb2 proteins couple transcription and translation during glucose starvationeLifeSeptember 15, 2022Yang S Chen, Wanfu Hou, Sharon Tracy, Alex T Harvey, Vince Harjono, Fan Xu, James J Moresco, John R Yates, Brian M ZidRNA Purification Kit (Epicentre). cDNA was prepared using ProtoScript II Reverse Transcriptase (NEB #M0368X) with a 1:1 combination of oligodT 18 primers and random hexamers (NEB) according to the manufacturer’s

(11) COVID-19 vaccination elicits an evolving, cross-reactive antibody response to epitopes conserved with endemic coronavirus spike proteinsCell ReportsJuly 5, 2022Evan A. Elko et al."RNase Inhibitor, Murine",New England Biolabs,Cat# M0314L ProtoScript II Reverse Transcriptase,New England Biolabs,Cat# M0368X RNase H,New England Biolabs,Cat# M02971 Q5 High-Fidelity DNA Polymerase,New England Biolabs,Cat# M0491L T4

(12) Insulin action and resistance are dependent on a GSK3β-FBXW7-ERRα transcriptional axisNature communicationsApril 19, 2022Xia H, Scholtes C, Dufour CR, Ouellet C, Ghahremani M, Giguère V.respectively. RNA was then reverse transcribed using ProtoScript II Reverse Transcriptase (cat. no. M0368X; NEB) according to manufacturer’s instructions. Synthesized cDNA was amplified by quantitative real-time PCR

(13) Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptationeLifeApril 9, 2021Lamers MM, Mykytyn AZ, Breugem TI, Wang Y, Wu DC, Riesebosch S, van den Doel PB, Schipper D, Bestebroer T, Wu NC, et al.4337456, Commercial assay or kit,ProtoScript II Reverse Transcriptase,New England BioLabs,Cat# NEB M0368X, Commercial assay or kit,KAPA HyperPlus,Roche,Cat# 7962428001, Other,Amicon Ultra-15 Centrifugal Filter

(14) Epitope-resolved profiling of the SARS-CoV-2 antibody response identifies cross-reactivity with endemic human coronavirusesCell Reports MedicineJanuary 19, 2021Jason T. Ladner, Sierra N. Henson, Annalee S. Boyle, Anna L. Engelbrektson, Zane W. Fink, Fatima Rahee, Jonathan D’ambrozio, Kurt E. Schaecher, Mars Stone, Wenjuan Dong, et al."RNase Inhibitor, Murine",New England Biolabs,Cat# M0314L ProtoScript II Reverse Transcriptase,New England Biolabs,Cat# M0368X RNase H,New England Biolabs,Cat# M02971 Q5 High-Fidelity DNA Polymerase,New England Biolabs,Cat# M0491L T4

(15) Mitochondrial volume fraction and translation duration impact mitochondrial mRNA localization and protein synthesiseLifeAugust 7, 2020Tatsuhisa Tsuboi et al.RNA Purification Kit (Epicentre). cDNA was prepared using ProtoScript II Reverse Transcriptase (NEB #M0368X) with a combination of oligo(dT) primers and random hexamers according to the manufacturer’s instructions.

(16) Nucleoporin 107, 62 and 153 mediate Kcnq1ot1 imprinted domain regulation in extraembryonic endoderm stem cellsNature CommunicationsDecember 1, 2018Saqib S. Sachani, Lauren S. Landschoot, Liyue Zhang, Carlee R. White, William A. MacDonald, Michael C. Golding, Mellissa R. W. Mann18068015) treatment as described1. cDNA was prepared using ProtoScript® II Reverse Transcriptase (NEB, M0368X) as per instructions with oligodT23 (Sigma, O4387) and Random Primers (Life Technologies, 48190011).

(17) A novel environment-evoked transcriptional signature predicts reactivity in single dentate granule neuronsNature communicationsAugust 6, 2018Jaeger BN, Linker SB, Parylak SL, Barron JJ, Gallina IS, Saavedra CD, Fitzpatrick C, Lim CK, Schafer ST, Lacar B, et al.buffer were processed as follows: (1) Reverse transcription with ProtoScript II (New England Biolabs, #M0368X), (2) PCR preamplification with 2 × KAPA Hifi HotStart ReadyMix (Kapa, #KK2602), (3) Bead clean up with

(18) Multi-omics study revealing the complexity and spatial heterogeneity of tumor-infiltrating lymphocytes in primary liver carcinomaOncotargetMay 23, 2017Lijun Shi, Yang Zhang, Lin Feng, Liming Wang, Weiqi Rong, Fan Wu, Jianxiong Wu, Kaitai Zhang, Shujun Chengassay. Primer TRBC Ro (Supplementary Table 7) and ProtoScript II Reverse Transcriptase (NEB, cat. no. M0368X) were used to synthesize TCRB-specific cDNA from 500 ng of total RNA. For the first PCR reaction, pre-amplification

(19) Organ-specific alteration in caspase expression and STK3 proteolysis during the aging processNeurobiology of AgingNovember 1, 2016Mélissa Lessard-Beaudoin, Mélissa Laroche, Amal Loudghi, Marie-Josée Demers, Jean-Bernard Denault, Guillaume Grenier, Sean-Patrick Riechers, Erich E. Wanker, Rona K. Grahamwith the RNeasy mini kit (QIAGEN) and cDNAs were prepared using ProtoScript Reverse Transcriptase II (#M0368X, New England BioLabs). Quantification was done using Mx3005P QPCR Systems (Stratagene) with mouse-specific

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ProtoScript II Reverse Transcriptase

ProtoScript II Reverse Transcriptase from New England Biolabs

New England Biolabs

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ProtoScript II Reverse Transcriptase from New England Biolabs

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