Q5 Hot Start High-Fidelity DNA Polymerase M0493L from New England Biolabs

Product Specs
ItemQ5 Hot Start High-Fidelity DNA Polymerase
CompanyNew England Biolabs
Price $596.00 Inquire
Catalog NumberM0493L
Size500 units
ApplicationsDNA Analysis
TypeDNA Polymerase
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Multi-site DMS probing reveals higher-order structure of RNA-protein complexes in living cellsMol CellApril 21, 2026Irfana Saleem, Thomas Miller, Lucas Kearns, Anthony Hoang, Joshua Meehan, Ritwika Bose, David Mitchell, David H. Price, Calla M. Olson, Chase A. Weidmann, et al.AM2238 Superscript II Reverse Transcriptase,Invitrogen,Cat# 18064014 Q5 Hot Start Polymerase,NEB,Cat# M0493L TEV Protease,Takara,Cat# 635668 Pierce Glutathione Agarose,Thermo Scientific,Cat# 16100 TALON Metal

(2) Enzymatically Controlled Release of Proteins and Peptides (ENCOREP): A Promising Alternative Secretion ApproachiScienceApril 17, 2026Daniel Ivanusic, Gregor Zipf, Josef Maier, Hubert Bernauer, Norbert Bannert,New England Biolabs,Cat# R3101 EcoRV-HF,New England Biolabs,Cat# R3195 Q5 Hotstart polymerase,New England Biolabs,Cat# M0493 DpnI,New England Biolabs,Cat# R0176S PmeI,New England Biolabs,Cat# R0560 NheI-HF,New England Biolabs,Cat#

(3) Phage-assisted evolution of allosteric protein switchesNat CommunApril 14, 2026Nicholas T. Southern, Anna von Bachmann, Alisa Hovsepyan, Marielouise Griebl, Benedict Wolf, Nina Lemmen, Ann-Sophie Kroell, Simon Westermann, Jan Mathony, Dominik Niopekfragments were amplified using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs, cat. no. M0493S). PCR products were gel-purified and assembled using NEB Type IIs restriction enzymes and T4 DNA Ligase

(4) Systematic CRISPRi screening reveals genetic modulators of E. coli isoprenoid productionbioRxivApril 10, 2026Dhiraj Dokwal, Philip M. Brown, Christine Ingle, Scott H. Saunders, Kimberly A. Reynoldssequences were appended to the sgRNA cassette using Q5 Hot Start High-Fidelity DNA Polymerase (NEB, M0493) with adapter-specific primers. For plate-level identification in the large-scale genomic screen, an

(5) Synthetic circRNAs employ IRES activity for translation in cells and in cell-free translation systemsbioRxivMarch 30, 2026Philipp Koch, Frederic S. W. Arendrup, Chaehee Lim, Samyukta Narayanan, Amina Adam, Massimiliano Clamer, Anders H. Lund, Chun-Kan Chen, Kathrin Leppekoverhangs using primers PK_352–PK_391 according to manufacturer’s recommendations (NEB Q5 Polymerase, M0493S). The plasmid backbone was digested using EcoRI-HF (NEB, R3101S) and BsiWI-HF (NEB, R3553S) restriction

(6) APC coordinates GSK3 phosphorylation of SETD8 to suppress colorectal cancerCell ReportsMarch 24, 2026Zvi Cramer, Keara Monaghan, Ricardo Petroni, Xin Wang, Stephanie Adams-Tzivelekidis, Kayla Durning, Melissa S. Kim, Yuhua Tian, Nicolette M. Johnson, María F. Carrera Rodríguez, et al.manufacturer’s instructions (Agilent #210518). Human SETD8 cDNA was cloned with Q5 polymerase (NEB #M0493S) using the following primers: SETD8-cDNA-F: 5′-CGCTCTAGAATGGCTAGAGGCAGGAAGATGTGC-3’; SETD8-cDNA-R: 5′-CGCGGATCCCCACGTGGTTAG

(7) Lysosomal down-regulation of the mu opioid receptor is opposed by the Retromer complexSci AdvMarch 20, 2026Aleksandra Dagunts, Hayden Adoff, Brandon Novy, Lamya Ben Ameur, Monica De Maria, Arpiar Saunders, Braden T. Lobingier(QIAGEN, 51104). sgRNA libraries were prepared using Q5 Hot Start High-Fidelity DNA Polymerase (NEB, M0493L) and barcoding primers. PCR products were purified using QIAquick PCR purification columns (QIAGEN,

(8) Integrated Size-Selective Cell Purification and Electroporation for Genetic Manipulation of Primary CellsMicromachines (Basel)March 15, 2026Hyun Woo Sung, Soojung Claire Hurintroduced via site-directed mutagenesis using the Q5 Hot Start High-Fidelity DNA Polymerase Kit (cat# M0493S, New England Biolabs, Ipswich, MA, USA), with the following primers: forward: 5′- ACT CAC TAT AAG GAG

(9) Identification of a cytosol-to-nucleus feedback loop that regulates neuronal microtubule nucleationJournal of Cell BiologyMarch 2, 2026Nitish Kumar, Nathaniel Carey, J. Ian Hertzler, Annabelle R. Bernard, Gibarni Mahata, Gregory O. Kothe, Yitao Shen, Melissa M. Rollsrestriction enzymes were from NEB and thermostable DNA polymerase Q5 was from NEB (Q5 hotstart NEB M0493S). The generation of pUAS-Kat60-mScarlet3 constructs The amino-terminus of Kat60 was PCR amplified with

(10) Genomic characterization of dengue virus in the Ningxia Hui Autonomous Region, China (2019 and 2023)Genome Biology and EvolutionMarch 2, 2026Longshan Li, Jun Zhan, Dongzhi Yang, Tao Li, Xiaoqiang Sun, Fang Yuan, Min Cao, Wei Zhang, Ting Mu, Jingting Wang, et al.primers (Table S2) and Q5 Hot Start High-Fidelity DNA Polymerase (New England BioLabs, catalog no. M0493L) to ensure amplification accuracy. PCR products were purified using AMPure XP beads (Beckman Coulter,

(11) In vivo CRISPR/Cas9 screens identify new regulators of B cell activation and plasma cell differentiationJournal of Experimental MedicineMarch 2, 2026Lesly Calderón, Markus Schäfer, Marina Rončević, René Rauschmeier, Markus Jaritz, Tanja A. Schwickert, Qiong Sun, Andrea Pauli, Johannes Zuber, Meinrad Busslingerusing a two-step PCR protocol. The first PCR used 0.5 μl of Q5 Hot Start High-Fidelity DNA Polymerase (M0493; New England Biolabs) in 50-μl reactions containing 100 ng of genomic DNA. For each sample, the resulting

(12) CRAGE-RB-PI-seq reveals transcriptional dynamics of plant-associated bacteria during root colonizationNature CommunicationsFebruary 21, 2026Tomoya Honda, Sora Yu, Dung Mai, Leo Baumgart, Emory M. Chan, Gyorgy Babnigg, Yasuo Yoshikuniperformed for 7 cycles in a total volume of 50 µl using Q5® Hot Start High-Fidelity DNA Polymerase (NEB: M0493L). The amplified products were subsequently gel-purified. For the conjugal vector, we used a modified

(13) Evidence that extra copies of chromosome 1q play a role in the early phases of pancreatic neoplasiaScience AdvancesFebruary 20, 2026Christopher Douville, Jeeun Parksong, Marco Dal Molin, Sarah Graham, Patricia T. Greipp, Ryan Knudson, Samuel Curtis, Yuxuan Wang, Lisa Dobbyn, Maria Popoli, et al.supplemented with an additional 0.5 U of Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs, M0493L), 1 μl of 10 mM deoxynucleoside triphosphates (Deoxynucleoside triphosphates; New England Biolabs, N0447L)

(14) Allelic Variation at 9p21.3 Orchestrates Widespread RNA Splicing Shifts Governing Vascular Smooth Muscle Cell PlasticitybioRxivFebruary 11, 2026S. Suryavanshi, H. Yang, E. Salido, V. Lo Sardo(5′ GACTTTCCCCCA CAATGAAA 3′). Genotyping PCR was performed using Q5 Hot Start DNA polymerase (NEB M0493S) in 25uL reactions. Reaction parameters include: 30 cycles, 58 °C annealing, 30sec extension, 200ng

(15) Functional correction and genome integrity with duplex base editing of β-thalassemic hematopoietic stem cellsGenome BiologyFebruary 2, 2026Nikoletta Y. Papaioannou, Petros Patsali, Julia Klermund, Panayiota L. Papasavva, Geoffroy Andrieux, Lola Koniali, Basma Naiisseh, Soteroula Christou, Maria Sitarou, Marina Kleanthous, et al.a template for a 30-cycle PCR amplification using Q5® Hot Start High-Fidelity DNA Polymerase (NEB #M0493L) and primers listed in Additional file 1: Table S8. In the case of HBG1/2, a first 20-cycle PCR specifically

(16) The evolution of genetic drift over 50,000 generationsbioRxivFebruary 1, 2026Joao A. Ascensao, QinQin Yu, Oskar Hallatschekbarcodes were amplified from genomic DNA samples using PCR with Q5 Hot Start Polymerase (NEB, cat. no. M0493S); the 50μL reactions included 5μL PCR primers, 5μL genomic DNA, 10μL 5x buffer, 10μL GC enhancer, 1μL

(17) Chemical control of 2'-hydroxyl-dependent Cas9 target engagement enables CRISPR RNA ribose replacementbioRxivJanuary 28, 2026Adrian A. Pater, Halle M. Barber, Sruthi Sudhakar, Ramadevi Chilamkurthy, Sunit K. Jana, Mansi A. Parasrampuria, Michael. S. Bosmeny, Jacob A. Graczyk-Marrs, Seth B. Eddington, Cole A. Blazier, et al.contained 30 ng of genomic DNA and were amplified with Q5 Hot Start High-Fidelity DNA Polymerase (NEB, M0493L) following the manufacturer’s protocol. The PCR program was set to 98°C for 3 min followed by 30 cycles

(18) Mechanisms of clinical resistance to selective FGFR2 inhibition by lirafugratinibAnnals of OncologyJanuary 20, 2026H. Ellis, E. R. Balasooriya, A. Varkaris, B. Hajian, J. Wan, M. Shekhar, I. Gritti, V. Vijay, L. Albertelli, S. Piot, et al.the pLV-FGFR2::PHGDH and pLVX-TetOne-Puro-FGFR2::PHGDH constructs by site-directed mutagenesis (NEB #M0493) followed by HiFi assembly. All constructs were verified by targeted Sanger sequencing. For transient

(19) Proteome-wide C-degron activity profiling connects conditional regulation of the CTLH E3 ligase complex to ribosome biogenesisbioRxivJanuary 17, 2026Drew W. Grant, Shengjiang Tan, Dylan E. Ramage, Mamie Z. Li, Ying Di, Iva A. Tchasovnikarova, Michael P. Weekes, Stephen J. Elledge, Alan J. Warren, Richard T. Timmsassociated with each ORF were amplified by 24 cycles of PCR (Q5 Hot Start High-Fidelity DNA Polymerase, NEB #M0493S) using primers (Table S6) that anneal to common regions flanking the barcode. A pool of eight “staggered”

(20) Decay of driver mutations shapes the landscape of intestinal transformationNatureJanuary 15, 2026Filipe C. Lourenço, Iannish D. Sadien, Kim Wong, Sam Adler, Ashley Sawle, Leonor Schubert Santana, Lee Hazelwood, Giada Giavara, Anna M. Nicholson, Matthew D. Eldridge, et al.Promega) and PCR was performed according to the Q5 Hot Start High-Fidelity DNA Polymerase protocol (M0493L, New England BioLabs) with enhancer, using the following primers (10 µM, 1:1:1 stoichiometry): KrasG12D,

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4/9/2015Hi Fidelity Polymerase Benjamin GerovacI used this polymerase while performing PCR to subclone a gene fragment into a viral vector so I could transduce primary epithelial cells. Upon sequencing, this polymerase gave a PCR product of the ... Read Review

Q5 Hot Start High-Fidelity DNA Polymerase

Q5 Hot Start High-Fidelity DNA Polymerase from New England Biolabs

New England Biolabs

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Q5 Hot Start High-Fidelity DNA Polymerase from New England Biolabs

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