Fig 1: GP treatment relieves depression-like behavior and oxidative stress in CUMS-induced mice. The mice used for experiments were as follows: control mice, CUMS-induced mice and CUMS-induced mice administered with GP25, GP50, or GP100. (A) Sucrose preference of mice detected by SPT. (B) The retention time in open field of mice detected by OFT. (C) The immobility time of mice detected by TST. (D) The immobility time in water of mice detected by FST. (E) The percentage of time mice spent in the target quadrant of the platform detected by MWM. (F) the Weights of mice at the day 0, 14, 28, and 42 of CUMS induction. (G) The serum levels of TNF-a, IL-1ß, and IL-6 in mice detected by ELISA. (H) The serum levels of SOD, MDA, GSH, and CAT in mice detected by ELISA. (I) The hippocampus tissues in mice observed by HE staining. (J,K) Representative views (J) and Nissl positive cell ratio (K) in hippocampus tissues of mice detected by Nissl staining. (L,M) Representative views (L) and apoptotic neuron ratio (M) in hippocampus tissues of mice detected by TUNEL staining. The measurement data were presented as mean ± standard deviation. Data comparison among multiple groups was analyzed by one-way ANOVA, followed by Tukey’s post hoc test. Data at different time points among multiple groups were compared by repeated measures ANOVA, and Bonferroni was applied for post hoc test. n = 15. *p < 0.05 vs. the CUMS-induced mice; #p < 0.05 vs. the control mice.
Fig 2: The regulatory role of GC in the mouse dental caries model. (a) GC inhibited the levels of IL-1β, IL-6 and TNF-α in mouse model (Control vs. Model, 95% CI [−488, −375]; Control vs. Model + Galla, 95% CI [−129, −15.9]; Model vs. Model + Galla, 95% CI [303, 416]). (b) Effect of GC on MALAT1 expression in C57 mouse (Control vs. Model, 95% CI [−0.870, −0.450]; Model vs. Model + Galla, 95% CI [0.500, 0.920]). (c) Effect of GC on miR-181b expression in C57 mouse (Control vs. Model, 95% CI [0.390, 0.737]; Model vs. Model + Galla, 95% CI [−0.653, −0.307]). *** P < 0.001 compared to control; ### P < 0.001 compared to model
Fig 3: Study on the regulatory effects of GC on the HDPSCs cell model. (a) GC enhanced cell viability in LPS cell model. (b) GC inhibited the levels of IL-1β, IL-6 and TNF-α in cell model. (c) Effect of GC on MALAT1 expression in HDPSCs (Control vs. Model, 95% CI [−0.874, −0.306]; Model vs. Model + Galla, 95% CI [0.272, 0.841]). (d) Effect of GC on miR-181b expression in HDPSCs (Control vs. Model, 95% CI [0.367, 0.667]; Model vs. Model + Galla, 95% CI [−0.560, −0.260]). ** P < 0.01, *** P < 0.001 compared to control; ### P < 0.001 compared to model
Supplier Page from Thermo Fisher Scientific for Recombinant Mouse IL-6 (ELISA Standard) Protein