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Protein Biochemistry
Chemicals and Reagents
DAPI
DAPI from BioLegend
(2)
Write a review
Citations:
(209)
Supplier Page
Supplier Page from
BioLegend for
DAPI
Description
DAPI
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
DAPI
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
422801
Quantity
10 mg
Applications
Intracellular Staining for Flow Cytometry (ICFC), Immunocytochemistry (ICC)
Format
Lyophilized
Target
DAPI
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (209)
(1)
Mutant PIK3CA is a targetable driver alteration in histiocytic neoplasms
Blood Advances
December 12, 2023
Benjamin H. Durham, Oshrat Hershkovitz-Rokah, Omar I. Abdel-Wahab, Mariko Yabe, Young Rock Chung, Gilad Itchaki, Maayan Ben-Sasson, Vered A. Asher-Guz, David Groshar, Seyram A. Doe-Tetteh,
et al.
(
clone
M5/114.15.2,
BioLegend
,
107614
,
1:200
) following the manufacturer's protocol.
DAPI
(
BioLegend
,
422801
,
1:1000
) was used to exclude dead cells (supplemental Figure 1).
Fluorescence
-activated cell sorter
(2)
Protocol for isolation and spectral flow cytometry analysis of immune cells from the murine exocrine and endocrine pancreas
STAR Protocols
December 1, 2023
Laura Steiger, Zora Baumann, Lena Keller, Marianne Böni-Schnetzler, Marc Y. Donath, Daniel T. Meier
similar to Zombie
NIR
and can only be unmixed by spectral
flow cytometry
).Alternatives: Use
DAPI
(Cat #
422801
,
BioLegend
) for live/dead
staining
. Add
DAPI
(1.38 μg/mL (3 μM)) immediately before acquiring samples
(3)
Loss of Slc35a2 alters development of the mouse cerebral cortex
bioRxiv
December 1, 2023
Soad Elziny, Sahibjot Sran, Hyojung Yoon, Rachel R. Corrigan, John Page, Amanda Ringland, Anna Lanier, Sara Lapidus, James Foreman, Erin L. Heinzen,
et al.
were washed with 0.1M phosphate buffered saline (PBS), counterstained with
DAPI
(
1:1000
,
BioLegend
,
422801
) and mounted on glass slides using
Prolong Gold Antifade Mountant
(Invitrogen,
P36930
). Image analysis One
(4)
HPV16E1 downregulation altered the cell characteristics involved in cervical cancer development
Scientific Reports
October 25, 2023
Thanayod Sasivimolrattana, Arkom Chaiwongkot, Parvapan Bhattarakosol
(#
ab6717
;
Abcam
, USA) for another hour at 37 °C. Then, the slide was stained with 300 nM DAPI solution (#
422801
;
Biolegend
, USA) for 5 min at room temperature. Then, the slide was treated with anti-fade
fluorescence
(5)
The involvement of caspases in the process of nuclear removal during lens fiber cell differentiation
Cell Death Discovery
October 21, 2023
Rifah Gheyas, A. Sue Menko
111-545-003
) were from
Jackson ImmunoResearch Laboratories
. Nuclei were labeled with the DAPI reagent (
422801
) from
Biolegend
. Fluorescent tagging of the CAD antibody Before tagging the antibody with a fluorophore,
(6)
Effector memory T cells induce innate inflammation by triggering DNA damage and a non-canonical STING pathway in dendritic cells
Cell Reports
October 3, 2023
Hannah E. Meibers, Kathrynne A. Warrick, Andrew VonHandorf, Charles N. Vallez, Kiana Kawarizadeh, Irene Saha, Omer Donmez, Viral G. Jain, Leah C. Kottyan, Matthew T. Weirauch,
et al.
(
AZD6738
),
Selleck
Chem,
S7693
2-Mercaptoethanol
(BME),
Sigma
,M6250 "4’,6-diamidino-2-phenylindole (
DAPI
)",
Biolegend
,
422801
Paraformaldehyde aqueous solution (PFA),Fisher,15710 Fetal calf serum (FCS),
Sigma
,
F0926
RBC lysis
(7)
Generation of Novel Immunocompetent Mouse Cell Lines to Model Experimental Metastasis of High-Risk Neuroblastoma
Cancers (Basel)
September 23, 2023
Mayura R. Dhamdhere, Dan V. Spiegelman, Lisa Schneper, Amy K. Erbe, Paul M. Sondel, Vladimir S. Spiegelman
min at RT in the dark. Following a washing step (300× g for 5 min), a drop of
DAPI
(
BioLegend
, Cat #
422801
) was added to the cell suspensions, and data were acquired using a
ThermoFisher
Attune NxT
flow cytometer
.
(8)
Protocol for enrichment, purification, and cytocentrifugation of mouse liver endothelial cells
STAR Protocols
September 1, 2023
Shanin Chowdhury, Kaela Drzewiecki Fried, Yasuko Iwakiri, Joseph Brancale, Sílvia Vilarinho
Chemicals,Cat#12125-02-9 Fetal bovine serum (FBS),Gibco,Cat#10437-028
DAPI
(
1:100
),
BioLegend
,Cat#
422801
"PBS (10×), pH 7.4",
Thermo Fisher Scientific
,Cat#
70011069
"PBS (1×), pH 7.4",
Thermo Fisher Scientific
,Cat#
10010023
Percoll
,
Sigma-Aldrich
,Cat#17-0891-02 Prolong™
(9)
Epigenetic neural glioblastoma enhances synaptic integration and predicts therapeutic vulnerability
bioRxiv
August 7, 2023
Richard Drexler, Robin Khatri, Thomas Sauvigny, Malte Mohme, Cecile L. Maire, Alice Ryba, Yahya Zghaibeh, Lasse Dührsen, Amanda Salviano-Silva, Katrin Lamszus,
et al.
fixed with 4% PFA (Sigma/Aldrich), blocked with PBS containing 5% FBS, 0.1%TritonX and
DAPI
(4 ug/mL, #
422801
,
Biolegend
) for one hour at room temperature and were stained with vimentin (#
677809
,
Biolegend
), cleaved
(10)
iPSC-derived reactive astrocytes from patients with multiple sclerosis protect cocultured neurons in inflammatory conditions
Journal of Clinical Investigation
July 3, 2023
Janis Kerkering, Bakhrom Muinjonov, Kamil Sebastian Rosiewicz, Sebastian Diecke, Charlotte Biese, Juliane Schiweck, Claudia Chien, Dario Zocholl, Thomas Conrad, Friedemann Paul,
et al.
1:1,000
; all from
Invitrogen
) were given for 1 hour, and cells were counterstained with
DAPI
(
1:4,000
;
422801
,
BioLegend
); for mounting, DAKO Mounting medium was used. Images were taken on a
Leica
DMI6000 B
fluorescence microscope
(11)
Purification and functional characterization of novel human skeletal stem cell lineages
Nature Protocols
July 1, 2023
Malachia Y. Hoover, Thomas H. Ambrosi, Holly M. Steininger, Lauren S. Koepke, Yuting Wang, Liming Zhao, Matthew P. Murphy, Alina A. Alam, Elizabeth J. Arouge, M. Gohazrua K. Butler,
et al.
(Sigma-Aldrich, cat. no. C0775) 4,6-Diamidino-2-phenylindole (DAPI) (10 mg) (
BioLegend
, cat. no.
422801
) ▲CRITICAL Since
DAPI
is photosensitive, be sure to minimize exposure to light sources. Deoxyribonuclease
(12)
Immune Cells Localize to Sites of Corneal Erosions in C57BL/6 Mice
Biomolecules
June 29, 2023
Le PM, Pal-Ghosh S, Menko AS, Stepp MA.
temperature before
staining
with primary antibodies for 2.5 h at 37 °C. Nuclei were labeled with
DAPI
(#
422801
,
Biolegend
, San Diego, CA, USA) for 30min before sections were washed and mounted in
Prolong Diamond Antifade Mountant
(13)
Infection with hypervirulent Mycobacterium tuberculosis triggers emergency myelopoiesis but not trained immunity
Frontiers in Immunology
June 13, 2023
Ana Raquel Maceiras, Diogo Silvério, Rute Gonçalves, Marcos S. Cardoso, Margarida Saraiva
conjugated (Cat. #
A-31573
,
Invitrogen
) for 2 h at room temperature. Slides were stained with
DAPI
(Cat. #
422801
,
Biolegend
;
1:1000
dilution), and glass coverslips were mounted using Fluoroshield (Cat. #F6182, Sigma-Aldrich).
(14)
Elastin-targeted nanoparticles delivering doxycycline mitigate cytokine storm and reduce immune cell infiltration in LPS-mediated lung inflammation
PLOS ONE
June 2, 2023
Shivani Arora, Narendra Vyavahare
(I-A/I-E)-PE-Cy7 (cat#
25-5321-82
,
Invitrogen
), Anti mouse Siglec F-PE (Cat#
12-1702-80
,
Invitrogen
),
DAPI
(Cat#
422801
,
Biolegend
), TruStainFcX Plus (Cat#
156604
, biolegend), Anti mouse CD11b-APC (Cat#
101212
, biolegend),
(15)
Generation of endogenously tagged E-cadherin cells using gene editing via non-homologous end joining
STAR Protocols
June 1, 2023
Natalie Rimmer, Ching-Yeu Liang, Ricardo Coelho, Monica Nunez Lopez, Francis Jacob
8388.6 2-Propanol,Carl Roth,Cat# CP41.3 Hydrochloric acid 37%,Carl Roth,Cat# 9277.1
DAPI
,
BioLegend
,Cat#
422801
ProLong® Gold Antifade Reagent with DAPI
,
Cell Signaling Technology
,Cat#
8961
,, Critical commercial
(16)
Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts
Cell reports
May 30, 2023
Di Nardo A, Chang YL, Alimohammadi S, Masuda-Kuroki K, Wang Z, Sriram K, Insel PA.
Antibody,
BioLegend
,
148603
Anti-Mast Cell Chymase
antibody,
Abcam
,
Ab233103
"DAPI (4′,6-Diamidino-2-Phenylindole, Dilactate)",
BioLegend
,
422801
Recombinant
Anti-TLR2 antibody [EPR20302-119
],
Abcam
,
Ab209216
,, Bacterial and
virus
strains
,, ,,
Staphylococcus epidermidis
(17)
Initiation of fibronectin fibrillogenesis is an enzyme-dependent process
Cell Reports
May 30, 2023
Shay Melamed, Shelly Zaffryar-Eilot, Elisabeth Nadjar-Boger, Rohtem Aviram, Huaning Zhao, Wesal Yaseen-Badarne, Rotem Kalev-Altman, Dalit Sela-Donenfeld, Oded Lewinson, Sophie Astrof,
et al.
integrin (
BD biosciences
,
553715
,
1:500
). Phalloidin (
Abcam
,
ab176757
,
1:1000
) and
DAPI
(
Biolegend
,
422801
,
1:10,000
) were also used. Single
cell migration assay
FN was treated with RhLOXL3, with or without
(18)
In Vitro Effects of Methylprednisolone over Oligodendroglial Cells: Foresight to Future Cell Therapies
Cells
May 30, 2023
Ulises Gómez-Pinedo, Jordi A. Matías-Guiu, Denise Ojeda-Hernandez, Sarah de la Fuente-Martin, Ola Mohamed-Fathy Kamal, Maria Soledad Benito-Martin, Belen Selma-Calvo, Paloma Montero-Escribano, Jorge Matías-Guiu
488 or 555) for 2 h at room temperature, in darkness, counterstaining nuclei with
DAPI
(
DAPI
1:3000
,
422801
,
BioLegend
, San Diego, CA, USA) and mounted in FluorSave™ mounting medium (catalogue No. 34558920ML,
(19)
Antibody landscape of C57BL/6 mice cured of B78 melanoma via immunotherapy
bioRxiv
May 5, 2023
Anna Hoefges, Sean J McIlwain, Amy K Erbe, Nicholas N Mathers, Angie E Xu, Drew Melby, Kaitlin Tetreault, Trang Q Le, KyungMann Kim, Richard S Pinapati,
et al.
with 3ml flow buffer and resuspended in 50–100μl flow buffer. A drop of
DAPI
(
BioLegend
, catalog #
422801
) was added to each tube before data was acquired on a ThermoFisher Attune
flow cytometer
. Data analysis
(20)
Single-cell whole-genome sequencing, haplotype analysis in prenatal diagnosis of monogenic diseases
Life Science Alliance
May 1, 2023
Liang Chang, Haining Jiao, Jiucheng Chen, Guanlin Wu, Ping Liu, Rong Li, Jianying Guo, Wenqing Long, Xiaojian Tang, Bingjie Lu,
et al.
HLA-G). The enriched cells were then stained with fluorescently labeled antibodies including
DAPI
(
422801
;
BioLegend
), anti-cytokeratin FITC (
clone
C11
628608
;
BioLegend
), and anti-CD45 PE (
clone
2D1
368510
;
Page
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Reviews
Write a Review
(1)
3/28/2023
Great DAPI for Immunocytochemistry
Umut Tank
I use it for nuclear staining of HEK293 and HeLa cells. Good brightness and super easy to use it, also the amount needed is really really low. So, it will last forever.
(1)
7/12/2022
DAPI Nuclear Stain
Matthew Ratsep
I use this reagent for routine staining of nuclei in immunofluorescence. It works great in most samples and gives a bright clear signal.
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DAPI from BioLegend
Supplier Page
View Company Product Page
Pricing Info
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