Fig 2: Photomicrographs of cellular differentiation of the culture scaffold at day 0, 7 and 14 using H&E stain, immunohistochemical stain for both AFP and CK19 for detection of differentiated hepatocyte-like cells (arrows) showing at day 0 no detected differentiated cells, at day 7 showing increased number of differentiated cells H&E, AFP & in CK19 and at day 14 the cells increase in H&E and in CK19 while decreased in AFP immunostaining (× 400)

Fig 3: Photomicrographs from Group I (control group): a H&E-stained sections showing normal stratified epithelium of epidermal (SE) and dermal (CT) layers with normal hair follicles (K) and thin keratin layer (curved arrow). b Immunostained sections showing -ve skin reaction. Photomicrographs from Group II (subcutaneous group): c H&E-stained showing hepatocyte-like cells (arrow) at the base of a hair follicle. d Stained sections with AFP showing +ve immunostaining cells (arrow) detected at the root of hair follicles. e Stained sections with CK19 showing +ve immunostaining cells (arrow) detected at the shaft of hair follicles (× 200)