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Q5 High-Fidelity 2X Master Mix
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Q5 High-Fidelity 2X Master Mix
Q5 High-Fidelity 2X Master Mix from New England Biolabs
(1)
Write a review
Citations:
(304)
Supplier Page
Supplier Page from
New England Biolabs for
Q5 High-Fidelity 2X Master Mix
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Q5 High-Fidelity 2X Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0492S
Quantity
100 reactions
Applications
DNA Analysis
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (304)
(61)
Engineering Functional Membrane–Membrane Interfaces by InterSpy
Small
March 1, 2023
Hossein Moghimianavval, Chintan Patel, Sonisilpa Mohapatra, Sung‐Won Hwang, Tunc Kayikcioglu, Yashar Bashirzadeh, Allen P. Liu, Taekjip Ha
amplified from the purchased plasmids using either Phusion (
NEB
,
M0531S
) or Q5 High Fidelity (
NEB
,
M0492S
) master mixes. The primers used are specified in Table S3. The gene fragments were assembled using Gibson
(62)
In vitro assembly of plasmid DNA for direct cloning in Lactiplantibacillus plantarum WCSF1
PLOS ONE
February 16, 2023
Marc Blanch-Asensio, Sourik Dey, Shrikrishnan Sankaran
h. Molecular biology
Q5 High Fidelity 2X Master Mix
(
New England Biolabs
GmbH [
NEB
], Germany, No.
M0492S
) was used to perform DNA
amplification
. Amplified DNA products were purified using the
Wizard® SV Gel and PCR Clean-Up System
(63)
Loss of calpain3b in Zebrafish, a Model of Limb-Girdle Muscular Dystrophy, Increases Susceptibility to Muscle Defects Due to Elevated Muscle Activity
Genes (Basel)
February 15, 2023
Sergey V. Prykhozhij, Lucia Caceres, Kevin Ban, Anna Cordeiro-Santanach, Kanneboyina Nagaraju, Eric P. Hoffman, Jason N. Berman
mutation consequences on the corresponding transcripts, we used
Q5 High-Fidelity 2X Master Mix
(
NEB
,
M0492S
) to amplify several regions in capn3b transcripts with capn3b_cDNA-del_assay, capn3b_fullCDNA, and capn3b_RLtest
(64)
Localization of Multiple Jellyfish Toxins Shows Specificity for Functionally Distinct Polyps and Nematocyst Types in a Colonial Hydrozoan
Toxins (Basel)
February 13, 2023
Anna M. L. Klompen, Matthew K. Travert, Paulyn Cartwright
450030
), and then
PCR
amplified using standard M13 primers and
Q5 High-Fidelity 2X Master Mix
(
NEB
, Cat.
M0492
). The following products were
PCR
purified using a QIAquick PCR Purification Kit (
Qiagen
, Cat.
28104
)
(65)
Whole-genome comparisons identify repeated regulatory changes underlying convergent appendage evolution in diverse fish lineages
bioRxiv
February 4, 2023
Heidi I. Chen, Yatish Turakhia, Gill Bejerano, David M. Kingsley
Ltd) and generated
PCR
amplicons using
Q5 High-Fidelity 2X Master Mix
(
New England Biolabs
catalog #
M0492
),
Phusion High-Fidelity PCR Master Mix with HF Buffer
(
New England Biolabs
catalog # M0531), and/or
(66)
Single-cell sortChIC identifies hierarchical chromatin dynamics during hematopoiesis
Nature Genetics
February 1, 2023
Peter Zeller, Jake Yeung, Helena Viñas Gaza, Buys Anton de Barbanson, Vivek Bhardwaj, Maria Florescu, Reinier van der Linden, Alexander van Oudenaarden
small RNA barcodes and handles is performed by adding 25 μl of
NEBNext Ultra II Q5 Master Mix
(
NEB
,
M0492L
), 11 μl nuclease-free water and 2 μl of RP1 and RPIx primers (10 μM). In step 40,
PCR
is performed with
(67)
RNA mis-splicing drives viral mimicry response after DNMTi therapy in SETD2-mutant kidney cancer
Cell Reports
January 31, 2023
Hong-Tao Li, H. Josh Jang, Krizia Rohena-Rivera, Minmin Liu, Hemant Gujar, Justin Kulchycki, Shuqing Zhao, Sandrin Billet, Xinyi Zhou, Daniel J. Weisenberger,
et al.
clear cDNA Synthesis Kit,Bio-Rad,Cat#172–5035
Q5 High-Fidelity 2X Master Mix
,
New England Biolabs
,Cat#
M0492L
Direct-zol RNA MiniPrep
Kit,
Zymo Research
,Cat#
R2070
RNeasy Mini Kit,
Qiagen
,Cat#
74106
CellTiter-Glo®
(68)
Expression of glycerol-3-phosphate acyltransferase increases non-polar lipid accumulation in Nannochloropsis oceanica
Microbial Cell Factories
January 16, 2023
Christian Südfeld, Aamna Kiyani, Katrin Wefelmeier, René H. Wijffels, Maria J. Barbosa, Sarah D’Adamo
primers oCSG13 and oCSG14. All PCRs for
cloning
purposes were carried out using Q5 DNA polymerase (
NEB
#
M0492
).
Genotyping
PCRs were carried out using Phire DNA polymerase (
Thermo Fisher Scientific
#
F160
). Transformation
(69)
Systemic administration of novel engineered AAV capsids facilitates enhanced transgene expression in the macaque CNS
Med
January 13, 2023
Stanton AC, Lagerborg KA, Tellez L, Krunnfusz A, King EM, Ye S, Solomon IH, Tabebordbar M, Sabeti PC.
#
S1419S
TURBO DNase,Thermo Fisher,Cat #AM2239
Q5 High-Fidelity 2X Master Mix
,
New England Biolabs
,Cat #
M0492
HRP/DAB micropolymer
IHC
detection kit,
Abcam
,Cat #
ab236466
Mouse
on
Mouse
(M.O.M.) immunodetection
(70)
Aclarubicin stimulates RNA polymerase II elongation at closely spaced divergent promoters
bioRxiv
January 13, 2023
Matthew Wooten, Brittany Takushi, Kami Ahmad, Steven Henikoff
universal i5 and a uniquely barcoded i7 primer and amplified with
NEB
Q5 high-fidelity 2× master mix (cat#
M0492S
). The libraries were purified with 1.1× volume of Sera-Mag carboxylate-modified magnetic beads and subjected
(71)
Whole-genome functional characterization of RE1 silencers using a modified massively parallel reporter assay
Cell Genomics
January 11, 2023
Kousuke Mouri, Hannah B. Dewey, Rodrigo Castro, Daniel Berenzy, Susan Kales, Ryan Tewhey
pMPRAduo:minP:GFP (addgene pending) in 1600 μL volume containing 800 μL of
Q5 High-Fidelity 2X Master Mix
(
NEB
,
M0492L
) and 0.5 μM forward and reverse primers (primers 7 and 8 for vector A and primers 9 and 10 for vector
(72)
Lipid-Peptide-mRNA Nanoparticles Augment Radioiodine Uptake in Anaplastic Thyroid Cancer
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
January 1, 2023
Li Q, Zhang L, Lang J, Tan Z, Feng Q, Zhu F, Liu G, Ying Z, Yu X, Feng H,
et al
.
The template for IVT was the
PCR product
from plasmid pST1374 using
Q5 High Fidelity 2X Master Mix
(
M0492
,
NEB
, USA). The template contains the T7 promoter and the target sequence is provided in Table S1 (Supporting
(73)
Targeted degradation via direct 26S proteasome recruitment
Nature Chemical Biology
January 1, 2023
Charlene Bashore, Sumit Prakash, Matthew C. Johnson, Ryan J. Conrad, Ivy A. Kekessie, Suzie J. Scales, Noriko Ishisoko, Tracy Kleinheinz, Peter S. Liu, Nataliya Popovych,
et al.
oligonucleotides are provided in the Supplementary Information) using the
NEB
Q5 high-fidelity polymerase (
M0492
). Two
clone
profiles were further analyzed: (1)
clones
that showed a complete loss of the wild-type
(74)
Nanobody derived using a peptide epitope from the spike protein receptor-binding motif inhibits entry of SARS-CoV-2 variants
Journal of Biological Chemistry
January 1, 2023
Nivya Mendon, Rayees A. Ganie, Shubham Kesarwani, Drisya Dileep, Sarika Sasi, Prakash Lama, Anchal Chandra, Minhajuddin Sirajuddin
(5′-ctcctgaccctcctcccccgtaaaaggaggtctgaacatc-3′) primers using Q5 polymerase
PCR
(
NEB
; catalog no.:
M0492L
). This amplified construct of ACE2 was cloned into a pTRIP
chicken
β-actin (CAG) vector (46). This construct
(75)
Human JAK1 gain of function causes dysregulated myelopoeisis and severe allergic inflammation
JCI insight
December 22, 2022
Biggs CM, Cordeiro-Santanach A, Prykhozhij SV, Deveau AP, Lin Y, Del Bel KL, Orben F, Ragotte RJ, Saferali A, Mostafavi S,
et al
.
creation. We amplified JAK1WT and JAK1A634D cDNA
PCR
products from pCMV6-Entry vectors using Q5 (
NEB
,
M0492S
) with
PacI
-JAK1_for (ggacttaattaagccaccatggctttctgtgctaaaatg) and
AscI
-JAK1_rev (gatcggcgcgccaaccttatcgtcgtcatcct)
(76)
Efficient modification and preparation of circular DNA for expression in cell culture
Communications Biology
December 21, 2022
Roman Teo Oliynyk, George M. Church
Hot Start - Readymix,KAPA Biosystems,Cat#KK2602 Q5 High-Fidelity Master Mix,
New England BioLabs
,Cat#
M0492L
NEBNext Ultra II Q5 Master Mix
,
New England BioLabs
,Cat#
M0544L
QIAquick PCR Purification Kit,
QIAGEN
,Cat#
28106
"
Sodium Acetate Solution (3 M), pH 5.2
(77)
CRISPR-Cas9-induced gene knockout in zebrafish
STAR Protocols
December 16, 2022
Raghavender Medishetti, Keerthana Balamurugan, Krishnaveni Yadavalli, Rita Rani, Aarti Sevilimedu, Anil Kumar Challa, Kishore Parsa, Kiranam Chatti
P9541 Proteinase K,Takara,Catalog # 740506 Q5® High-Fidelity 2× Master Mix,
New England Biolabs
,Catalog #
M0492L
RNase A
,
Thermo Scientific
,Catalog #
AM2270
RNase-Free Water,Takara,Catalog # 9012 Sea Salt,Instant
(78)
Macrophage NFATC2 mediates angiogenic signaling during mycobacterial infection
Cell reports
December 13, 2022
Brewer WJ, Xet-Mull AM, Yu A, Sweeney MI, Walton EM, Tobin DM.
M0323L
rSAP,
NEB
,Cat#
M0371L
Q5 High-Fidelity DNA Polymerase
,
NEB
,Cat#
M0491L
Q5 High-Fidelity 2X Master Mix
,
NEB
,Cat#
M0492L
Deoxynucleotide (dNTPs) Solution Mix,
NEB
,Cat#
N0447L
XbaI
,
NEB
,Cat#
R0145L
DpnI
,
NEB
,Cat#
R0176L
XmaI
,
NEB
,Cat#
(79)
An in silico method to assess antibody fragment polyreactivity
Nature Communications
December 7, 2022
Edward P. Harvey, Jung-Eun Shin, Meredith A. Skiba, Genevieve R. Nemeth, Joseph D. Hurley, Alon Wellner, Ada Y. Shaw, Victor G. Miranda, Joseph K. Min, Chang C. Liu,
et al.
the FACS step of each AHEAD cycle. PCRs were performed with Q5 Master Mix (
New England Biolabs
Cat#
M0492S
) and primers NGS_p1_F and NGS_p1_R. Following
PCR reactions
, samples were
PCR
purified.
Amplicon sequencing
(80)
Characterization of an RNA binding protein interactome reveals a context-specific post-transcriptional landscape of MYC-amplified medulloblastoma
Nature Communications
December 6, 2022
Michelle M. Kameda-Smith, Helen Zhu, En-Ching Luo, Yujin Suk, Agata Xella, Brian Yee, Chirayu Chokshi, Sansi Xing, Frederick Tan, Raymond G. Fox,
et al.
cDNA was quantified using
qPCR
with subsequent
PCR amplification
using 2× Q5 PCR master mix (
NEB
, #
M0492L
). The DNA was then electrophoresed in a 3% low melting agarose gel and the library extracted using Qiagen
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Reviews
Write a Review
(1)
8/6/2019
PCR Master Mix for Superior Performance for a Broad Range of Amplicons (NEB)
Atul Singh
It works well for all kinds of PCR.
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Q5 High-Fidelity 2X Master Mix from New England Biolabs
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