Mouse CD16-2 / FCGR4 Protein (His & AVI Tag), Biotinylated 50036-M27H-B from Sino Biological, Inc.

Product Specs
ItemMouse CD16-2 / FCGR4 Protein (His & AVI Tag), Biotinylated
CompanySino Biological, Inc.
Price $688.00Supplier Page View Company Product Page
Catalog Number50036-M27H-B
Conjugate/TagC-AVI, His
Purity> 95 % as determined by SDS-PAGE
TypeRecombinant protein
Molecular WeightThe recombinant mouse FCGR4 consists of 214 amino acids and has a predicted molecular mass of 24.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFCGR4 is approximately 35-40 kDa due to glycosylation
SpeciesMouse
Molecule NameFCGR4
Source/Expression SystemHEK293 Cells
Activity1.Loaded Recombinant Mouse FCGR4 Protein, His Tag, Biotinylated (Cat. No. 50036-M27H-B) on SA Biosensor, can bind Anti-CD3 (Research Grade OKT3 Biosimilar) with an affinity constant of 22.6nM as determined in BLI assay (Sartorius Octet RED384)(QC tested). 2.Loaded Biotinylated Recombinant Mouse CD16-2/FCGR4/Fc gamma RIV Protein, His & AVI Tag (Cat. No. 50036-M27H-B) on SA Biosensor, can bind Anti-CD20 (Research Grade Rituximab Biosimilar) with an affinity constant of 0.475 µM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 3.Immobilized Anti-CD3 (OKT3) (Research Grade Muromonab Biosimilar) at 5 µg/mL (100 µL/well) can bind Recombinant Mouse CD16-2/FCGR4/Fc gamma RIV Protein (His & AVI Tag), Biotinylated (Cat: 50036-M27H-B) the EC50 is 3.1 ng/mL(Routinely tested)
FormatLyophilized from sterile PBS, pH 7.4
Size100 µg

37 Industrial Blvd

Suite B

Paoli, PA 19301

United States

Phone: 215-583-7898
Fax: 267-657-0217

Email: marketing@sinobiological.com

(1) Systems serology-based comparison of humoral immune responses induced by liposome or aluminum hydroxide adjuvanted SARS-CoV-2 spike proteinSci RepMay 28, 2025Soo Ji Kim, Taewoo Kim, Satyanarayana Bejjani, Mi Sun Kim, Jung Hyuk Lee, Yuna Shin, Sun-Je Woo, Beom Min Cheon, Doyoung Kim, Sumin Lee, et al.FcγR2b (Sino Biological, 50030-M27H-B), FcγR3 (Sino Biological, 50326-M27H-B), FcγR4 (Sino Biological, 50036-M27H-B)] and Streptavidin R Phycoerythrin (Agilent, PJ315) for 1 h at room temperature on a plate shaker at

(2) Biological and structural characterization of murine TRALI antibody reveals increased Fc-mediated complement activationBlood advancesAugust 25, 2020Zeeuw van der Laan EAN, van der Velden S, Bentlage AEH, Larsen MD, van Osch TLJ, Mok JY, Brasser G, Geerdes DM, Koeleman CAM, Nouta J, et al.

(3) FcγR interaction is not required for effective anti-PD-L1 immunotherapy but can add additional benefit depending on the tumor modelInternational journal of cancerJanuary 15, 2019Sow HS, Benonisson H, Breukel C, Visser R, Verhagen OJHM, Bentlage AEH, Brouwers C, Claassens JWC, Linssen MM, Camps M, et al.

(4) Conserved FcγR- glycan discriminates between fucosylated and afucosylated IgG in humans and miceMolecular immunologyFebruary 1, 2018Dekkers G, Bentlage AEH, Plomp R, Visser R, Koeleman CAM, Beentjes A, Mok JY, van Esch WJE, Wuhrer M, Rispens T, et al.

(5) Affinity of human IgG subclasses to mouse Fc gamma receptorsmAbsJuly 1, 2017Dekkers G, Bentlage AEH, Stegmann TC, Howie HL, Lissenberg-Thunnissen S, Zimring J, Rispens T, Vidarsson G.

Mouse CD16-2 / FCGR4 Protein (His & AVI Tag), Biotinylated