Fig 1: The increase of GSK3β activity by Netrin-1. (A) Western blotting showing the activity of kinases as indicated after Netrin-1 stimulation. (B) Quantification of the phosphorylated levels of indicated kinases. Student’s t test, p < 0.01 for pGSK3β. Data are presented as the mean ± SEM. The data are from at least three independent experiments. ns, no significant difference; ** p < 0.01.
Fig 2: The high expression of Integrin α6β1 in microglia as well as the interaction between Integrin α6β1 and Netrin-1. (A) Single-cell data from Tabula Muris showing the expression level of Netrin-1 traditional receptors, involving Dcc, Neo1, Unc5a, Unc5b, Unc5c, Unc5d, and Dscam in mouse microglia. (B) Single-cell data from Tabula Muris showing the expression level of Integrin subunits associated with Netrin-1. (C) Multiple MST traces for different mixture ratios of Integrin α6β1 and Netrin-1. (D) Dose–response analysis of the MST traces for determination of the steady-state affinity of the Integrin α6β1–Netrin-1 interaction.
Fig 3: The decrease of Iba1+ microglia in the developing cerebral cortex of Ntn1 cKO mice. (A) Immunostaining showing the number of Iba1+ microglia in the cortical brain at indicated developmental stages. (B) Quantification of the microglia numbers. For each group, 10 sections from 3 mice were used for statistical analysis. Student’s t test, for E18.5, p < 0.0001; for P5, p < 0.0001; for P14, p = 0.1159; for P30, p = 0.2692; for P30, p = 0.3057. Data are presented as the mean ± SEM. The data are from at least three independent experiments. ns, no significant difference; *** p < 0.001.
Fig 4: Suppression of Netrin-1 mediated BV2 migration by GSK3β inhibitor. (A) Representative images showing BV2 cell morphology after Netrin-1 stimulation with or without LiCl (6 h). (B) Quantification of the percentage of elongated BV2 cells. One-way ANOVA, for Control and Netrin-1 groups, p < 0.0001; for Control and Netrin-1 + LiCl groups, p = 0.3314; for Netrin-1 and Netrin-1 + LiCl groups, p < 0.0001. (C) Trans-well assay showing the migration of BV2 cells after Netrin-1 stimulation with or without LiCl (18 h). (D) Quantification of the number of migrated BV2 cells. One-way ANOVA, for Control and Netrin-1 groups, p < 0.0001; for Control and Netrin-1 + LiCl groups, p = 0.0102; for Netrin-1 and Netrin-1 + LiCl groups, p < 0.0001. The data are from at least three independent experiments. ns, no significant difference; * p < 0.05; *** p < 0.001.
Fig 5: The expression and distribution of Netrin-1 in developing cerebral cortex. (A) Western blotting showing the expression level of Netrin-1 at indicated developmental stages. (B) The distribution of extracellular Netrin-1 in E15.5 brain sections.
Supplier Page from Enzo Life Sciences, Inc. for Netrin-1 (human), (recombinant)