Description
IL-4 (Interleukin 4, B cell stimulatory factor) is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. IL-4 has many different functions including promoting proliferation and differentiation of immunologically competent cells. One of its main functions is regulating differentiation of naive CD4+ T cells into helper Th2 cells, the other main function is regulating B cells IgE and IgG1 production. Human IL-4 shows 50% homology with mouse IL-4, but its activities are species-specific and human IL-4 shows no activity on murine and rat cells. Recombinant human IL-4 produced in plant contains 130 amino acids and a 16 a.a. histidine- based tag for a total length of 146 a.a., and has a predicted molecular mass of 17.2 kDa. The recombinant protein migrates as two bands with an apparent molecular mass of 23 kDa and 24 kDa in SDS-PAGE. recombinant human IL-4 is produced in the endosperm tissue of barley grain (Hordeum vulgare), that exhibits up to 50 times less protease activity than E.coli or mammalian cells. Barley seed is void of any human or animal viral contaminants that could jeopardize your cell culture