Collagenase, Type 1 LS004194 from Worthington Biochemical Corporation

Product Specs
ItemCollagenase, Type 1
CompanyWorthington Biochemical Corporation
Price $42.00Supplier Page View Company Product Page
Catalog NumberLS004194
Source/Expression SystemClostridium histolyticum
StorageStore at 2-8°C
Activity≥125 units per mg dry weight
Size100 mg

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) Functional human salivary gland organoids for tissue regeneration in chemically defined culture systemsCell Reports MedicineFebruary 17, 2026Xinyuan Zhang, Yingyue Chai, Jiaxuan Qiu, Xiaofeng Shan, Shang Xie, Yi Sui, Zhigang Caiproteins","Chemicals, peptides, and recombinant proteins" ,, Collagenase Type I,Worthington Biochemical,Cat#LS004194 Collagenase Type II,Worthington Biochemical,Cat#LS004176 Collagenase Type IV,Worthington Biochemical,Cat#LS004186 A83-01,Sigma-Aldrich,Cat#SML0788 Recombinant

(2) In vivo detection of immune responses via cytokine activity labelingCellFebruary 5, 2026Guangqing Lu, Shanshan Zhang, Mengyang Feng, Eunha Kim, Daniel Cho, Jae Hyun Kim, Hannah Caris, Lev Silberstein, Gloria B. Choi, Jun R. HuhH6278 Bovine Serum Albumin,Millopore-Sigma,Cat# A3059–100G "Collagenase, Type 1",Worthington Biochemical,Cat# LS004194 "DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride)",Thermo Fisher Scientific,Cat# D1306 DMEM (Dulbecco’s

(3) B-1a cells mitigate radiation injury by protecting intestinal barrier integrityFrontiers in ImmunologyFebruary 4, 2026Tomoki Abe, Atsushi Murao, Satoshi Yamaga, Ping Wang, Monowar Azizwere incubated in RPMI 1640 medium supplemented with 2% FBS and 1 mg/mL Collagenase type I (Cat No. LS004194, Worthington Biochemical Corporation, Lakewood, NJ) for 30 min at 37°C with gentle agitation to isolate

(4) Nutrient requirements of organ-specific metastasis in breast cancerNatureJanuary 29, 2026Keene L. Abbott, Sonu Subudhi, Raphael Ferreira, Yetiş Gültekin, Sophie C. Steinbuch, Muhammad Bin Munim, Diya L. Ramesh, Sophie E. Honeder, Ashwin S. Kumar, Michelle Wu, et al.placed directly into 15-ml conical tubes containing 5 ml of digestion buffer (1 mg ml−1 collagenase I (LS004194, Worthington Biochemical), 3 mg ml−1 dispase II (04942078001, Roche) and 0.1 mg ml−1 DNase I (D4527,

(5) Association of Macrophage Accumulation and Polarization in Patients with Obesity and Diabetes with Diabetic Remission After Bariatric SurgeryObesity SurgeryDecember 22, 2025Sa-Hong Kim, Ji-Soo Kim, Jaeun Yoo, Kyoungyun Jeong, Jeesun Kim, Yo-Seok Cho, Ji-Hyeon Park, Jaemoon Koh, Seong-Ho Kong, Do-Joong Park, et al.collagenase buffer (RPMI with collagenase Type 1, Worthington Biochemical Corporation, USA; Cat. No. LS004194) in a shaking incubator (150 RPM, 60 min, 37 °C). The collagen-digested mixture was filtered and centrifuged

(6) Microglial Extracellular Vesicles Mediate C1q Deposition at the Pre-Synapse and Promote Synaptic PruningJ Extracell VesiclesDecember 15, 2025Giulia D'Arrigo, Giulia Cutugno, Maria Teresa Golia, Francesca Sironi, Marta Lombardi, Sara Francesca Colombo, Roberto Frigerio, Marina Cretich, Paola Gagni, Elisabetta Battocchio, et al.cut into small pieces (1–2 mm3) in 1 mL Hibernate‐A on ice, added to a 75 U/mL Collagenase type I (#LS004194, Worthington Biochemical Corporation) Hibernate‐A solution and incubated at 37°C for 15 min. After incubation,

(7) circ_0103896/miR-432–5p/FTO feedback loop suppresses the formation and progression of intracranial aneurysmNoncoding RNA ResDecember 15, 2025Zhiwen Lu, Shijie Zhu, Yina Wu, Fengfeng Xu, Qinghai HuangAfter cleaning, the aortas were placed in 200 μL of DMEM containing 3.33 mg/mL collagenase type I (LS004194, Worthington Biochemical) and incubated at 37 °C for 5 min. The adventitia was then removed, and the

(8) Human CD29+/CD56+ myogenic progenitors display tenogenic differentiation potential and facilitate tendon regenerationElifeMay 19, 2025Xiexiang Shao, Xingzuan Lin, Hao Zhou, Minghui Wang, Lili Han, Xin Fu, Sheng Li, Siyuan Zhu, Shenao Zhou, Wenjun Yang, et al.were minced to 1 mm3 pieces and digested with 3 mg/ml collagenase I (Worthington Biochemical, cat# LS004194) in DMEM (Gibco, cat# 11965118) at 37℃ for 3 hr with gentle agitation. The digested tissue was filtered

(9) R and S enantiomers of CBD3063, a CaV2.2 N-type calcium channel modulator, alleviate capsaicin-induced inflammatory painNeurobiol PainMay 16, 2025Santiago Loya-López, Erick J. Rodríguez-Palma, Aida Calderón-Rivera, Kimberly Gomez, Samantha Perez-Miller, Rajesh KhannaNo. 11965, Thermo Fisher Scientific, Waltham, MA) containing collagenase type I (1.66 mg/mL; Cat. No. LS004194, Worthington Biochemical, Lakewood, NJ) and neural protease (1.04 mg/mL; Cat. No. LS02104, Worthington Biochemical

(10) MIRO1 Is Required for Dynamic Increases in Mitochondria-ER Contact Sites and Mitochondrial ATP During the Cell CycleCellsMarch 22, 2025Benney T. Endoni, Olha M. Koval, Chantal Allamargot, Tara Kortlever, Lan Qian, Riley J. Sadoski, Denise Juhr, Isabella M. Grumbach(1%; GIBCO, Cat# 15140-122), and collagenase I (1 mg/mL; Worthington Biochemical Corporation, Cat# LS004194) at 37 °C in the presence of 5% CO2. The following day, the digested tissue was gently agitated by pipetting

(11) Human immune organoids to decode B cell response in healthy donors and patients with lymphomaNature materialsFebruary 1, 2025Zhong Z, Quiñones-Pérez M, Dai Z, Juarez VM, Bhatia E, Carlson CR, Shah SB, Patel A, Fang Z, Hu T, et al.washed with PBS and enzymatically digested in 150 U ml−1 type I collagenase (Worthington Biochemical, LS004194) for 1 h at 37 °C. Buffer containing serum was then added to stop the enzymatic activity. Organoid debris

(12) T Cells Spatially Regulate B Cell Receptor Signaling in Lymphomas through H3K9me3 ModificationsAdvanced healthcare materialsFebruary 1, 2025Britto LS, Balasubramani D, Desai S, Phillips P, Trehan N, Cesarman E, Koff JL, Singh A.PBS and incubated for 1 h at 37 °C with type‐I collagenase (Worthington Biochemical, catalogue number LS004194) for enzymatic digestion. Organoids were then mechanically dissociated and debris was filtered using

(13) Targeting Nav1.7 and Nav1.8 with a PIKfyve inhibitor to reverse inflammatory and neuropathic painNeurobiology of PainJanuary 1, 2025Erick J. Rodríguez-Palma, Santiago Loya-Lopez, Sophia M. Min, Aida Calderon-Rivera, Kimberly Gomez, Rajesh Khanna, Alison D. AxtmanNo. 11965, Thermo Fisher Scientific, Waltham, MA) containing collagenase type I (1.66 mg/mL; Cat. No. LS004194, Worthington Biochemical, Lakewood, NJ) and neural protease (1.04 mg/mL; Cat. No. LS02104, Worthington Biochemical

(14) Matrix stiffness regulates the triad communication of adipocytes/macrophages/endothelial cells through CXCL13Journal of Lipid ResearchSeptember 1, 2024Arthur Choisez, Seiichiro Ishihara, Takuro Ishii, Yidan Xu, Sepideh D. Firouzjah, Hisashi Haga, Ryoichi Nagatomi, Joji Kusuyamafor 1 h with gentle agitation by inversion every 10–15 min. Subsequently, 2.5 mg collagenase type I (LS004194, Worthington Biochemical) was added to the cell suspension and incubated at 37°C for 20 min with gentle

(15) Effect of Chicken Age on Proliferation and Differentiation Abilities of Muscle Stem Cells and Nutritional Characteristics of Cultured Meat TissueFood Science of Animal ResourcesSeptember 1, 2024Chan-Jin Kim, So-Hee Kim, Eun-Yeong Lee, Young-Hwa Hwang, Seung-Yun Lee, Seon-Tea Jooand dissected into small pieces (~1 mm). These pieces were then treated with 0.1% collagenase type 1 (LS004194, Worthington Biochemical, Lakewood, NJ, USA) at 37°C for 1 h with gentle shaking every 15 min. Dissociated

(16) Cancer tissue of origin constrains the growth and metabolism of metastasesNature MetabolismAugust 19, 2024Sharanya Sivanand, Yetis Gultekin, Peter S. Winter, Sidney Y. Vermeulen, Konstantine M. Tchourine, Keene L. Abbott, Laura V. Danai, Florian Gourgue, Brian T. Do, Kayla Crowder, et al.dissected, minced, and digested at 37°C for 30 min with 1 mg/mL Collagenase I (Worthington Biochemical, LS004194), 3 mg/mL Dispase II (Roche, 04942078001), and 0.1 mg/mL DNase I (Sigma, D4527) in 5 mL PBS. After 30

(17) NAD(+) metabolism enzyme NNMT in cancer-associated fibroblasts drives tumor progression and resistance to immunotherapy by modulating macrophages in urothelial bladder cancerJournal for immunotherapy of cancerJuly 27, 2024Yang M, Wang B, Hou W, Zeng H, He W, Zhang XK, Yan D, Yu H, Huang L, Pei L, et al.minced with a scalpel and digested with a collagenase mixture, containing collagenases (collagenase I, LS004194, 0.17 mg/mL, collagenase II, LS004174, 0.056 mg/mL, collagenase IV, LS004186, 0.17 mg/mL, Worthington Biochemical Corporation

(18) Priming with LSD1 inhibitors promotes the persistence and antitumor effect of adoptively transferred T cellsNature communicationsMay 21, 2024Qiu F, Jiang P, Zhang G, An J, Ruan K, Lyu X, Zhou J, Sheng W.pieces in RPMI1640 medium with the addition of type I collagenase (Worthington Biochemical Corporation, LS004194) and DNase I (Sigma-Aldrich, 10104159001). Tumor tissues were then digested at 37 °C for 20–30 min and

(19) Endothelial-Specific Reduction in Arf6 Impairs Insulin-Stimulated Vasodilation and Skeletal Muscle Blood Flow Resulting in Systemic Insulin Resistance in MiceArteriosclerosis, Thrombosis, and Vascular BiologyMay 1, 2024Md Torikul Islam, Jinjin Cai, Shanena Allen, Denisse G. Moreno, Samuel I. Bloom, R. Colton Bramwell, Jonathan Mitton, Andrew G. Horn, Weiquan Zhu, Anthony J. Donato, et al.were collected, minced using sterile scissors, digested by collagenase-I (Worthington Biochemical Co/ LS004194) and filtered through a 70μm strainer. Cell suspensions were incubated with PECAM conjugated dynabeads.

(20) ZFP541 and KCTD19 regulate chromatin organization and transcription programs for male meiotic progressionCell proliferationApril 1, 2024Zhou X, Fang K, Liu Y, Li W, Tan Y, Zhang J, Yu X, Wang G, Zhang Y, Shang Y, et al.single cells using hyaluronidase (Sigma, H3506), collagenase (Worthington Biochemical Corporation, LS004194), 0.25% trypsin(KeyGEN BioTECH, KGA25200) and DNaseI(Solarbio, D8071). Pachytene spermatocytes were

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Collagenase, Type 1 from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Collagenase, Type 1 from Worthington Biochemical Corporation

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