FITC Annexin V 640906 from BioLegend

Product Specs
ItemFITC Annexin V
CompanyBioLegend
Price $194.00Supplier Page View Company Product Page
Catalog Number640906
Conjugate/TagFITC
ApplicationsFC - Quality tested
Size100 tests
Gene Nameannexin A5
NCBI Gene AliasesANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
NCBI Full Gene Nameannexin A5
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
TargetAnnexin V
SpeciesHuman, Mouse, Rat
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) miR-4772-3p serves as a potential diagnostic and prognostic biomarker in septic acute kidney injury by targeting CISD2Ren FailMay 17, 2026Qiang Chen, Gaoshang Zhuapoptosis assay Apoptosis was analyzed via flow cytometry after double staining with Annexin V-FITC (640906, BioLegend, USA) and propidium iodide (PI) (421301, BioLegend, USA). HK-2 cells were harvested, rinsed

(2) Selective Nanobody-Derived Minibodies Targeting Galectin-1 and -7 Reveal Non-Redundant Glyco-Immune Functions and Therapeutic Potential in Triple-Negative Breast CancerJournal of Medicinal ChemistryMay 14, 2026Rita Nehmé, Marlène Fortier, Philippine Granger Joly de Boissel, France Caza, Fanny Fronton, Léa Bourguignon, Tania Charpentier, Camille Fuselier, Sophia Ferchiou, Noémie D’Auteuil, et al.HEPES, 0.14 M NaCl, 2.5 mM CaCl2, pH 7.4) before staining with Annexin V (0.63 μg/mL, Biolegend, cat. #640906) for 15 min at RT, followed by the addition of propidium iodide (0.25 μg/mL, Sigma, cat. #P4170). Apoptosis

(3) Enhancing the Therapeutic Profile of Dapagliflozin: Chitosan Nanoparticle Encapsulation for Intestinal ApplicationsFASEB BioadvMay 14, 2026Agni Klonari, Antrea-Maria Athinodorou, Eirini Papanikolaou, Anastasia Skonta, Myrto G. Bellou, Lampros Lakkas, Konstantinos I. Tsamis, Georgios S. Markopoulos, Haralambos Stamatis, Petros Bozidis, et al.pellet was resuspended in 100 μL Annexin V Binding Buffer (Biolegend 422201). Annexin V (Biolegend 640906) and PI (Biolegend 421301) (5 μg/mL each) were added under dark conditions, and samples were gently

(4) Genetic variation reveals a homeotic long noncoding RNA that modulates human hematopoietic stem cellsCellMay 1, 2026Peng Lyu, Gaurav Agarwal, Chun-Jie Guo, Adam Sychla, Wallace Bourgeois, Tianyi Ye, Chen Weng, Mateusz Antoszewski, Samantha Joubran, Alexis Caulier, et al."SSC (20X), RNase-free",Invitrogen,AM9770 "UltraPure™ SDS Solution, 10%",Invitrogen,15553027 FITC Annexin V,Biolegend,640906 Propidium Iodide,Invitrogen,P1304MP cyclosporin H,Sigma-Aldrich,SML1575 iQ™ SYBR® Green Supermix,Bio-Rad,1708882 MethoCult™ H4434 Classic

(5) miR-99b-5p inhibition drives apoptosis and tumor shrinkage in triple-negative breast cancer: functional characterization through AGO2-RIP-seq and mechanistic insightsFrontiers in OncologyApril 22, 2026Senem Noyan, Kubra Nur Kaplan Ilhan, Muge Ocal Demirtas, Begum Karaoglu Dallı, Bora Ergin, Rabia Sen, Hakan Gurdal, Bala Gur Dedeogluresuspended in 1X Annexin V binding buffer at a concentration of 1 x 106 cells/ml. Annexin V-FITC (5 µl, 640906, BioLegend) and propidium iodide (PI) (5 µl, 0.5 µg/µl, 421301, BioLegend) were then added to the cell

(6) Polycationic dendrimers synergizes with gefitinib to overcome EGFREx19Del-driven resistance in non-small-cell lung cancerDiscover OncologyApril 17, 2026Adriana Cruz, Bruna Abreu, Cindy Mendes, Isabel Lemos, Catarina Freitas-Dias, Rafael Gomes, Bruna Sousa, Filipe Gonçalves, José S. Ramalho, Duarte C. Barral, et al.by centrifugation, 255 × g for 2 min. Cells were stained with 0.5 µL annexin V fluorescein (FITC)-(640906, BioLegend, San Diego, CA, USA), in 1 × annexin V binding buffer (10 mM HEPES—pH 7.4, 150 mM NaCl, 2.5

(7) Doxorubicin exposure leads to cardiac fibroblast dysregulation and worsens fibrotic remodeling in the pathological heart☆J Mol Cell Cardiol PlusApril 13, 2026Sukriti Bagchi, Amy Cai, Alina S. Bilal, Javier Gamez-Luque, Manuel Rosa-Garrido, Bethany Patton, Mrinalini Kala, Christopher C. Glembotski, Erik A. Blackwood1 million cells/mL for subsequent staining. For each 100,000 cells, 10uL Annexin-V (BioLegend, cat# 640906) and 5uL PI (Invitrogen, cat # P3566) was added and incubated for 15 min at room temperature, then analyzed

(8) ATM functions as a rheostat of metabolic stress in small-cell lung cancerbioRxivMarch 19, 2026Debdatta Halder, Utsav Sen, Vrinda Jethalia, Subhamoy Chakraborty, Andrew Elliott, Kedwin Ventura, Ari Vanderwalde, Balazs Halmos, Hossein Borghaei, Tin Htwe Thin, et al.6410914). Cells were then resuspended in 100 μL binding buffer containing 5 μL Annexin V-FITC (BioLegend, 640906) and incubated for 10 min at room temperature in the dark. Propidium iodide (PI) (10 μL) was added,

(9) Enhanced efficacy of a specific HDAC3 inhibitor in combination with 5-azacitidine against diffuse large B-cell lymphoma∗†Blood NeoplasiaMarch 2, 2026Calixte Calaud, Emilio M. Serrano-Lopez, Leonie A. Cluse, Izabela Todorovski, Thomas Mardivirin, Matt R. Teater, Abdelillah Maarad, Noura Tawfic, Peter J. Fraser, Killian Guiraud, et al.was determined by propidium iodide (PI) staining. Apoptosis was assessed by annexin V (catalog no. 640906; BioLegend) and PI double positivity. For CellTrace Violet (CTV) assays, tumor cells were resuspended

(10) Microplastic-Induced Macrophage Dysfunction Drives Lung Tumor Progression through Glutathione ImbalanceACS NanoFebruary 24, 2026Bora Kim, Koung-Min Park, Haerang Lee, Young-Min Hyun(DAPI; Thermo Fisher, D1306) and fluorescein isothiocyanate (FITC)-annexin V antibodies (BioLegend, 640906). Cells positive for both FITC and DAPI were identified as necrotic, whereas FITC-positive cells were

(11) Lipid‐Facilitated Opening of the ADAM10 Sheddase Revealed by Enhanced Sampling SimulationsAdvanced ScienceFebruary 24, 2026Adrien Schahl, Nandan Haloi, Marta Carroni, Shengpan Zhang, Quentin James Sattentau, Erdinc Sezgin, Lucie Delemotte, Rebecca J. Howardbinding buffer (BD Pharmingen 556454) for 20 min at 4°C in the dark with annexin V‐FITC (Biolegend 640906) used at 1:100, near‐IR fixable viability dye (Invitrogen L10119) at 1:1000, fluorophore‐conjugated

(12) Redox-active nitroxides enhance cisplatin efficacy against cervical cancerRedox BiologyFebruary 1, 2026Carl P. Soltau, Debottam Sinha, Lakshita P. Patil, Philip M. Moseley, Cassie L. Rayner, Nigel L. Barnett, Derek J. Richard, Steven E. Bottle, Ian H. Frazer, Alexander P. Martynwith propidium iodide (1:25 at a concentration of 1 mg/mL) and Annexin V FITC (1:50) (BioLegend, Cat#640906) in Annexin V binding buffer (BioLegend, Cat#422201) at 4 °C for 30mins. For the γH2AX measurement assay,

(13) Remodelling of the bone marrow vasculature induced by venetoclax and azacitidine damageBlood JournalJanuary 29, 2026Steven Ngo, Giuseppe Alessandro D'Agostino, Despoina Papazoglou, Fatihah Mohamad Mohamad Nor, Katja Finsterbusch, Khadidja Habel, Alessandra Ferrelli, Fernando Anjos-Afonso, Dominique Bonnetinitially immunostained with the markers mentioned above, then stained with 5 μl of annexin-V reagent (640906, Biolegend) for 15 minutes at room temperature. Propidium Iodide (51-66211E, BD Biosciences) was used

(14) Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatinFrontiers in ImmunologyJanuary 21, 2026Mei Jia, Dexian Kong, Jiaxu Fan, Bingqian Heng, Jian Zhao, Binghui Li, Shuang Liu, Peng Kong, Yabin Liutransferred to a 5 mL flow cytometry tube, and 5 μL of fluorescently labeled Annexin V (BioLegend, cat. no. 640906) and 7-AAD viability dye (BioLegend, cat. no. 420403) were added for staining. The cells were gently

(15) Multimodal profiling of pancreatic cancer reveals a TIMP-1-dominated secretory profile determining pro-tumor immunoinstruction in human cancersCell Reports MedicineJanuary 20, 2026Julian Frädrich, Carmen Mota Reyes, Michel Hendel, Vanessa Brunner, Batu Toledo, Damjan Manevski, Alexander Sommer, Daniel Häußler, Dominik Beck, Daniele Lucarelli, et al.LipidTOX™ Red Neutral Lipid Stain,ThermoFisher Scientific,CAT# H34476 Annexin V FITC,BioLegend,CAT# 640906 eBioscience 7-AAD viability dye,ThermoFisher Scientific,CAT# 00-6993-50 ,, Critical commercial assays,Critical

(16) Sequence-dependent splicing dysregulation drives therapy resistance in pediatric AMLCell Reports MedicineJanuary 20, 2026Yue Huang, Peifang Xiao, Lei Qin, Li Gao, Yang Zhao, Jingru Sui, Wenting Hu, Lei Zhou, Nan Han, Xuan Lv, et al.RRID:AB_2142033 Rabbit IgG HRP linked antibody,Cytiva,Cat#NA934; RRID:AB_772206 FITC Annexin V,Biolegend,Cat#640906; RRID:AB_2561292 ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, Pyronic/pcDNA3.1(−),addgene,Cat#51308;

(17) Photothermal Therapy-Induced Immunogenic Cell Death Synergistically Enhances the Therapeutic Effect of Immune Checkpoint InhibitorsCancers (Basel)January 16, 2026Shogo Yasuda, Yui Horikawa, Mei Ohashi, Mai Amou, Taisei Kanamori, Duan Runjing, Yuta Tamemoto, Wei Xu, Takuro Niidome, Akihiro Hisaka, et al.(Thermo Fisher Scientific, Waltham, MA, USA, #62248) and AnnexinV-FITC (BioLegend, San Diego, CA, USA, #640906) to detect apoptotic and necrotic cell populations, respectively. The excitation and emission wavelengths

(18) Methionine Restriction Reduces Lung Cancer Progression and Increases Chemotherapy ResponsebioRxivJanuary 16, 2026Kassandra J. Naughton, Xiulong Song, Avery R. Childress, Erika M. Skaggs, Aria L. Byrd, Christian M. Gosser, Dave-Preston Esoe, Tanner J. DuCote, Daniel R. Plaugher, Alexsandr Lukyanchuk, et al.double positive stain, 7AAD single stain, and unstained sample. 5 μL of FITC antibody (BioLegend, CAT#: 640906) and 5 μL of 7AAD (Invitrogen, CAT#: A1310) were added to each sample and to the double positive tube.

(19) Mutations in VPS18 lead to a neutrophil maturation defect associated with disturbed vesicle homeostasisCell Death & DiseaseJanuary 12, 2026Jincheng Gao, Almke Bader, Monika I. Linder, Jingyuan Cheng, Mathis Richter, Raul da Costa, Annette Zehrer, Karl Mitt, Bastian Popper, Felix Meissner, et al.(Invitrogen, #S34859). For cell death analysis, cells were stained using FITC-Annexin V (Biolegend, #640906), SYTOX Red dead cell stain and tetramethylrhodamine methyl ester (TMRM, Invitrogen, #T668). If not

(20) Damage-induced pyroptosis drives endogenous thymic regeneration by activating the purinergic receptor P2Y2Cell Death & DiseaseJanuary 3, 2026Sinéad Kinsella, Cindy A. Evandy, Kirsten Cooper, Erin Kirsche, Makya Warren, Paul deRoos, Antonella Cardinale, Lorenzo Iovino, David Granadier, Colton W. Smith, et al.(Cell Signaling, 9669S). Apoptosis and pyroptosis were assessed by adding Annexin V-FITC (Biolegend, 640906), Annexin V binding buffer (BioLegend, 422201,) and Propidium Iodide (Invitrogen, BMS500PI). Gasdermin

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1/6/2015Measurement of Differential Apoptosis of HEK cells in presence of PDGF Anirban BanerjeeApoptosis of keratinocytes is a crucial step that balances proliferation to maintain proper epidermal thickness. However keratinocytes from the peripheral area of chronic wounds undergo higher and ... Read Review

FITC Annexin V from BioLegend

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FITC Annexin V from BioLegend

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