Digitonin G9441 from Promega

Product Specs
ItemDigitonin
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG9441
Size40microl

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A spatial multi-omics atlas of immunosenescence reveals germinal-center B cell dysfunction in human lymph nodesCell Press BlueJuly 20, 2026Negin Farzad, Archibald Enninful, Yao Lu, Fabio Parisi, Anthony A. Fung, Yumi Kwon, Yajuan Li, Margaux Labrosse, Mingyu Yang, Francesco Strino, et al.,Thermo Fisher Scientific,AM9530G 10% (wt/vol) NP40 Substitute,Millipore-Sigma,11332473001 2% (wt/vol) Digitonin,Promega,G9441 5% BSA,Sigma-Aldrich,A8806 1 M EDTA solution pH 8.0,American Bio,AB00502 2X Tagmentation buffer,Diagenode/Illumina,C01019043/20034197 Alexa

(2) MicroRNA-29a enhances and preserves stem-like CD8 T cell differentiation by regulating master epigenetic circuits of exhaustionbioRxivJune 1, 2026Xuebing Leng, Lance A. Buchness, Christine I. Rafie, Panagiotis I. Vlantis, Svetlana Ristin, Miguel A. Gallardo, Supipi L. Auwardt, Natasha K. Khatwani, Yuan-De Tan, Yuguang Ban, et al.pH 7.5,Sigma-Aldrich,Cat#10812846001 Tween-20,Sigma-Aldrich,Cat#11332465001 Digitonin,Promega,Cat#G9441 NEBNext High-Fidelity 2X PCR Master Mix,New England Biolabs,Cat#NC0373959 Histopaque®-1083,Sigma-Aldrich,Cat#

(3) ATM Deficiency Induces TGFβ-Mediated Stromal Programming in Pancreatic CancerCancer ResMay 8, 2026Elodie Roger, Hannah M. Mummey, Eleni Zimmer, Dharini Srinivasan, Anna Härle, Loïc Moubri, Alica K. Michels, Rima Singh, Menar Ekizce, Michael K. Melzer, et al.Tween-20 (Sigma-Aldrich, P7949-100ML), 0.1% IGEPAL (Sigma-Aldrich, I8896), 0.01% digitonin (Promega, G9441), 1 mmol/L DTT (Sigma-Aldrich, D9779), 1 U/μL recombinant RNasin (Promega, PAN2515), 3 mmol/L MgCl2,

(4) Widespread low-affinity motifs enhance chromatin accessibility and regulatory potential in mESCsbioRxivMay 5, 2026Melanie Weilert, Kaelan J. Brennan, Khyati Dalal, Sabrina Krueger, Haining Jiang, Rosa Martinez-Corral, Julia ZeitlingerNaCl, 3 mM MgCl2) supplemented with 0.1% IGEPAL CA-630, 0.1% Tween-20 and 0.01% Digitonin (Promega, G9441). After a 3 min incubation on ice, 1 mL of cold ATAC Resuspension Buffer supplemented with 0.1% Tween-20

(5) Spatially decoding genotype-associated epigenetic landscapes in human lymphoma FFPE tissues via epi-Patho-DBiTNature CommunicationsMay 1, 2026Haikuo Li, Bo Tao, Archibald Enninful, Dingyao Zhang, Yi Dai, Fiona Oh, Negin Farzad, Keyi Li, Zhiliang Bai, Xiaoyu Qin, et al.11332465001), 0.01% NP-40 (Thermo Scientific, cat. no. 28324), 0.001% digitonin (Promega, cat. no. G9441), 1% BSA] for 15 minutes, followed by a 5-min wash with 500 μL of wash buffer (10 mM Tris-HCl pH 7.4,

(6) PTEN restrains SHH medulloblastoma growth through cell autonomous and nonautonomous mechanismseLifeApril 17, 2026Lao Z, El Nagar S, Liang Y, Stephen D, Joyner AL.compound, drug",Tamoxifen,Sigma Aldrich,Cat# T5648-1G, "Chemical compound, drug",Digitonin,Promega,Cat# G9441, "Chemical compound, drug",EdU,Invitrogen,Cat# E10187, "Chemical compound, drug",BrdU,Sigma-Aldrich,Cat#B9285-250MG, "Chemical

(7) PTEN restrains SHH medulloblastoma growth through cell autonomous and nonautonomous mechanismseLifeApril 17, 2026Zhimin Lao, Salsabiel El Nagar, Yinwen Liang, Daniel N Stephen, Alexandra L Joynercompound, drug",Tamoxifen,Sigma Aldrich,Cat# T5648-1G, "Chemical compound, drug",Digitonin,Promega,Cat# G9441, "Chemical compound, drug",EdU,Invitrogen,Cat# E10187, "Chemical compound, drug",BrdU,Sigma-Aldrich,Cat#B9285-250MG, "Chemical

(8) Innate Immune Memory is Stimulus SpecificbioRxivApril 11, 2026Aoife O’Farrell, Zijian Niu, Jingxin Li, Laura C. Van Eyndhoven, Kavitha Sarma, Arjun RajClean and Concentrator Kit,Zymo,D4014 ATACseq Primers,IDT,See Supplemental Table 3 Digitonin,Promega,G9441 Tween20,BioRad,1706531 NP40,Fisher,AAJ19628AP NEBNext HiFi 2x PCR Mix,NEB,M0541L AMPure XP Magnetic

(9) Intestinal Stem Cells From Patients With Inflammatory Bowel Disease Retain an Epigenetic Memory of InflammationCellular and Molecular Gastroenterology and HepatologyMarch 27, 2026Feda H. Hamdan, Mona Farhadipour, Isaiah Perez, Kimberlee Kossick, Hannah Smith, Adam Edwinson, Jose M. de Hoyos-Vega, Michelle Gonzalez, David Chiang, Emily Klatt, et al.for tagmentation for 30 minutes at 37°C in 1X Tagment DNA Buffer, 0.02% Digitonin (Promega, cat no. G9441), 0.2% Tween-20. DNA was extracted using MinElute kit (Qiagen) and amplified using NEBNext High-Fidelity

(10) Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fateNucleic Acids ResMarch 19, 2026Patrizia Maria Christiane Nothnagel, Paul-Arthur Meslin, Jonas Aakre Wik, Damien Dufour, Yunna Erika Strøm, Magnar Bjørås, Jorrit Martijn Enserink, Bjørn Steen Skålhegg, Nolwenn Briand, Anthony Mathelier, et al.pH 7.5, 10 mM NaCl, 3 mM MgCl2 (Sigma, M1028), 0.1% NP-40, 0.1% tween 20, 0.01% Digitonin (Promega, #G9441)] and incubated on ice for 3 min. The nuclei were then washed with the ATAC wash buffer (10 mM Tris–HCl,

(11) Single cell multiomic analysis of the impact of Delta-9-tetrahydrocannabinol on HIV infected CD4 T cellsJournal of Cannabis ResearchMarch 5, 2026Manickam Ashokkumar, Renee Y. Ge, Alicia Cooper-Volkheimer, David M. Margolis, Quefeng Li, Yuchao Jiang, David M. Murdoch, Edward P. Browne655205-250ML), 0.01% nonidet P40 substitute (Sigma, cat. no. I8896), 0.01% Digitonin (Promega, cat. no. G9441), 1 U/μl RiboLock RNase inhibitor, 1% BSA and 1 mM DTT) and incubated on ice for 3 min. The released

(12) Sexual dimorphism in the cell number of the adult Drosophila brainPLOS OneFebruary 18, 2026Narendra Pratap Singh, Susan T. HarbisonATAC-RSBT1 was replaced by 500 μl of ATAC-RSBT2 (RSBT1 + 0.1% NP40, 0.1% Tween-20, and 0.01% Digitonin (G9441, Promega, Madison, USA) added on the day used). The brains were then transferred to a 2 ml Dounce homogenizer

(13) A developmentally regulated long-range enhancer-promoter contact mediates human neural developmentbioRxivFebruary 7, 2026Devin Bready, Shuai Wang, Niklas Ravn-Boess, Joshua Frenster, Jonathan Sabio, Robert Kushmakov, Finnegan Clark, Adler Guerrero, Cathryn Lapierre, Kristyn Galbraith, et al.Genomics,A510008, KAPA Hyperprep Kit,Roche,07962347001, 20% Methanol,Tousimis Research,1008A, Digitonin,Promega,G9441, T4 DNA Ligase,NEB,M0202T, 2X TD buffer,Illumina,20034198, Illumina Tagment DNA Enzyme,Illumina,20034198, MinElute

(14) Manipulation of Alternative Splicing of IKZF1 Elicits Distinct Gene Regulatory Responses in T CellsCellsJanuary 24, 2026Lucia Pastor, Jeremy R. B. Newman, Colin M. Callahan, Rebecca R. Pickin, Mark A. Atkinson, Suna Onengut-Gumuscu, Patrick Concannon(Sigma-Aldrich, Burlington, MA, USA, Cat# 11332465001), and 0.01% Digitonin (Promega, Madison, WI, USA, Cat# G9441). After pelleting the cells, a transposition reaction was performed using Nextera DNA Library Prep Kit

(15) MuMu: a sample multiplexing protocol for droplet-based simultaneous single nuclei RNA- and ATAC-seq systemsbioRxivJanuary 16, 2026Zhen Li, Tarik F. Haydardetergenta,Abcam,ab285400 Tween-20 detergentb,Millipore-Sigma,11332465001 Digitonin detergentc,Promega,G9441 Protector RNase Inhibitor,Millipore-Sigma,3335399001 Critical commercial assays,Critical commercial

(16) Multiomics Profiling of T-cell Leukemia and Lymphoma Enables Targeted Therapeutic DiscoveryCancer ResearchJanuary 16, 2026Aleksandr Ianevski, Kristen Nader, Julia Nguyen, Helena Sorger, Sanna Timonen, Edith Julia, Daniel Pölöske, Katrin Spirk, Christina Wagner, Dennis Jungherz, et al.buffer, 2.5 μL of Tn5 enzyme (Nextera DNA Library Preparation Kit, Illumina), 0.5 μL of 1% digitonin (#G9441, Promega), and 22 μL of nuclease-free water, as previously described (31). The reaction was incubated

(17) Single‐Nucleus Multi‐Omics Reveals Hypoxia‐Driven Angiogenic Programs and Their Epigenetic Control in Sinonasal Squamous Cell CarcinomaAdvanced ScienceJanuary 7, 2026Chaelin You, Jaewoo Park, Jung Yeon Jang, Junho Noh, Jaehyun Lee, Geunho Kwon, Myeong Sang Yu, Yoo‐Sam Chung, Seung‐Jun Lee, Keunsoo Kang, et al.11332473001), 0.1% Tween‐20 (Roche, Catalog no. 11332465001), and 0.01% digitonin (Promega, Catalog no. G9441). This cell lysis reaction was incubated on ice for 3 min, followed by the addition of 1 mL of RSB with

(18) Single cell and spatial characterization of the human pancreas reveals drivers of beta cell dysfunction in cystic fibrosisbioRxivDecember 16, 2025Hannah M. Mummey, Sierra Corban, Jacinta Lucero, Rebecca L. Melton, Madeleine Pittigher, Victoria C. Johnston, Gail H. Deutsch, Agnieszka D'Antonio-Chronowska, Allen Wang, Rebecca L. Hull-Meichle, et al.[AM9530G], 0.01% IGEPAL CA-630 [Sigma I8896], 0.01% Tween-20 [Sigma P7949], 0.001% digitonin [Promega G9441], 1 U/μL RNasin [N2515], and 1× Roche Complete, EDTA-free Protease Inhibitor Cocktail [5056489001].

(19) Protocol for transcriptomic and epigenomic analysis of JAK inhibitor sensitivity in IFN-γ-primed human macrophages using ATAC-seq and RNA-seqSTAR ProtocolsDecember 1, 2025Geunho Kwon, Junho Noh, Jaehyun Lee, Keunsoo Kang, Kyuho Kang15250061 "Tofacitinib citrate, JAK inhibitor",Sigma-Aldrich,Cat# PZ0017-5MG Digitonin,Promega,Cat# G9441 NP40,Roche,Cat# 11332473001 Tween 20,Roche,Cat# 11332465001 "1 M Tris-HCl, pH 7.5",Invitrogen,Cat#

(20) Gene regulatory network inference with popInfer reveals the dynamic regulation of hematopoietic stem cell quiescenceiScienceNovember 11, 2025Megan K. Rommelfanger, Marthe Behrends, Yulin Chen, Jonathan Martinez, Nikith Kurella, Nino Geisler, Deepthi Guturu, Martin Bens, Lingyun Xiong, Zijin Xiang, et al.7.4,Roth,4855.7 NaCl,Roth,3957.5 MgCl2,Merck,814733 Nonidet P40 Substitute,Roche,11332473001 Digitonin,Promega,G9441 Tween 20,Roche,11332465001 ,, Critical commercial assays,Critical commercial assays,Critical commercial

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Digitonin

Digitonin from Promega

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Promega

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Digitonin from Promega

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