Human VEGFR2 / Flk-1 / CD309 / KDR Protein (Fc Tag) 10012-H02H from Sino Biological, Inc.

Product Specs
ItemHuman VEGFR2 / Flk-1 / CD309 / KDR Protein (Fc Tag)
CompanySino Biological, Inc.
Price $2,248.00Supplier Page View Company Product Page
Catalog Number10012-H02H
Size1 mg
Conjugate/TagC-human IgG1-Fc
Purity> 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC
TypeRecombinant protein
Molecular WeightThe recombinant human VEGFR2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 979 amino acids and predicts a molecular mass of 109 kDa. The apparent molecular mass of human VEGFR2/Fc monomer is approximatly 150-160 KDa in SDS-PAGE under reducing conditions due to glycosylation
SpeciesHuman
Molecule NameKDR
Source/Expression SystemHEK293 Cells
Activity1. Immobilized Human VEGF121 (Cat:10008-HNAH) at 2 µg/mL (100 µL/well) can bind Human VEGFR2/KDR (ECD, hFc Tag)(Cat:10012-H02H), the EC50 of Human VEGFR2/KDR (ECD, hFc Tag)(Cat:10012-H02H) is 6-48 ng/mL. 2. Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 20-120 ng/mL in the presence of 10 ng/mL recombinant human VEGF165. 3.Recombinant Human VEGFR2 / Flk-1 / CD309 / KDR Protein (Fc Tag) (Cat: 10012-H02H) captured on Protein A chip, can bind Recombinant Human VEGF110 / VEGF-A Protein (Cat: 29714-HNAH) with an affinity constant of 22.800 pM as determined in an SPR assay (Biacore 8K) (Routinely tested)
FormatLyophilized from sterile PBS, pH 7.4