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FITC Annexin V
FITC Annexin V from BioLegend
(1)
Write a review
Citations:
(269)
Supplier Page
Supplier Page from
BioLegend for
FITC Annexin V
Description
FITC Annexin V
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
FITC Annexin V
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
640906
Conjugate/Tag
FITC
Applications
Flow Cytometry (FC)
Quantity
100 tests
Gene Name
annexin A5
NCBI Gene Aliases
ANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
NCBI Full Gene Name
annexin A5
Format
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Target
Annexin V
Species
Human, Mouse, Rat
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (269)
(1)
p38 Mitogen-Activated Protein Kinase Inhibition of Mesenchymal Transdifferentiated Tumor Cells in Head and Neck Squamous Cell Carcinoma
Biomedicines
December 13, 2023
Julia Federspiel, Maria do Carmo Greier, Andrea Ladányi, Jozsef Dudas
µL Annexin Assay Buffer (0.1 M HEPES, 0.14 M NaCl, 2.5 mM CaCl2). Five µL Annexin V-FITC (Cat. Nr.
640906
) and 2 µL Podoplanin APC antibody (Cat. Nr.
337021
) (both from
Biolegend
) were added for a 25 min
incubation
(2)
CD70-induced differentiation of proinflammatory Th1/17/22/GM lymphocytes associated with disease progression and immune reconstitution during HIV infection
Emerging Microbes & Infections
December 8, 2023
Xinyue Wang, Yuqing Wei, Zhijiao He, Di Wang, Leidan Zhang, Juan Du, Mengyuan Zhang, Meiqing Jiang, Na Chen, Meiju Deng,
et al.
A15153G, RRID: AB_2632931), CD95-PE-CY594 (
clone
DX2, RRID: AB_11153666), Annexin V-FITC (
BioLegend
, cat#
640906
), and the corresponding isotype controls. For intracellular
staining
of KI67-FITC (
clone
Ki-67, RRID:
(3)
The synthetic oleanane triterpenoid CDDO-2P-Im binds GRP78/BiP to induce unfolded protein response-mediated apoptosis in myeloma
Molecular oncology
December 1, 2023
Luo G, Aldridge K, Chen T, Aslot V, Kim BG, Han EH, Singh N, Li S, Xiao TS, Sporn MB,
et al
.
DMSO (1%) or the specified therapeutic agents for 24 h. Cells were stained with Annexin V (Catalog #
640906
;
Biolegend
, San Diego, CA, USA) and propidium iodide (Catalog #
421301
;
Biolegend
) after 24 h and analyzed
(4)
Viral and host factors drive a type 1 Epstein–Barr virus spontaneous lytic phenotype
mBio
November 16, 2023
Katherine A. Willard, Ashley P. Barry, Cliff I. Oduor, John Michael Ong'echa, Jeffrey A. Bailey, Ann M. Moormann, Micah A. Luftig
,
Santa Cruz
,
sc-81581
LMP1,S12,
EBV
,WB,
Hybridoma
,N/A EBNALP,JF186,
EBV
,WB,
Hybridoma
,N/A
Magoh
,21B12,
Human
,WB,
Santa Cruz
,
sc-56724
Annexin V-FITC,N/A,
Human
,FACS,
BioLegend
,
640906
LMP2A,14B7,
EBV
,WB,
Santa Cruz
,
sc-101314
c-Myc
,9E10,
Human
,WB,
Santa Cruz
,
sc-40
SLAMF7
/
CD319
-FITC,162.1,
Human
,FACS,
BioLegend
,
331817
Ea-D,O261,
EBV
,FACS,
Santa Cruz
(5)
Water Extract of Capsella bursa-pastoris Mitigates Doxorubicin-Induced Cardiotoxicity by Upregulating Antioxidant Enzymes
International Journal of Molecular Sciences
November 2, 2023
Yuhui Jeong, Sun-Ho Lee, Jangho Lee, Min-Sun Kim, Yu-Geon Lee, Jin-Taek Hwang, Sang-Yoon Choi, Ho-Geun Yoon, Tae-Gyu Lim, Seung-Hyun Lee,
et al.
cells were quantitatively analyzed by
flow cytometry
using Annexin V-fluorescein isothiocyanate (FITC) (
640906
,
BioLegend
, Sandiego, CA, USA), following the manufacturer’s protocol. Briefly, H9c2 cells were cultured
(6)
RhoA balances microglial reactivity and survival during neuroinflammation
Cell Death & Disease
October 20, 2023
Renato Socodato, Artur Rodrigues-Santos, Joana Tedim-Moreira, Tiago O. Almeida, Teresa Canedo, Camila C. Portugal, João B. Relvas
BioLegend
, USA) as we did before [24, 92]. Necrotic and apoptotic
microglia
were determined using Annexin V (
640906
BioLegend
, USA) and Zombie
Violet
Dye (77477 BioLegend, USA).
Mice
were anesthetized with sodium pentobarbital
(7)
Arginine dependency is a therapeutically exploitable vulnerability in chronic myeloid leukaemic stem cells
EMBO reports
October 9, 2023
Kevin M Rattigan, Martha M Zarou, Zuzana Brabcova, Bodhayan Prasad, Désirée Zerbst, Daniele Sarnello, Eric R Kalkman, Angela Ianniciello, Mary T Scott, Karen Dunn,
et al.
manufacturer's instructions. Subsequently cells were stained with Annexin V (FITC or APC,
BioLegend
:
640906
or
640941
, 5 μl/test),
7‐AAD
(
BD Bioscience
:
559925
, 5 μl/test) and
CD34
+ (APC,
BD Bioscience
:
555824
,
(8)
Multi-step processing of replication stress-derived nascent strand DNA gaps by MRE11 and EXO1 nucleases
Nature communications
October 7, 2023
Hale A, Dhoonmoon A, Straka J, Nicolae CM, Moldovan GL.
GloMax Navigator plate reader. Apoptosis
assays
were performed using the
FITC Annexin V
kit (
Biolegend
,
640906
). Quantification was performed on a
BD
FACSCanto
10
flow cytometer
using the FlowJo v10 software. DNA
(9)
Ultra‐high static magnetic fields cause immunosuppression through disrupting B‐cell peripheral differentiation and negatively regulating BCR signaling
MedComm (2020)
October 1, 2023
Heng Gu, Yufan Fu, Biao Yu, Li Luo, Danqing Kang, Miaomiao Xie, Yukai Jing, Qiuyue Chen, Xin Zhang, Juan Lai,
et al.
PE‐Cy7‐anti‐ki67 (1983210; Invitrogen) was used to label cells in the proliferation cycle and Annexin V (
640906
;
BioLegend
) was used to detect apoptosis. Data were acquired on
Attune NxT
(
Thermo Fisher
) and analyzed
(10)
Chromatin-Associated SIN3B Protects Cancer Cells from Genotoxic Stress-Induced Apoptosis and Dictates DNA Damage Repair Pathway Choice
Molecular cancer research : MCR
September 1, 2023
Morales-Valencia J, Petit C, Calderon A, Saini S, David G.
resuspended in 200 uL of binding buffer (
BioLegend
,
422201
) along with 5 uL of Annexin V (
BioLegend
,
640906
). Following 30 minutes
incubation
at room temperature and
centrifugation
, the cell pellet resuspended
(11)
Inhibition of VCP modulates NF-κB signaling pathway to suppress multiple myeloma cell proliferation and osteoclast differentiation
Aging (Albany NY)
August 30, 2023
Rongfang Wei, Yuhao Cao, Hongjie Wu, Xin Liu, Mingmei Jiang, Xian Luo, Zhendong Deng, Ze Wang, Mengying Ke, Yongqiang Zhu,
et al.
was purchased from Beyotime (ST511, Shanghai, China), Annexin-V-FITC was purchased from
Biolegend
(
640906
, San Diego, CA, USA). Compound VCP20 was obtained from Professor Yongqiang Zhu (Nanjing Normal University). Cell
(12)
Pyruvate anaplerosis is a targetable vulnerability in persistent leukaemic stem cells
Nature communications
August 17, 2023
Rattigan KM, Brabcova Z, Sarnello D, Zarou MM, Roy K, Kwan R, de Beauchamp L, Dawson A, Ianniciello A, Khalaf A,
et al
.
CD34 analysis Cells were stained with Annexin V (fluorescein isothiocyanate (FITC,
BioLegend
: Cat#
640906
, 5 µL/test),
7-AAD
(
BD Bioscience
: Cat#
559925
, 5 µL/test) and
CD34
+ (APC,
BD Bioscience
,
clone
581,
(13)
MTH1 protects platelet mitochondria from oxidative damage and regulates platelet function and thrombosis
Nature communications
August 10, 2023
Ding Y, Gui X, Chu X, Sun Y, Zhang S, Tong H, Ju W, Li Y, Sun Z, Xu M,
et al
.
with CRP (5 μg/ml) or thrombin (0.5 U/ml) for 30 min and then stained with
FITC-Annexin-V
(
Biolegend
,
640906
,
1:20
) for 15 min followed by detecting Annexin-V binding by
flow cytometry
. Annexin-V binding was presented
(14)
Nuclear morphology predicts cell survival to cisplatin chemotherapy
Neoplasia
August 1, 2023
Chi-Ju Kim, Anna LK Gonye, Kevin Truskowski, Cheng-Fan Lee, Yoon-Kyoung Cho, Robert H. Austin, Kenneth J. Pienta, Sarah R. Amend
orange
channels.
Flow cytometry analysis
Live cells were incubated with a
FITC-Annexin V
(
BioLegend
, #
640906
) according to the manufacturer's recommendations. Cells were analyzed on an
Attune NxT
Flow Cytometer
(15)
In silico enhancer mining reveals SNS-032 and EHMT2 inhibitors as therapeutic candidates in high-grade serous ovarian cancer
British Journal of Cancer
July 27, 2023
Marcos Quintela, David W. James, Jetzabel Garcia, Kadie Edwards, Lavinia Margarit, Nagindra Das, Kerryn Lutchman-Singh, Amy L. Beynon, Inmaculada Rioja, Rab K. Prinjha,
et al.
bars represent the coefficient of variation. Apoptosis
staining
was performed using Annexin V-FITC (
640906
,
Biolegend
; CA, US) and Propidium iodide (PI,
421301
,
Biolegend
) as per manufacturer’s instructions,
(16)
EHBP1L1, an apicobasal polarity regulator, is critical for nuclear polarization during enucleation of erythroblasts
Blood Advances
July 25, 2023
Ji Wu, Kenta Moriwaki, Tatsuya Asuka, Ritsuko Nakai, Satoshi Kanda, Manabu Taniguchi, Tatsuki Sugiyama, Shin-ichiro Yoshimura, Masataka Kunii, Takashi Nagasawa,
et al.
N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid, 140 mM NaCl, and 2.5 mM CaCl2) and then stained with FITC-conjugated Annexin V (
BioLegend
,
640906
). The fluorescently labeled antibodies are listed in supplemental Table 1. For intracellular
staining
(17)
Metabolic Profiles Point Out Metabolic Pathways Pivotal in Two Glioblastoma (GBM) Cell Lines, U251 and U-87MG
Biomedicines
July 20, 2023
Filipa Martins, David van der Kellen, Luís G. Gonçalves, Jacinta Serpa
Then, cell pellets were incubated with 0.5 µL annexin V-fluorescein isothiocyanate (annexin V-FITC;
640906
,
BioLegend
, San Diego, CA, USA), in
annexin V binding buffer
1× (10 mM
HEPES
(pH 7.4; 391333,
Millipore
,
(18)
Optogenetic Induction of Pyroptosis, Necroptosis, and Apoptosis in Mammalian Cell Lines
BIO-PROTOCOL
July 20, 2023
Kateryna Shkarina, Petr Broz
424001
)Annexin V Pacific Blue (BioLegend, catalog number:
640918
)Annexin V FITC (
BioLegend
, catalog number:
640906
)Annexin V Alexa Fluor 647 (
BioLegend
, catalog number:
640912
)CellEvent
caspase-3
/7 green (
Thermo Fisher Scientific
(19)
A localized hydrogel-mediated chemotherapy causes immunogenic cell death via activation of ceramide-mediated unfolded protein response
Science advances
June 30, 2023
Kar A, Jain D, Kumar S, Rajput K, Pal S, Rana K, Kar R, Jha SK, Medatwal N, Yavvari PS,
et al
.
Allprotect tissue reagent (#
76405
) was purchased from
QIAGEN
, Hilden, Germany. Annexin-FITC
assay
kit (#
640906
) was purchased from
BioLegend
, San Diego, USA. Developer solution (#4908216) and XBT
x-ray
film (#6568307)
(20)
miR-216b-5p promotes late apoptosis/necroptosis in trastuzumab-resistant SK-BR-3 cells
Turkish Journal of Biology
May 23, 2023
İŞTAR BURCU DOLAPÇI, SENEM NOYAN, AYŞEGÜL YÜCEL POLAT, HAKAN GÜRDAL, BALA GÜR DEDEOĞLU
pH: 7.4; 140 mM NaCl, 2.5 mM CaCl2) in a concentration of 1 × 106 cells/mL. Five μL Annexin V-FITC (
640906
,
BioLegend
) and five μL PI (
421301
,
BioLegend
) (0.5 μg/μL) were added and the cells were incubated in
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Reviews
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(1)
1/6/2015
Measurement of Differential Apoptosis of HEK cells in presence of PDGF
Anirban Banerjee
Apoptosis of keratinocytes is a crucial step that balances proliferation to maintain proper epidermal thickness. However keratinocytes from the peripheral area of chronic wounds undergo higher and ...
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FITC Annexin V from BioLegend
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
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