Fig 1: Wnt5a was closely related to abnormal expression of Collagen type II in chondrocytes. (A) The western blotting and (B) qRT-PCR results validated the interactions between Collagen type II and different concentrations of Wnt5a. * represents P < .05; ** represents P < .01; *** represents P < .001, respectively. RT-PCR = reverse transcription-polymerase chain reaction.
Fig 2: Differential expression for Wnt5a and MMP-13 in chondrocytes. The RNA expression levels (A) and protein activities (B) of Wnt5a as well as MMP-13 compared control individuals and OA patients. * represents P < .05; ** represents P < .01; *** represents P < .001, respectively. MMP = matrix metalloproteinase.
Fig 3: Wnt5a was associated with inhibited cell proliferation and upregulated apoptosis in chondrocytes. High expression of Wnt5a were correlated with enhanced cell apoptosis (A) and reduced cell proliferation of chondrocytes (B). * represents P < .05; ** represents P < .01; *** represents P < .001, respectively.
Fig 4: MMP-13 was a direct target for Wnt5a in OA development. The expression level of MMP-13 was examined using western blotting (A). The cell apoptosis (B) and proliferation variability (C) were observed using co-treatment of Wnt5a and MMP-13 siRNA. * represents P < .05; ** represents P < .01; *** represents P < .001 respectively. MMP = matrix metalloproteinase.
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