TMB One Component Substrate E102 from Bethyl Laboratories, Inc.

Product Specs
ItemTMB One Component Substrate
CompanyBethyl Laboratories, Inc.
Price $57.00Supplier Page View Company Product Page
Catalog NumberE102
Size120 ml
ApplicationsELISA
Storage2 - 8°C

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Bethyl Laboratories, Inc.

P.O. Box 850

Montgomery, Texas 77356

United States

Phone: 1-800-338-9579
Fax: 1-866-597-6105

Email: orders@fortislife.com

Company Profile

Website: fortislife.com

(1) Local Inflammatory and Systemic Antibody Responses Initiated by a First Intradermal Administration of Autogenous Salmonella-Killed Vaccines and Their Components in PulletsVaccines (Basel)October 11, 2024Jossie M. Santamaria, Chrysta N. Beck, Gisela F. ErfA total of 100 µL of TMB substrate solution (3,3′,5,5′-Tetramethylbenzidine one component substrate E102, Bethyl Laboratories) was added to all wells, and plates were incubated at 37 °C for 15 min. The color

(2) Discovery and Preclinical Characterization of XMT-1660, an Optimized B7-H4-Targeted Antibody-Drug Conjugate for the Treatment of CancerMolecular cancer therapeuticsSeptember 5, 2023Toader D, Fessler SP, Collins SD, Conlon PR, Bollu R, Catcott KC, Chin CN, Dirksen A, Du B, Duvall JR, et al.catalog no. 109–035–097) for 1 hour and washed 3 times. The HRP substrate, TMB (Bethyl Lab, catalog no. E102), was added and incubated for four minutes. The reaction was quenched with sulfuric acid (0.2 N, 100

(3) Enhancement of Molecular Transport into Film Stacked Structures for Micro-Immunoassay by Unsteady RotationMicromachines (Basel)March 28, 2023Hinata Maeno, Satoshi Ogata, Tetsuhide Shimizu, Ming Yangkit (E80-102), ELISA Blocking Buffer (E104), Wash Solution(E106), and TMB One Component Substrate (E102) were purchased from Bethyl Laboratories. Sodium hydroxide (NaOH), 2-(N-morpholino)ethanesulfonic acid

(4) Soluble α-klotho and heparin modulate the pathologic cardiac actions of fibroblast growth factor 23 in chronic kidney diseaseKidney internationalAugust 1, 2022Yanucil C, Kentrup D, Campos I, Czaya B, Heitman K, Westbrook D, Osis G, Grabner A, Wende AR, Vallejo J, et al.with anti-human Fc coupled to HRP at 1:10,000. Plates were washed as above and 100 μL TMB substrate (E102, Bethyl) was added for 15–20 minutes until positive wells developed a dark blue color. Reactions were

(5) FGF21-FGFR4 signaling in cardiac myocytes promotes concentric cardiac hypertrophy in mouse models of diabetesScientific reportsMay 5, 2022Yanucil C, Kentrup D, Li X, Grabner A, Schramm K, Martinez EC, Li J, Campos I, Czaya B, Heitman K, et al.incubated with 100 μL anti-human Fc-HRP at 1:10,000. Plates were washed as above and 100 μL TMB substrate (E102, Bethyl) was added for 15–20 min until positive wells developed a dark blue color. Reactions were stopped

(6) Self-inactivating, all-in-one AAV vectors for precision Cas9 genome editing via homology-directed repair in vivoNature CommunicationsDecember 1, 2021Raed Ibraheim et al.immune responses, the plate was developed by adding 100 μL of TMB One Component HRP Substrate (Bethyl E102). After incubating in the dark for 30 min at room temperature, Stop Solution (Bethyl E115; 100 μL per

(7) Systemic administration of monovalent follistatin-like 3-Fc-fusion protein increases muscle mass in miceiScienceMay 21, 2021Ozawa T, Morikawa M, Morishita Y, Ogikubo K, Itoh F, Koinuma D, Nygren PÅ, Miyazono K.streptavidin (405210; BioLegend) was used to detect ligands for 1 h at room temperature. After TMB (E102; Bethyl Laboratories) and HRP reaction for 10 min in the dark, 0.18 M H2SO4 was added to each well to

(8) Development of a pancreas-liver organ-on-chip coculture model for organ-to-organ interaction studiesBiochemical Engineering JournalDecember 1, 2020Amal Essaouiba, Teru Okitsu, Rie Kinoshita, Rachid Jellali, Marie Shinohara, Mathieu Danoy, Cécile Legallais, Yasuyuki Sakai, Eric Leclercquantification set from Bethyl, Cat. No. E110-125 combined with the Enzyme Substrate, TMB, Cat. No. E102). Results were obtained with an iMark microplate reader (Bio-Rad, Osaka, Japan) set to a wavelength

(9) Comparative study between formalin-killed vaccine and developed gammairradiation vaccine against Mannheimia haemolytica in rabbitsTURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCESJanuary 1, 2016Sahar AHMED, Basem AHMED, Ghada MAHMOUD, Waleed NEMR, Emam ABDEL RAHIMfor 1 h. Next, 100 µL of substrate 3,3,5,5-tetramethylbenzidine solution (Bethyl Laboratories; Cat. No. E102) was added and kept for 15 min at 37 °C, after which a color change was observed in the wells. The reaction

(10) Distribution of Peripheral Memory T Follicular Helper Cells in Patients with Schistosomiasis JaponicaPLoS neglected tropical diseasesJanuary 1, 2015Chen X, Li W, Zhang Y, Song X, Xu L, Xu Z, Zhou S, Zhu J, Jin X, Liu F, et al.in Sample/Conjugate Diluent Solution, followed by the addition of Substrate Solution (catalog number E102, Bethyl). After 15 min, the reaction was stopped with 100 μL of 0.18M sulfuric acid solution. Absorbance

(11) Development of Gamma Irradiation Vaccine against Mannheimia haemolytica: A Preliminary StudyResearch Journal of ImmunologyJanuary 1, 2015Sahar Ahmed, Basem S. Ahmed, Ghada I. Mahmoud, Walid Elnamer, E.A. Abdel-Rahiof the substrate 3, 3, 5, 5, -tetramethyl benzidine (TMB) (Bethyl laboratories. Inc,, USA Cat. No. E102) solution was added and kept for 15 min at 37°C. A color reaction was developed with the wells. The

(12) Cardiac contractile function and mitochondrial respiration in diabetes-related mouse modelsCardiovascular DiabetologyDecember 1, 2014Camille Marciniak, Xavier Marechal, David Montaigne, Remi Neviere, Steve Lancel

(13) Fibronectin-Binding Protein of Borrelia hermsii Expressed in the Blood of Mice with Relapsing FeverInfection and ImmunityJune 1, 2014Eric R. G. Lewis, Renee A. Marcsisin, Shelley A. Campeau Miller, Fong Hue, April Phillips, David P. AuCoin, Alan G. Barbour

(14) Decreased Pattern-Recognition Receptor-Mediated Cytokine mRNA Expression in Obese Children With Otitis Media With EffusionClinical and Experimental OtorhinolaryngologyFebruary 5, 2014Youn Jung Kim, Sung Ho Cha, Ho Yun Lee, Sun Kyu Lee, Hee Yong Chung, Joon Hyung Yeo, Young Il Kim, Seung Geun Yeo

(15) Correction: Hibernating Little Brown Myotis (Myotis lucifugus) Show Variable Immunological Responses to White-Nose SyndromePLoS ONEOctober 4, 2013Marianne S. Moore, Jonathan D. Reichard, Timothy D. Murtha, Morgan L. Nabhan, Rachel E. Pian, Jennifer S. Ferreira, Thomas H. Kunza 1:1 TMB (3, 39, 5, 59 tetramethyl benzidine) and peroxidase substrate (Bethyl Laboratories Inc. #E102). 100 mL of enzyme substrate solution was dispensed into each well, and the plate was incubated at room temperature

(16) Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitroVeterinary researchJuly 22, 2013Ikebuchi R, Konnai S, Okagawa T, Yokoyama K, Nakajima C, Suzuki Y, Murata S, Ohashi K.

(17) Hibernating Little Brown Myotis (Myotis lucifugus) Show Variable Immunological Responses to White-Nose SyndromePLoS ONEMarch 20, 2013Marianne S. Moore, Jonathan D. Reichard, Timothy D. Murtha, Morgan L. Nabhan, Rachel E. Pian, Jennifer S. Ferreira, Thomas H. Kunza 1∶1 TMB (3, 3′, 5, 5′ tetramethyl benzidine) and peroxidase substrate (Bethyl Laboratories Inc. #E102). 100 µL of enzyme substrate solution was dispensed into each well, and the plate was incubated at room

(18) Development of monoclonal antibody-based immunoassays for detection of Helicobacter pylori neutrophil-activating proteinJournal of immunological methodsOctober 31, 2012Iankov ID, Penheiter AR, Carlson SK, Galanis E.

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TMB One Component Substrate from Bethyl Laboratories, Inc.

Bethyl Laboratories, Inc.

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TMB One Component Substrate from Bethyl Laboratories, Inc.

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