Description
Recombinant human RNF34 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The RNF34 gene accession number is NM_025126.
E3 ubiquitin-protein ligase RNF34, also named CARP1 (Caspases-8/10 Associated RING protein 1), acts as RING?domain E3 ligases, ubiquitinates apical caspases and targets them for proteasome-mediated degradation. CARP gene silencing inhibits tumor cell survival and increases cancer cell sensitivity to the death ligand or chemotherapy-induced apoptosis. CARP proteins target the tumor suppressor p53 for degradation. RNF34 is also a negative regulator of the NOD1 pathway through direct interaction and ubiquitination of NOD1