ProtoScript II Reverse Transcriptase M0368S from New England Biolabs

Product Specs
ItemProtoScript II Reverse Transcriptase
CompanyNew England Biolabs
Price $99.00 Inquire
Catalog NumberM0368S
Size4000 units
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Poldip2 deficiency attenuates lung disease severity in a mouse model of COVID-19PLoS OneApril 29, 2026Ruinan Hu, Alejandra Valdivia, Taylor White, Willy Ju, Maegan L. Brockman, Zhan Zhang, Hongyan Qu, Georgette Gafford, Giji Joseph, Samantha Burton, et al.(R2080, Zymo Research). Reverse transcription was performed using Protoscript II reverse transcriptase (M0368, New England Biolabs) with random primers. cDNA was amplified with 2X Forget-Me-Not EvaGreen qPCR Master Mix

(2) Host-transposon mutualism supports regeneration in planariansDevelopmental CellMarch 30, 2026Hae-Lim Lee, Axel Poulet, Sudheesh Allikka Allikka Parambil, Josien C. van WolfswinkelBio,Cat#23966-100 Proteinase K,Invitrogen,Cat#25530049 Protoscript II RT,New England Biolabs,Cat#M0368L RNaseH,New England Biolabs,Cat#M0297L Scal-HF,New England Biolabs,Cat#R3122S Terminal Transferase,New England Biolabs

(3) Self-clustering of three CBX2 molecules drives PRC2 to promote facultative heterochromatinization of Polycomb target genesMol CellMarch 19, 2026Steven Ingersoll, Abby Trouth, J. Carlos Angel, Xinlong Luo, Axel Espinoza, Joey Wen, Chengjie Zhu, Joseph Tucker, Kalkidan Astatike, Christopher J. Phiel, et al.DG-NUC-001 HaloTag® Mammalian Pull-Down,Promega,Cat# G6501 ProtoScriptII cDNA Synthesis Kit,NEB,Cat# M0368S StepOne™ Real-Time PCR System,Applied Biosystems,N/A NEBNext® Ultra™ II Directional RNA Library Prep

(4) Regulation of YAP activity by nuclear G-actin bindingNucleic Acids ResMarch 19, 2026Hong Wang, Irma M Jayawardana, Justus M Fleisch, Dennis Frank, Bing Zhao, Christos Kamaras, Peter Gebhardt, Julian Knerr, Svenja Ulferts, Shamphavi Sivabalasarma, et al.(New England Biolabs, M0307S) and 1 µL ProtoScript® II Reverse Transcriptase (New England Biolabs, M0368L). Quantitative PCR (qPCR) for gene expression and CHIP-qPCR analysis was performed in a 20-µl reaction

(5) Mycobacterium tuberculosis MutT4/RppH is an RNA pyrophosphohydrolase that forms condensate-like bodies and impacts mRNA degradationNucleic Acids ResMarch 14, 2026J Hilario Cafiero, Junpei Xiao, Irene Lepori, Abigail R Rapiejko, Manchi Reddy, Opeyemi I Ibitoye, Louis A Roberts, James C Sacchettini, M Sloan Siegrist, Scarlet S ShellThen, the sample was mixed with 100 U of ProtoScript II Reverse Transcriptase (New England Biolabs, M0368), 10 U of RNase Inhibitor Murine, 0.5 mM dNTPs, 5 mM DTT, and 1× ProtoScript II Reverse Transcriptase

(6) Characterization of gRNA-dependent and gRNA-independent off-target binding sites of PspCas13b and RfxCas13d in mammalian cellsNucleic Acids ResearchFebruary 23, 2026Huanhuan Feng, Zhixue Li, Hongdao Zhang, Yuli Zheng, Beiying Xu, Yao Zhang, Lin Zou, Ligang WuBriefly, 10 ng of total RNA was reverse transcribed using ProtoScript II Reverse Transcriptase (NEB, M0368). After PCR pre-amplification, cDNA libraries were purified using Agencourt Ampure XP beads (Beckman Coulter

(7) Genome-wide profiling of RNA 2’-O-methylation in neurons and identification of orphan snoRNA targetsResearch SquareJanuary 20, 2026Gordon Carmichael, Xuan Ye, Yaling Liu, Yinzhou Zhu, Saran Alshawi, Brittany Elliott, Christopher Holley, Jean-Denis Beaudoin, Brenton R. GraveleyEngland Biolabs, #B1034A), and 2 μL of ProtoScript II Reverse Transcriptase (New England Biolabs, #M0368S) were added to bring the final reaction volume to 40 μL. The reaction was incubated at 50°C for 1 hour

(8) Massively Scalable Single-cell Multiomic Profiling of T Cell Repertoire with REFLEXResearch SquareDecember 23, 2025Matthew R. Skene, Zachary J. Thomson, Saransh N. Thomson, Saransh Ilcisin, Matthieu Landreau, Tyanna J. Landreau, Peter J. Stuckey, Micheal Wittig, Chase D. Keller, Troy R. Torgerson, et al.dithiothreitol, 3.3 μL Superase-IN (see above), and 10 μL ProtoScript II RT enzyme (New England Biolabs, M0368L). 60 μL of RT master mix per sample/well was added to pre-existing 40 μL of volume for a final volume

(9) Regulation of a nickel tolerance operon conserved in Mesorhizobium strains from serpentine soilsApplied and Environmental MicrobiologyDecember 23, 2025Kyson T. Jensen, Joel S. GriffittsProtoScript II Buffer, 0.5 mM dNTPs, 10 µM DTT, and 200 U of ProtoScript II Reverse Transcriptase (NEB M0368). The mixture was incubated at 42°C for 1 h. After incubation, the product was purified using a Zymo

(10) Collision-induced ribosome degradation driven by ribosome competition and translational perturbationsNat CommunDecember 12, 2025Sihan Li, Okuto Shounai, Misaki Kato, Ken Ikeuchi, Toshifumi Inadafragments were then subjected to reverse transcription using ProtoScript II Reverse Transcriptase (# M0368, NEB), circularization with CircLigase™ II ssDNA Ligase (# CL9025K, Epicenter), and subsequent PCR amplification

(11) REFLEX, a Novel Immune Profiling Assay, Combining TCR Repertoire and Multiome at Massively Scalable Single-cell Resolution to Catapult Exploration of T-cell Derived ImmunitybioRxivDecember 2, 2025Matthew R. Hart, Zachary J. Thomson, Saransh N. Kaul, Saskia Ilcisin, Matthieu Landreau, Tyanna J. Stuckey, Peter J. Wittig, Micheal Keller, Chase D. McCann, Troy R. Torgerson, et al.dithiothreitol, 3.3 μL Superase-IN (see above), and 10 μL ProtoScript II RT enzyme (New England Biolabs, M0368L). 60 μL of RT master mix per sample/well was added to pre-existing 40 μL of volume for a final volume

(12) Decapping activators Edc3 and Scd6 act redundantly with Dhh1 in post-transcriptional repression of starvation-induced pathwayseLifeNovember 25, 2025Rakesh Kumar, Fan Zhang, Shreyas Niphadkar, Chisom Onu, Anil Kumar Vijjamarri, Miriam L Greenberg, Sunil Laxman, Alan G Hinnebuschcolumn,Zymo Research,Cat # D4060, Commercial assay or kit,Protoscript II,New England Biolabs,Cat # M0368L, Commercial assay or kit,CircLigase ssDNA Ligase,Epicenter,Cat # CL4111K, Commercial assay or kit,Phusion

(13) TGFβ-Smad3 signaling restores cell-autonomous Srsf1-mediated splicing of fibronectin in aged skeletal muscle stem cellsNature CommunicationsNovember 21, 2025Yuguo Liu, Svenja C. Schüler, Simon Dumontier, Frederic Balg, Sonia Bedard, Thibaut Desgeorges, Jerome N. Feige, Pierre-Luc Boudreault, C. Florian Bentzingerthe manufacturer’s instructions. cDNA was generated using ProtoScript II Reverse Transcriptase (NEB, M0368) with 15-mers oligo-dT (Integrated DNA Technologies). For densitometric quantification of cDNA, PCR amplification

(14) The CCR4-NOT deadenylase complex mediates tubulin autoregulation via specific adapters CNOT10 and CNOT11MicroPubl BiolNovember 18, 2025Stephanie L. Sarbanes, J. Robert Hogg, Antonina Roll-Mecakconverted to cDNA using an oligod(T)18 primer (NEB S1316) and Protoscript II reverse transcriptase (NEB M0368) and quantified by RT-qPCR as above, with 5’-biased gene-specific primers including those for GAPDH

(15) Loss of histone macroH2A1.1 causes kidney abnormalities secondary to a change in nutrient metabolizationSci AdvOctober 24, 2025René Winkler, Gemma Comas-Armangué, David Corujo, Adrián Sanz-Moreno, Julia Calzada-Wack, Shubhra Ashish Bhattacharya, Birgit Rathkolb, Nathalia Romanelli Vicente Dragano, Colina X. Qiao, Valentina Chiodi, et al.manufacturer’s instructions. cDNA was obtained using the ProtoScript II Reverse Transcriptase (catalog # M0368, New England Biolabs Inc., Ipswich, MA, USA). A 1:5 dilution of the cDNA was used for PCR with the Macroh2a2_exon_2

(16) The circadian regulator PER1 inhibits osteoclastogenesis by activating inflammatory genesbioRxivSeptember 24, 2025Nobuko Katoku-Kikyo, Elizabeth K. Vu, Samuel Mitchell, Ismael Y. Karkache, Elizabeth W. Bradley, Nobuaki KikyoDS-11 FX+ (Denovix). cDNA was synthesized with ProtoScript II Reverse Transcriptase (New England Biolabs, M0368L). qPCR was performed with the primers listed in Supplementary Table 2 and qPCRBIO SyGreen Blue Mix Lo-ROX

(17) Mapping the genetic landscape of iron metabolism uncovers the SETD2 methyltransferase as a modulator of iron fluxScience AdvancesSeptember 19, 2025Anthony W. Martinelli, Chun-Pei Wu, Tristan Vornbäumen, Hudson W. Coates, Louise H. Jordon, Niek Wit, Jia J. Sia, Anneliese O. Speak, James A. Nathantranscribed using ProtoScript II Reverse Transcriptase (NEB) according to their standard protocol (NEB, #M0368). Template cDNA (40 ng) was amplified using SYBR Green PCR Master Mix (Thermo Fisher Scientific, 4309155)

(18) BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4+ T cellsCell Reports MedicineSeptember 16, 2025Hongbo Gao, Yuhao Li, Ritudwhaj Tiwari, Marilia Pinzone, Xiwen Qin, Kolin M. Clark, Sara K. Nicholson, Tony Yao, Kelly Rome, Michael Scaglione, et al.protein,IDT,1081059 Random Primers,Invitrogen,48190–011 ProtoScript II Reverse Transcriptase,NEB,M0368 "RNase Inhibitor, Murine",NEB,M0314L Oligo(dT)12–18 Primer,Invitrogen,18418012 TaqMan™ Fast Advanced Master Mix for qPCR

(19) APOBEC3B Promotes SARS-CoV-2 Through Activation of PKR/eIF2⍺ and AMPD2 DysregulationVirusesAugust 28, 2025Benjamin Fixman, Lavanya Manjunath, Philip Sell, Shanshan Wang, Tamara Margaryan, Connor Qiu, Hanjing Yang, Rémi Buisson, Xiaojiang S. Chentotal RNA was reverse-transcribed into complementary cDNA using Protoscript II (New England Biolabs, #M0368S (Ipswich, MA, USA)) in a 20 µL reaction containing 1 µL of 100µM gene-specific reverse primer or 2 µL

(20) Restoration of Osimertinib sensitivity in lung cancer through BRD4 inhibitor-mediated depalmitoylation of mutant EGFR via APT1NPJ Precis OncolAugust 28, 2025Wolong Zhou, Shaoqiang Wang, Zhenyu Zhang, Linfeng Li, Jiebo Zhu, Hang Lin, Heng Zhangmin) generated 200–300 bp fragments for cDNA synthesis using ProtoScript II Reverse Transcriptase (#M0368, New England Biolabs) and dUTP-based strand marking. Adapter-ligated libraries were size-selected (200–500

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ProtoScript II Reverse Transcriptase

ProtoScript II Reverse Transcriptase from New England Biolabs

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ProtoScript II Reverse Transcriptase from New England Biolabs

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