SDS-PAGE of purified human alpha synuclein S129A monomer (SPR-505) on a 12% Bis-Tris Gel. 2ug and 4ug of total protein were loaded into the respective lanes. Electrophoresis was run at 200V for 45 minutes with fixed voltage using 1X MES running buffer.
Seeding activity of human alpha synuclein S129A mutant measured by ThT in vitro. Human alpha synuclein S129A monomers (SPR-505) are rapidly seeded by both wild-type and S129A alpha synuclein pre-formed fibrils (SPR-506) with activity comparable to wild-type monomers.
Supplier Page from StressMarq Biosciences Inc for Alpha Synuclein S129A Mutant Monomers