Native Mouse EGF Protein 53003-018 from Thermo Fisher Scientific

Product Specs
ItemNative Mouse EGF Protein
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog Number53003-018
Storage-20°C
NCBI Full Gene Nameepidermal growth factor
Molecule NameMouse EGF
HostMouse
SpeciesMouse
TypeNative
Formatno preservative
ApplicationsControl, ELISA, Functional assay, Immunohistochemistry, Western Blot
Size100 µg

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Thermo Fisher Scientific

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(1) Decreased PTGES2 Farnesylation in Granulosa Cells Compromises PGE2 ‐Dependent Cumulus Expansion and Oocyte Maturation During Ovarian AgingAging CellFebruary 1, 2026Sainan Zhang, Jiahui Qi, Chuanming Liu, Huidan Zhang, Bichun Guo, Die Wu, Yicen Liu, Xin Zhen, Yang Zhang, Nannan Kang, et al.medium consisting of 10% fetal bovine serum (Gibco, 10270106), 10 ng/mL epidermal growth factor (Gibco, 53003‐018), and 1.5 IU/mL human chorionic gonadotropin (Sansheng Pharmaceuticals, China) with liquid paraffin

(2) IL-25-induced memory type 2 innate lymphoid cells enforce mucosal immunityCellOctober 30, 2025Victor S. Cortez, Sara Viragova, Satoshi Koga, Meizi Liu, Claire E. O’Leary, Roberto R. Ricardo-Gonzalez, Andrew W. Schroeder, Nathan Kochhar, Dedeepya Vaka, Dario Boffelli, et al.,R&D Systems,Cat# 3626-ML-010/CF Recombinant mouse IL-4,Peprotech,Cat# 214-14 Mouse EGF Native Protein,Gibco,Cat# 53003-018 Recombinant Human Noggin Protein,R&D Systems,Cat# 6057-NG 3dGRO® R-Spondin-1 Conditioned Media Supplement,Millipore

(3) Anti-tumor effects on tumor-infiltrating natural killer cells by localized ablative immunotherapy and immune checkpoint inhibitors: An integrated and comparative study using scRNAseq analysisCancer LettersSeptember 1, 2025Kaili Liu, Ashley R. Hoover, Yuanhong Sun, Trisha I. Valerio, Coline Furrer, Jacob Adams, Lin Wang, Abdul Rafeh Naqash, Wei R. Chen(Sigma Aldrich, H0888–10G), 10 ng/mL of mouse epidermal growth factor (EGF) (ThermoFisher Scientific, 53003018), and 50 μg/mL of gentamicin (Genesee Scientific, 25–533). Following incubation, the cells were rinsed

(4) Mevalonate metabolites boost aged oocyte quality through prenylation of small GTPasesNature AgingAugust 26, 2025Chuanming Liu, Huidan Zhang, Jialian Mao, Sainan Zhang, Xiao Tian, Yibing Zhu, Changjiang Wang, Junshun Fang, Huijie Pan, Nannan Kang, et al.medium contained 10% fetal bovine serum (Gibco, 10270106), 10 ng ml−1 epidermal growth factor (Gibco, 53003-018) and 1.5 IU ml−1 human chorionic gonadotropin (Sansheng Pharmaceutical). After culturing in IVM medium

(5) Connecting chemical structure to single cell signaling profilesCommunications BiologyJuly 31, 2025Hannah L. Thirman, Madeline J. Grider-Hayes, Laura C. Geben, Rebecca A. Ihrie, Lauren E. Brown, John A. Porco, Jonathan M. Irishwithout vitamin A (ThermoFisher, 12587010); 20 ng mL−1 mouse epidermal growth factor (ThermoFisher, 53003018); 10 ng mL−1 mouse basic fibroblast growth factor (ThermoFisher, PMG0035); 1 U/mL heparin (Sigma, 9041-08-01);

(6) MyD88-mediated signaling in intestinal fibroblasts regulates macrophage antimicrobial defense and prevents dysbiosis in the gutCell ReportsMay 27, 2025Marina Chulkina, Hanh Tran, Gabriela Uribe, Steven Bruce McAninch, Christina McAninch, Ashley Seideneck, Bing He, Matthew Lanza, Kamil Khanipov, Georgiy Golovko, et al.recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, Mouse EGF Native Protein,Gibco,Cat#53003-018 Accutase,Gibco,Cat#A11105-01 DMEM/F-12 PLUS Basal Medium,Sigma,VSCM162 Gentamicin,Gibco,Cat#15750060 Tamoxifen,Sigma,Cat#T5648-5G Corn

(7) Integer topological defects offer a methodology to quantify and classify active cell monolayersNat CommunMarch 12, 2025Zihui Zhao, He Li, Yisong Yao, Yongfeng Zhao, Francesca Serra, Kyogo Kawaguchi, Hepeng Zhang, Masaki Sanofibroblast growth factor (20 ng/mL, Wako, 060-04543), epidermal growth factor (20 ng/mL, Thermo Fisher, 53003-018), and N-2 Max (100x, R&D Systems, AR009). 35-mm dishes were precoated with 10x diluted Matrigel (Corning,

(8) Active Nematics Reinforce the Ratchet Flow in Dense Environments Without JammingAdvanced ScienceJanuary 23, 2025Yisong Yao, Zihui Zhao, He Li, Yongfeng Zhao, H. P. Zhang, Masaki Sano11039021) was used as medium base supplemented with FGF‐basic (Wako, 060‐04543), EGF (Thermo Fisher, 53003‐018), and N‐2 Max (R&D Systems, AR009). The 35mm plastic dishes (Falcon, 353001) or glass‐bottom dishes

(9) N‐Dihydrogalactochitosan Drives Conventional and Alternative Activations of STING to Synergize Type I IFN and IL‐1β Productions for Antitumor ImmunityAdvanced Functional MaterialsDecember 16, 2024Ashley R. Hoover, Kaili Liu, Coline Furrer, Samuel Siu Kit Lam, David W. Anderson, Zhijun Zhou, Jingxuan Yang, Chun Fung Wong, Alexandra D. Medcalf, Xiao‐Hong Sun, et al.hydrocortisone (Sigma Aldrich, H0888–10G), 10ng/mL mouse epidermal growth factor (EGF) (ThermoFisher Scientific, 53003018), and 50 ug/mL gentamicin (Genesee Scientific, 25–533). Cells were washed twice with Hepes Buffered

(10) Cyclin-dependent kinase inhibitor p18 regulates lineage transitions of excitatory neurons, astrocytes, and interneurons in the mouse cortexThe EMBO JournalDecember 12, 2024Wonyoung Lee, Byunghee Kang, Hyo-Min Kim, Tsuyoshi Ishida, Minkyung Shin, Misato Iwashita, Masahiro Nitta, Aki Shiraishi, Hiroshi Kiyonari, Koichiro Shimoya, et al.1× N2 plus media supplement (#AR009, R&D Systems), 20 ng/ml bFGF (#060-04543, Wako), 20 ng/ml EGF (#53003-018, Invitrogen)) or astrocyte induction medium (DMEM/F12, 0.5× penicillin/streptomycin, 1× N2 plus media

(11) AMH regulates a mosaic population of AMHR2-positive cells in the ovarian surface epitheliumJournal of Biological ChemistryNovember 1, 2024Elizabeth R. Smith, Dorcus Ye, Shihua Luo, Isaac R.L. Xu, Xiang-Xi Xucontaining LIF/β-ME, 0.2 μg/ml recombinant human TGFβ (BioLegend #580702), 0.5 μg/ml mouse EGF (Invitrogen #53003–018), or 6-days conditioned medium collected from wild type or AMH knockout granulosa cells. To prepare

(12) Chemical composition of anti-microbially active fractions derived from extract of filamentous fungus Keratinophyton Lemmensii including three novel bioactive compoundsScientific ReportsOctober 25, 2024Roman Labuda, Markus Bacher, Thomas Rosenau, Hannes Gratzl, Maria Doppler, Sonja Hager, Doris Marko, Christoph Wiesner, Monika Očková, Nicole Ollinger, et al.solution (21115, Sigma-Aldrich), 2 µg/mL ciprofloxacin (17850-5G-F, Sigma-Aldrich), and 50 ng/mL EGF (53003-018, ThermoFisher Scientific) were added. Cells were passaged every 3–5 days until 80% confluence was reached. Decatenation

(13) Lineage-specific changes in mitochondrial properties during neural stem cell differentiationLife science allianceJuly 1, 2024Soares R, Lourenço DM, Mota IF, Sebastião AM, Xapelli S, Morais VA.17504044 Penicillin-Streptomycin,Thermo Fisher Scientific,Cat# 15140122 EGF,Thermo Fisher Scientific,Cat# 53003018 FGF,Thermo Fisher Scientific,Cat# 13256029 Poly-D-Lysine 100 mg,Sigma-Aldrich,Cat# P7886 Laminin,Sigma-Aldrich,Cat#

(14) HOPS, CORVET and newly-identified Hybrid tethering complexes contribute differentially towards multiple modes of endocytosisScientific reportsOctober 31, 2023Terawaki S, Vasilev F, Moriwaki T, Otomo T.nM of BafA1 for 1 h before stimulation. Cells were stimulated with 100 ng/mL of EGF (Thermo Fisher, #53003-018) for 2 h in the presence or absence of BafA1. After 2 h of incubation, cells were washed with PBS and

(15) Epithelial TNF controls cell differentiation and CFTR activity to maintain intestinal mucin homeostasisThe Journal of clinical investigationOctober 16, 2023Reyes EA, Castillo-Azofeifa D, Rispal J, Wald T, Zwick RK, Palikuqi B, Mujukian A, Rabizadeh S, Gupta AR, Gardner JM, et al.Bisindolylmaleimide I (GF109203X; S7208, Selleckchem) was resuspended in DMSO at a concentration of 10 mM and stored at –80°C. EGF (53003-018, Invitrogen) was resuspended in PBS with 0.1% BSA at a concentration of 50 μg/mL and stored at –20°C.

(16) Dephosphorylation of 4EBP1/2 Induces Prenatal Neural Stem Cell QuiescencebioRxivFebruary 19, 2023Laura C. Geben, Asa A. Brockman, Mary Bronwen L. Chalkley, Serena R. Sweet, Julia E. Gallagher, Alexandra L. Scheuing, Richard B. Simerly, Kevin C. Ess, Jonathan M. Irish, Rebecca A. Ihriesupplement without vitamin A (ThermoFisher, 12587010); 20 ng/mL mouse epidermal growth factor (ThermoFisher, 53003018); 10 ng/mL mouse basic fibroblast growth factor (ThermoFisher, PMG0035); 1 U/mL heparin (Sigma, 9041-08-01);

(17) Polycomb deficiency drives a FOXP2-high aggressive state targetable by epigenetic inhibitorsNature communicationsJanuary 20, 2023Chen F, Byrd AL, Liu J, Flight RM, DuCote TJ, Naughton KJ, Song X, Edgin AR, Lukyanchuk A, Dixon DT, et al.extract (Invitrogen, #13028-014), 0.1 µg/mL cholera toxin (SIGMA, #C8052), 25 ng/mL mEGF (Invitrogen, #53003-018) and 25 ng/mL rmFGF2 (R + D Systems, #3139-FB/CF), mixed 1:1 with growth factor-reduced Matrigel (Corning),

(18) Combined inhibition of EZH2 and ATM is synthetic lethal in BRCA1-deficient breast cancerBreast Cancer ResearchDecember 1, 2022Leonie Ratz et al.FCS, 1% Penicillin–Streptomycin (5000 U/mL, Thermo Fisher, Cat#12140122), 5 mg/mL insulin (Sigma, Cat#53003-018), 5 ng/mL epidermal growth factor (Thermo Fisher, Cat#I6634), and 5 ng/mL cholera toxin (Sigma-Aldrich

(19) Re-purposing the pro-senescence properties of doxorubicin to introduce immunotherapy in breast cancer brain metastasisCell reports. MedicineNovember 15, 2022Uceda-Castro R, Margarido AS, Cornet L, Vegna S, Hahn K, Song JY, Putavet DA, van Geldorp M, Çitirikkaya CH, de Keizer PLJ, et al.(10%, Thermo Fisher Scientific, Cat. No. A4766801), EGF (5 ng/mL, Thermo Fisher Scientific, Cat. No. 53003018) and Insulin (5 μg/mL, Sigma Aldrich, Cat. No. I0516) in 20% O2, 5% CO2 and at 37°C. Method details Intracranial

(20) The kinase PLK1 promotes the development of Kras/Tp53-mutant lung adenocarcinoma through transcriptional activation of the receptor RETScience signalingOctober 4, 2022Kong Y, Allison DB, Zhang Q, He D, Li Y, Mao F, Li C, Li Z, Zhang Y, Wang J, et al.L-glutamine, 12.5μg/mL bovine pituitary extract (Invitrogen, #13028-014), 25 ng/mL mEGF (Invitrogen, #53003-018) and 25 ng/mL rmFGF2 (R+D Systems, #3139-FB/CF) into DMEM/F12 medium. Tumor cells (~2500) were dissociated,

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Native Mouse EGF Protein from Thermo Fisher Scientific

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Native Mouse EGF Protein from Thermo Fisher Scientific

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