Cynomolgus CD32b / FCGR2B Protein (His & AVI Tag), Biotinylated 90014-C27H-B from Sino Biological, Inc.

Description

A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD32b (AAL92097.1) extracellular domain (Met 1-Gly 220) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed

Product Specs
ItemCynomolgus CD32b / FCGR2B Protein (His & AVI Tag), Biotinylated
CompanySino Biological, Inc.
Price $688.00Supplier Page View Company Product Page
Catalog Number90014-C27H-B
Conjugate/TagC-AVI, His
Purity> 97 % as determined by SDS-PAGE
TypeRecombinant protein
Molecular WeightThe recombinant cynomolgus CD32b consists of 211 amino acids and has a calculated molecular mass of 23.5 kDa. The apparent molecular mass of the recombinant protein is approximately 34 kDa in SDS-PAGE under reducing conditions due to glycosylation
SpeciesCynomolgus
Molecule NameFCGR2B
Source/Expression SystemHEK293 Cells
FormatLyophilized from sterile PBS, pH 7.4
Activity1.Immobilized Cynomolgus CD32B/Fcgr2b Protein (His & AVI Tag), Biotinylated (Cat:90014-C27H-B) at 2 µg/mL (100 µL/well) can bind Human IgG2 Protein (257 Ser/Ala) (Cat:13504-HNAH), the EC50 is 4-36 ng/mL. 2.Loaded Recombinant Cynomolgus CD32B / Fcgr2b Protein, His & AVI Tag, Avi-tag Biotinylated (Cat. No. 90014-C27H-B) on SA Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 1.4 µM as determined in BLI assay (Sartorius Octet RED384) (QC tested)
Size100 µg