Human galectin-3 does not bind glycosylated CCL5, and lactose, a sugar known to inhibit glycan/galectin-3 binding, has no effect. CCL5 (OriGene TP303799) was incubated with different ratios of galectin-3-coated beads in the presence or absence of 100 mM lactose, and a bioplex assay measured CCL5 O-glycosylation in the supernatant. Figure cited from Nat Commun, PMID: 28986561
Coomassie blue staining of purified CCL5 protein (Cat# TP303799). The protein was produced from HEK293T cells transfected with CCL5 cDNA clone (Cat# RC203799) using MegaTran 2.0 (Cat# TT210002).
Supplier Page from OriGene Technologies for RANTES (CCL5) (NM_002985) Human Recombinant Protein