Q5 High-Fidelity 2X Master Mix M0492S from New England Biolabs

Product Specs
ItemQ5 High-Fidelity 2X Master Mix
CompanyNew England Biolabs
Price $200.00 Inquire
Catalog NumberM0492S
Size100 reactions
ApplicationsDNA Analysis
TypeMaster Mix
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Cockayne syndrome mutation in XPG activate the integrated stress responseHuman GeneticsDecember 1, 2026Danhui Zhang, Max Hartmann, Zhouli Cao, Gaojie Zhu, Gregoire Najjar, Cagatay Günes, Steffen Emmert, Karin Scharffetter-Kochanek, Sebastian Ibenextraction and cDNA synthesis, DNA products were amplified by using Q5 high-fidelity 2 × master mix (M0492S, NEB). Briefly, DNA products were mixed with Q5 high-fidelity 2 × master mix (M0492S, NEB), 10 μM forward

(2) Rhythmic protein condensation in the circadian regulation of glycogen metabolismiScienceMay 15, 2026Lily A. Burton, Gopal K. Pattanayak, Michael J. Rustproteins","Chemicals, peptides, and recombinant proteins" ,, Q5 High-Fidelity 2X Master Mix,New England Biolabs,Cat# M0492S EcoRI-HF,New England Biolabs,Cat# R3101S Gibson Assembly Master Mix,New England Biolabs,Cat# E2611S "cOmplete,

(3) ISGylation is disrupted by UBA7 gene variants identified in individuals with neurodevelopmental disorder phenotypesiScienceMay 15, 2026Venkateshwarlu Bandi, Myrrhe Venema, Iona Wallace, Merel O. Mol, Anita Nikoncuk, Rachel Schot, Marjon van Slegtenhorst, Emilia K. Bijlsma, Amjad Khan, Susan M. White, et al.RR820L In-Fusion Master Mix,Takara Bio,Cat# 638947 Q5 High-Fidelity 2X Master Mix,New England Biolabs,Cat# M0492L Pierce BCA Protein assay kit,Thermo Fisher Scientific,Cat# 23225 ,, Deposited data,Deposited data,Deposited

(4) Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducensNucleic Acids ResApril 24, 2026Benjamin F Jepson, Eric J Wolf, Anthony R Dawson, Jingmin Jin, Vladimir Potapov, Nan Dai, Ivan R Corrêa Jr., Erbay Yigitpolymerase chain reaction (PCR) using forward and reverse T7 primers using Q5 PCR master mix (NEB, M0492) and purified (NEB, T1130S). The template DNA was in vitro transcribed using T7 RNA polymerase (NEB,

(5) Polarized SOS activity orchestrates FGF-directed cell migration in vivoCurrent BiologyApril 20, 2026Theresa V. Gibney, Laila Y. Latifi, Jacob I. Mardick, Neal R. Rasmussen, Maria C. Lyons, Meera V. Sundaram, David J. Reiner, Ariel M. PaniPureLink HQ Mini Plasmid DNA Purification Kit,Invitrogen,K210001 Q5 High-Fidelity 2X Master Mix,New England Biolabs,M0492L Q5 Site-Directed Mutagenesis Kit,New England Biolabs,E0554S Key Resources Table

(6) PALINCODE: Recording cell lineage with ternary palindromic CRISPR bitsbioRxivApril 19, 2026Maryam Fathi, Aidan Cook, Bagheri Bagheri, Tyler Curiel, Aaron McKennaKit (#M2200) were used, all sourced from New England Biolabs (NEB). Q5® High-Fidelity 2X Master Mix (#M0492) from NEB was used for PCR amplification. All PCR products and DNA fragments were purified using the

(7) 2'-O-Methylation maintains ribosome structural and translation integrityMol CellApril 16, 2026Yu Zhao, Jay Rai, Lauren N. Cohen, Yuerong Shu, Chong Xu, Hemant Goswami, Xin Chen, Hong Jin, Virginie Marchand, Yuri Motorin, et al.BIO-TEK,D6942-01 E.Z.N.A.® Gel Extraction Kit,Omega BIO-TEK,D2500-01 Q5® High-Fidelity 2X Master Mix,NEB,M0492S HiScribe® T7 mRNA Kit with CleanCap® Reagent AG,NEB,E2080S Monarch® Spin RNA Cleanup Kit,NEB,T2050L The

(8) Endogenous protein tagging coupled with a CRISPR screening approach identifies UBE3C as a potential MYC oncogene regulatorSci RepApril 11, 2026Marcel Seibert, Nina Kurrle, Sifora Kaleab, Frank Wempe, Ivana von Metzler, Hubert Serve, Frank SchnütgensgRNA-encoding regions was performed using the Q5 High-Fidelity 2X Master Mix (New England Biolabs, Germany, #M0492L). Up to 80 parallel PCRs were performed per sample to ensure sufficient amplification, using staggered

(9) Intragenic Transcription from Defective HIV Proviruses Triggers Interferon Responses in Myeloid CellsbioRxivApril 7, 2026Jonathan Kilroy, Aparna Deokar, Samantha Patalano, Juan Fuxman Bass, Manish Sagar, Suryaram Gummuluru, Andrew J. Hendersonand 1.25μL of each primer was added to 25μL of Q5 2X master mix (New England Biolabs, catalog number M0492S) and the total volume adjusted to 50μL. Samples were amplified for 30–45 cycles with an annealing temperature

(10) Paired genomic and proteomic analysis of Acinetobacter towneri from waste sludge reveals an extensive repertoire of enzymes for breakdown of hydrocarbonsMicrobiology SpectrumApril 7, 2026Ronja Marlonsdotter Sandholm, Dave Rojas Calderón, Ørjan Dahl, Ravindra Reddy Chowreddy, Gustav Vaaje-Kolstad, Sabina Leanti La Rosa5′-GGTTACCTTGTTACGACTT-3′), 25 µL Q5 High-Fidelity 2X Master Mix (New England Biolabs, cat. number: M0492S), and 18 µL dH2O. The 16S rRNA gene was amplified using the following program: initial denaturation

(11) Synthetic budding morphogenesis by optogenetic receptor tyrosine kinase signalingbioRxivApril 2, 2026Louis S. Prahl, Ronald Canlla, Aria Zheyuan Huang, Daniel S. Alber, Sandra L. Shefter, Sachin N. Davis, Samuel H. Grindel, Zikang Dennis Huang, Thomas R. Mumford, William Benman, et al.design). Cloning: Linear DNA fragments were produced by polymerase chain reaction (PCR) using Q5 polymerase (#M0492S, New England Biolabs) with cycle times and temperatures adjusted according to manufacturer instructions.

(12) HIV Promoters Isolated from Brain and Peripheral Tissue of Virally Suppressed PWH Are Phylogenetically and Functionally SimilarInternational Journal of Molecular SciencesMarch 31, 2026Janna Jamal Eddine, Emily K. Chalmers, Jingling Zhou, Sarah J. Byrnes, Trisha A. Jenkins, Narin Osman, Anna C. Hearps, Michael Roche, Thomas A. Angelovich, Melissa J. ChurchillA 25 µL reaction was assembled using 12.5 µL Q5 High-Fidelity 2X Master Mix (NEB, Ipswich, MA, USA; M0492L), 1 µL of 10µM forward primer, 1 µL of 10 µM reverse primer, 1 µL of template DNA (up to 150 ng/reaction)

(13) A self-complementary recombinant adeno-associated virus vector coding for an anchorless prion protein carrying the G127V mutation extends survival in a rodent prion disease modelbioRxivMarch 30, 2026Thomas Zerbes, Claire Verkuyl, Cunjie Zhang, Sophie Grunnesjoe, Shehab Eid, Hamza Arshad, Wenda Zhao, Zahra Nasser, Teaghan O’Shea, Ari Belotserkovsky, et al.codon, was undertaken with a PCR reaction mix composed of Q5 High-Fidelity 2x Master Mix (catalog number M0492S, New England Biolabs, Ipswitch, MA, USA), template DNA and the following primers: Forward: 5’– tcaggttggaccggctagcaccggtgccaccATGGCCAACCTcagctactg

(14) SAMHD1 depletion restricts SARS-CoV-2 infection by suppressing HNF1-dependent ACE2 expression in lung epithelial cellsPLoS PathogMarch 19, 2026Pak-Hin Hinson Cheung, Pearl Chan, Hua Yang, Krisztina Ambrus, Shravya Honne, Baek Kim, Stanley Perlman, Li Wugenerated through overlapping PCR as mentioned before using Q5 High-Fidelity 2X Master Mix (NEB, Cat. no. M0492S) [97]. The sequences of the primers used in the first and second round PCR were listed in S5 Table.

(15) In vivo site-specific engineering to reprogram T cellsNatureMarch 18, 2026William A. Nyberg, Pierre-Louis Bernard, Wayne Ngo, Charlotte H. Wang, Jonathan Ark, Allison Rothrock, Gina M. Borgo, Gabriella R. Kimmerly, Jae Hyung Jung, Vincent Allain, et al.performed with defined primer sets (Supplementary Table 1) for 18 cycles using Q5 polymerase (NEB, M0492S), and amplicons were PCR-purified (IBI Scientific, IB47010). In round 2, indices for demultiplexing

(16) The E3 ubiquitin ligase mechanism specifying targeted microRNA degradationNatureMarch 18, 2026Jakob Farnung, Elena Slobodyanyuk, Peter Y. Wang, Lianne W. Blodgett, Daniel H. Lin, Susanne von Gronau, Brenda A. Schulman, David P. Bartelsite-directed mutagenesis by direct PCR with the KAPA HiFi HotStart Ready Mix (Roche, 07958935001) or Q5 Master Mix (NEB, M0492S). Plasmids were purified using the Plasmid Plus Midi Kit (QIAGEN, 12945) and verified by Sanger or nanopore sequencing

(17) Comparison of state-of-the-art error-correction coding for sequence-based DNA data storageNature CommunicationsMarch 14, 2026Andreas L. Gimpel, Alex Remschak, Wendelin J. Stark, Reinhard Heckel, Robert N. Grassoligo pool was then amplified with 10 µL Q5 High-Fidelity polymerase master mix (New England Biolabs, M0492S), 1 µL of 10 µM 0 F and 0 R primers each (Microsynth, Balgach, Switzerland, see Supplementary Table

(18) Multi-omic profiling of human and mouse dorsal root ganglia enables targeted gene delivery to nociceptorsbioRxivMarch 14, 2026Lily S. He, Parth Bhatia, Shamsuddin A. Bhuiyan, Evangelia Semizoglou, Jiaxiang Wang, Jia Li, Joohyun Nam, Jay X. J. Luo, Chayse Arnhold, Difei Zhu, et al.or as synthetic gBlocks (IDT). Templates were amplified using Q5 High-Fidelity DNA Polymerase (NEB M0492S) under standard cycling conditions, verified by agarose gel electrophoresis, and purified (QIAquick,

(19) Nationwide wastewater sequencing surveillance of SARS-CoV-2 lineages: validation against clinical data across 28 U.S. statesPeerJMarch 13, 2026Alessandro Zulli, Bradley White, Dorothea Duong, Bridgette Shelden, Vikram Chan-Herur, Marlene Wolfe, Alexandria B. Boehmusing 2X HyperPure beads and added to a 12.5 µL PCR using the NEB Q5 High-Fidelity 2X Master Mix (NEB M0492) and ARTIC primers (pool A or pool B) and cycled according to the ARTIC PCR protocol as optimized by

(20) Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosisNat Struct Mol BiolMarch 13, 2026Kirandeep K. Deol, Cynthia A. Harris, Sydney J. Tomlinson, Colin J. Delaney, Amr Al-Farhan, Alyssa J. Mathiowetz, Cody E. Doubravsky, Derek A. Pratt, James A. Olzmannon an Applied Biosystems thermal cycler using Q5 high-fidelity 2× master mix (New England Biolabs, M0492S). The TIDE oligonucleotide sequences used were as follows: Fwd-FSP1-Cterm, 5′-CCGAGGCGGGCAGATCACCT-3′;

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8/6/2019PCR Master Mix for Superior Performance for a Broad Range of Amplicons (NEB) Atul SinghIt works well for all kinds of PCR.

Q5 High-Fidelity 2X Master Mix

Q5 High-Fidelity 2X Master Mix from New England Biolabs

New England Biolabs

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Q5 High-Fidelity 2X Master Mix from New England Biolabs

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