ProtoScript II Reverse Transcriptase M0368S from New England Biolabs

Product Specs
ItemProtoScript II Reverse Transcriptase
CompanyNew England Biolabs
Price $99.00 Inquire
Catalog NumberM0368S
Size4000 units
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A RUNX2 GFP reporter is expressed prior to osteochondral differentiation and models Metaphyseal Dysplasia with Maxillary Hypoplasia and Brachydactyly (MDMHB)Journal of Bone and Mineral ResearchMay 29, 2026Dimitrios V Bikas, Sara Vardabasso, Gabrielle Quickstad, Andrew N Wyper, Karl B Shpargelsorted cells was isolated (Trizol: Thermo-Fisher 15596026) and cDNA synthesized with ProtoScript II (NEB M0368L) prior to real time qPCR (Biorad 1725201) as described35 for Runx2, Sp7, or Gapdh. Primers available

(2) SnoRNA Expression and RNA 2’-O-Methylation in Drosophila melanogaster S2 CellsbioRxivMay 26, 2026Xuan Ye, Yaling Liu, Sara Olson, Lijun Zhan, Gordon G. Carmichael, Brenton R. Graveleyhour with the ligated RNA product, RT primer and ProtoScript II RT polymerase (New England Biolabs, #M0368S). Subsequently, the RNA was degraded with 1 M Sodium Hydroxide (Fisher Chemical, #SS266-1) at 95°C for

(3) Near-Source Wastewater Surveillance of SARS-CoV-2, Influenza A, and Respiratory Syncytial Virus With Spatiotemporal Evaluation of Pepper Mild Mottle Virus as a Fecal IndicatorWater Environ ResMay 15, 2026Yuzhu Mao, Chunfu Liu, Rushan Abayagunawardena, Kosala Desilva, Patricia Arcellana, Birthe V. Kjellerupconcentrate with 0.6 mL of lysis buffer, yielding a 0.1‐mL elution. cDNA synthesis followed the NEB #M0368 protocol (New England Biolabs, MA, USA) with dNTP and random hexamer volumes specified in Table S1. 2.4

(4) Poldip2 deficiency attenuates lung disease severity in a mouse model of COVID-19PLoS OneApril 29, 2026Ruinan Hu, Alejandra Valdivia, Taylor White, Willy Ju, Maegan L. Brockman, Zhan Zhang, Hongyan Qu, Georgette Gafford, Giji Joseph, Samantha Burton, et al.(R2080, Zymo Research). Reverse transcription was performed using Protoscript II reverse transcriptase (M0368, New England Biolabs) with random primers. cDNA was amplified with 2X Forget-Me-Not EvaGreen qPCR Master Mix

(5) Polyphosphate modulates the stress-responsive formation of functional RNA-protein condensates in bacteria and mammalian cellsPLOS BiologyApril 27, 2026Jian Guan, Rebecca Lee Hurto, Akash Rai, Janakraj Bhattrai, Christopher A. Azaldegui, Luis A. Ortiz-Rodríguez, Quancheng Liu, Julie S. Biteen, Lydia Freddolino, Ursula Jakobtranscription. The RNA-DNA was reverse transcribed using ProtoScript II Reverse Transcriptase (200 units of M0368S, NEB) which was mixed into each reaction after the addition of DTT (10 mM final) and murine RNase Inhibitor

(6) Proximity proteomics reveals a role for IFI16 during human coronavirus infectionbioRxivApril 27, 2026Sylvester Languon, Ian Bailey, Madeleine Sorensen, Zachary D. Miller, Jihui Sha, Jake Dearborn, William Dowell, Tylar Kirch, James Wohlschlegel, Devdoot MajumdarScientific). A total of 300 ng of RNA was used to generate cDNA using protoscript II reverse transcriptase (M0368, New England biolabs Inc) and diluted 1:20 in DNAse free water before being used as template for qPCR

(7) Chaperone saturation mediates translation and protein folding efficiencybioRxivApril 12, 2026Andrew T. Martens, Vincent J. Hilseret al., 2012), with modifications: ProtoScript II reverse transcriptase (New England Biolabs cat no. M0368) was used for reverse transcription, and different biotinylated oligonucleotides were used for the removal

(8) HP-Bodies – Ancestral Condensates that Regulate RNA Turnover and Protein Translation in BacteriabioRxivApril 11, 2026Jian Guan, Rebecca Lee Hurto, Akash Rai, Christopher A. Azaldegui, Luis A. Ortiz-Rodríguez, Julie S. Biteen, Lydia Freddolino, Ursula Jakobtranscription. The RNA-DNA was reverse transcribed using ProtoScript II Reverse Transcriptase (200 units of M0368S, NEB) which was mixed into each reaction after the addition of DTT (10mM final) and murine RNase Inhibitor

(9) Host-transposon mutualism supports regeneration in planariansDevelopmental CellMarch 30, 2026Hae-Lim Lee, Axel Poulet, Sudheesh Allikka Allikka Parambil, Josien C. van WolfswinkelBio,Cat#23966-100 Proteinase K,Invitrogen,Cat#25530049 Protoscript II RT,New England Biolabs,Cat#M0368L RNaseH,New England Biolabs,Cat#M0297L Scal-HF,New England Biolabs,Cat#R3122S Terminal Transferase,New England Biolabs

(10) Self-clustering of three CBX2 molecules drives PRC2 to promote facultative heterochromatinization of Polycomb target genesMol CellMarch 19, 2026Steven Ingersoll, Abby Trouth, J. Carlos Angel, Xinlong Luo, Axel Espinoza, Joey Wen, Chengjie Zhu, Joseph Tucker, Kalkidan Astatike, Christopher J. Phiel, et al.DG-NUC-001 HaloTag® Mammalian Pull-Down,Promega,Cat# G6501 ProtoScriptII cDNA Synthesis Kit,NEB,Cat# M0368S StepOne™ Real-Time PCR System,Applied Biosystems,N/A NEBNext® Ultra™ II Directional RNA Library Prep

(11) Regulation of YAP activity by nuclear G-actin bindingNucleic Acids ResMarch 19, 2026Hong Wang, Irma M Jayawardana, Justus M Fleisch, Dennis Frank, Bing Zhao, Christos Kamaras, Peter Gebhardt, Julian Knerr, Svenja Ulferts, Shamphavi Sivabalasarma, et al.(New England Biolabs, M0307S) and 1 µL ProtoScript® II Reverse Transcriptase (New England Biolabs, M0368L). Quantitative PCR (qPCR) for gene expression and CHIP-qPCR analysis was performed in a 20-µl reaction

(12) Mycobacterium tuberculosis MutT4/RppH is an RNA pyrophosphohydrolase that forms condensate-like bodies and impacts mRNA degradationNucleic Acids ResMarch 14, 2026J Hilario Cafiero, Junpei Xiao, Irene Lepori, Abigail R Rapiejko, Manchi Reddy, Opeyemi I Ibitoye, Louis A Roberts, James C Sacchettini, M Sloan Siegrist, Scarlet S ShellThen, the sample was mixed with 100 U of ProtoScript II Reverse Transcriptase (New England Biolabs, M0368), 10 U of RNase Inhibitor Murine, 0.5 mM dNTPs, 5 mM DTT, and 1× ProtoScript II Reverse Transcriptase

(13) Characterization of gRNA-dependent and gRNA-independent off-target binding sites of PspCas13b and RfxCas13d in mammalian cellsNucleic Acids ResearchFebruary 23, 2026Huanhuan Feng, Zhixue Li, Hongdao Zhang, Yuli Zheng, Beiying Xu, Yao Zhang, Lin Zou, Ligang WuBriefly, 10 ng of total RNA was reverse transcribed using ProtoScript II Reverse Transcriptase (NEB, M0368). After PCR pre-amplification, cDNA libraries were purified using Agencourt Ampure XP beads (Beckman Coulter

(14) Direct delivery of assay reagents to extracellular vesicles in liquid biopsies for biomarker analysisNature ProtocolsJanuary 30, 2026Bo Ning, Long Chen, Brady M. Youngquist, Christopher J. Lyon, Yun Su, Tony Hu(New England Biolabs, cat. no. M0653T)ProtoScript II Reverse Transcriptase (New England Biolabs, cat no. M0368L)NEBuffer 2.1 (New England Biolabs, cat. no. B7202 (previously B6002S))Zymo Quick-DNA/RNA Viral Kit (Zymo Research

(15) Genome-wide profiling of RNA 2’-O-methylation in neurons and identification of orphan snoRNA targetsResearch SquareJanuary 20, 2026Gordon Carmichael, Xuan Ye, Yaling Liu, Yinzhou Zhu, Saran Alshawi, Brittany Elliott, Christopher Holley, Jean-Denis Beaudoin, Brenton R. GraveleyEngland Biolabs, #B1034A), and 2 μL of ProtoScript II Reverse Transcriptase (New England Biolabs, #M0368S) were added to bring the final reaction volume to 40 μL. The reaction was incubated at 50°C for 1 hour

(16) Genome-wide profiling of RNA 2’- O -methylation in neurons and identification of orphan snoRNA targetsbioRxivJanuary 1, 2026Xuan Ye, Yaling Liu, Yinzhou Zhu, Sarah A. Alshawi, Brittany A. Elliott, Christopher L. Holley, Jean-Denis Beaudoin, Brenton R. Graveley, Gordon G. CarmichaelEngland Biolabs, #B1034A), and 2 μL of ProtoScript II Reverse Transcriptase (New England Biolabs, #M0368S) were added to bring the final reaction volume to 40 μL. The reaction was incubated at 50°C for 1 hour

(17) Regulation of a nickel tolerance operon conserved in Mesorhizobium strains from serpentine soilsApplied and Environmental MicrobiologyDecember 23, 2025Kyson T. Jensen, Joel S. GriffittsProtoScript II Buffer, 0.5 mM dNTPs, 10 µM DTT, and 200 U of ProtoScript II Reverse Transcriptase (NEB M0368). The mixture was incubated at 42°C for 1 h. After incubation, the product was purified using a Zymo

(18) Massively Scalable Single-cell Multiomic Profiling of T Cell Repertoire with REFLEXResearch SquareDecember 23, 2025Matthew R. Skene, Zachary J. Thomson, Saransh N. Thomson, Saransh Ilcisin, Matthieu Landreau, Tyanna J. Landreau, Peter J. Stuckey, Micheal Wittig, Chase D. Keller, Troy R. Torgerson, et al.dithiothreitol, 3.3 μL Superase-IN (see above), and 10 μL ProtoScript II RT enzyme (New England Biolabs, M0368L). 60 μL of RT master mix per sample/well was added to pre-existing 40 μL of volume for a final volume

(19) Collision-induced ribosome degradation driven by ribosome competition and translational perturbationsNat CommunDecember 12, 2025Sihan Li, Okuto Shounai, Misaki Kato, Ken Ikeuchi, Toshifumi Inadafragments were then subjected to reverse transcription using ProtoScript II Reverse Transcriptase (# M0368, NEB), circularization with CircLigase™ II ssDNA Ligase (# CL9025K, Epicenter), and subsequent PCR amplification

(20) REFLEX, a Novel Immune Profiling Assay, Combining TCR Repertoire and Multiome at Massively Scalable Single-cell Resolution to Catapult Exploration of T-cell Derived ImmunitybioRxivDecember 2, 2025Matthew R. Hart, Zachary J. Thomson, Saransh N. Kaul, Saskia Ilcisin, Matthieu Landreau, Tyanna J. Stuckey, Peter J. Wittig, Micheal Keller, Chase D. McCann, Troy R. Torgerson, et al.dithiothreitol, 3.3 μL Superase-IN (see above), and 10 μL ProtoScript II RT enzyme (New England Biolabs, M0368L). 60 μL of RT master mix per sample/well was added to pre-existing 40 μL of volume for a final volume

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ProtoScript II Reverse Transcriptase

ProtoScript II Reverse Transcriptase from New England Biolabs

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ProtoScript II Reverse Transcriptase from New England Biolabs

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