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FITC Annexin V
FITC Annexin V from BioLegend
(1)
Write a review
Citations:
(270)
Supplier Page
Supplier Page from
BioLegend for
FITC Annexin V
Description
FITC Annexin V
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
FITC Annexin V
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
640906
Conjugate/Tag
FITC
Applications
Flow Cytometry (FC)
Quantity
100 tests
Gene Name
annexin A5
NCBI Gene Aliases
ANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
NCBI Full Gene Name
annexin A5
Format
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Target
Annexin V
Species
Human, Mouse, Rat
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (270)
(21)
Bromodomain inhibitor i-BET858 triggers a unique transcriptional response coupled to enhanced DNA damage, cell cycle arrest and apoptosis in high-grade ovarian carcinoma cells
Clinical Epigenetics
April 15, 2023
Marcos Quintela, David W. James, Agne Pociute, Lydia Powell, Kadie Edwards, Zoe Coombes, Jetzabel Garcia, Neil Garton, Nagindra Das, Kerryn Lutchman-Singh,
et al.
graphed using GraphPad Prism (V9). Apoptosis
staining
was performed using Annexin V-FITC (
BioLegend
,
640906
) and propidium iodide (PI,
BioLegend
,
421301
) as per manufacturer’s instructions, which included the
(22)
A high throughput bispecific antibody discovery pipeline
Communications Biology
April 7, 2023
Aude I. Segaliny, Jayapriya Jayaraman, Xiaoming Chen, Jonathan Chong, Ryan Luxon, Audrey Fung, Qiwei Fu, Xianzhi Jiang, Rodrigo Rivera, Xiaoya Ma,
et al.
of the cells were collected and stained with 7AAD (#50-850-582, Fisher Scientific) and Annexin V (#
640906
,
Biolegend
), and PE/Cyanine 7 anti-human CD4 antibody (#
317414
,
Biolegend
), APC/Cyanine 7 anti-human
(23)
Caspase-8 is required for HSV-1-induced apoptosis and promotes effective viral particle release via autophagy inhibition
Cell death and differentiation
April 1, 2023
Marino-Merlo F, Klett A, Papaianni E, Drago SFA, Macchi B, Rincón MG, Andreola F, Serafino A, Grelli S, Mastino A,
et al
.
Sigma-Aldrich
(Taufkirchen, Germany).
FITC-Annexin-V
was bought from
BioLegend
(San Diego, CA, USA, #
640906
) and the p38 MAPK inhibitor SB203580 from Calbiochem (San Diego, CA, USA). Recombinant CD95/FasL was
(24)
Targeting Tn-positive tumors with an afucosylated recombinant anti-Tn IgG
Scientific Reports
March 28, 2023
Yasuyuki Matsumoto, Nan Jia, Jamie Heimburg-Molinaro, Richard D. Cummings
°C in CO2 incubator. Cells were resuspended and co-stained with FITC-labeled Annexin V antibody (Cat#
640906
,
BioLegend
, diluted at
1:20
) and 1 μg/mL of propidium iodide (PI) (Cat#
P3566
,
Invitrogen
) in Annexin
(25)
PFKFB2-mediated glycolysis promotes lactate-driven continual efferocytosis by macrophages
Nature metabolism
March 1, 2023
Schilperoort M, Ngai D, Katerelos M, Power DA, Tabas I.
and resuspended in
annexin V Binding Buffer
containing
1:20
FITC Annexin V
solution (
Biolegend
, no.
640906
). After 15 minutes, the cells were analyzed using the above-mentioned
flow cytometer
. Data analysis
(26)
Characterization of proteogenomic signatures of differentiation of CD4+ T cell subsets
DNA research : an international journal for rapid publication of reports on genes and genomes
February 1, 2023
Kanno T, Konno R, Miyako K, Nakajima T, Yokoyama S, Sasamoto S, Asou HK, Ohzeki J, Kawashima Y, Hasegawa Y,
et al
.
Preparation. After 2 days
cell culture
, cells were collected and stained with anti-Annexin V FITC (
BioLegend
#
640906
) for 20 min on ice and then incubated with
anti-FITC MicroBeads
(
Miltenyi Biotec
#
130-048-701
) for 20
(27)
Combined inhibition of aurora kinases and Bcl-xL induces apoptosis through select BH3-only proteins
Journal of Biological Chemistry
February 1, 2023
Jian Li, Cheng-Hsun Chen, Katelyn L. O’Neill, Valerie J. Fousek-Schuller, Adrian R. Black, Jennifer D. Black, Jingjing Zhang, Xu Luo
Thermo Fisher Scientific
(catalog no.:
22330
). Annexin V-FITC was purchased from
BioLegend
(catalog no.:
640906
). Propidium iodide (PI) solution was purchased from G-Biosciences (catalog no.: 786-1272). Antibodies
(28)
Molecular DNA dendron vaccines
Proceedings of the National Academy of Sciences
January 31, 2023
Max E. Distler, John P. Cavaliere, Michelle H. Teplensky, Michael Evangelopoulos, Chad A. Mirkin
Annexin V binding buffer
(
BioLegend
) containing 0.5 μL each of 7-AAD (Fisher, 50169259) and Annexin V (
BioLegend
,
640906
). This
staining
solution was left with cells for 15 min at room temperature in the dark prior to
flow cytometry
(29)
Damage-induced pyroptosis drives endogenous thymic regeneration via induction of Foxn1 by purinergic receptor activation
bioRxiv
January 25, 2023
Sinéad Kinsella, Cindy A. Evandy, Kirsten Cooper, Antonella Cardinale, Lorenzo Iovino, Paul deRoos, Kayla S. Hopwo, Colton W. Smith, David Granadier, Lucas B. Sullivan,
et al.
(
Cell signaling
,
9669S
). Apoptosis and pyroptosis was assessed by adding Annexin V-FITC (
Biolegend
,
640906
),
Annexin V binding buffer
(
BioLegend
,
422201
) and
Propidium Iodide
(
Invitrogen
,
BMS500PI
). Gasdermin
(30)
The poly(C)-binding protein Pcbp2 is essential for CD4+ T cell activation and proliferation
iScience
January 20, 2023
Massimo Martinelli, Gabrielle Aguilar, David S.M. Lee, Andrew Kromer, Nhu Nguyen, Benjamin J. Wilkins, Tatiana Akimova, Ulf H. Beier, Louis R. Ghanem
Flow cytometric
detection of apoptosis and
necrosis
was performed with FITC–labeled Annexin V (cat. #
640906
,
BioLegend
) and the live/dead fixable aqua dead
cell stain
kit (cat. #
L34957
;
Thermofisher Scientific
).
T cell
(31)
Large‐scale genetic screens identify BET ‐1 as a cytoskeleton regulator promoting actin function and life span
Aging Cell
January 1, 2023
Gilberto Garcia, Raz Bar‐Ziv, Maxim Averbukh, Nirmalya Dasgupta, Naibedya Dutta, Hanlin Zhang, Wudi Fan, Darius Moaddeli, C. Kimberly Tsui, Toni Castro Torres,
et al.
treatment with 30 mM H2O2 was used as a positive control for cell death. Annexin V FITC (
Biolegend
,
640906
) and live/dead Aqua (
ThermoFisher
,
L34966
)
staining
was performed as per manufacturer's protocol. Briefly,
(32)
Use of phage display biopanning as a tool to design CAR-T cells against glioma stem cells
Frontiers in oncology
January 1, 2023
Potez M, Snedal S, She C, Kim J, Thorner K, Tran TH, Ramello MC, Abate-Daga D, Liu JKC.
minutes at 4°C in the dark. Cells were then washed, secondarily stained with Annexin-V-FITC (
Biolegend
,
640906
) and
DAPI
, incubated for 15 minutes at room temperature in the dark, and then analyzed. Data acquisition
(33)
Impaired CXCL12 signaling contributes to resistance of pancreatic cancer subpopulations to T cell-mediated cytotoxicity
OncoImmunology
December 31, 2022
Yuan-Na Lin, Marcel O. Schmidt, Ghada M. Sharif, Eveline E. Vietsch, Amber J. Kiliti, Megan E. Barefoot, Anna T. Riegel, Anton Wellstein
(34)
NUC-7738 regulates β-catenin signalling resulting in reduced proliferation and self-renewal of AML cells
PLOS ONE
December 15, 2022
Akbar Muhammed Shahid, In Hwa Um, Mustafa Elshani, Ying Zhang, David James Harrison
of
Annexin V Binding Buffer
(
422201
,
BioLegend
) and 5 μL of Annexin-V conjugated to FITC (
BioLegend
,
640906
). Cells were then vortexed and incubated at room temperature away from light for 15 minutes. An additional
(35)
The hepatic integrated stress response suppresses the somatotroph axis to control liver damage in nonalcoholic fatty liver disease
Cell Reports
December 13, 2022
Rika Ohkubo, Wei-Chieh Mu, Chih-Ling Wang, Zehan Song, Marine Barthez, Yifei Wang, Nathaniel Mitchener, Rasul Abdullayev, Yeong Rim Lee, Yuze Ma,
et al.
,
Thermo Fisher Scientific
,Cat#
62247
Propidium iodide solution
,
Biolegend
,Cat#
421301
FITC Annexin V
,
BioLegend
,Cat#
640906
Formaldehyde,Thermo Fisher Scientific,Cat# F79-500 ,, Critical commercial
assays
,Critical commercial
(36)
Precursor‐Directed Synthesis of Apoptosis‐Initiating N ‐Hydroxyalkyl Phenylbenzoisoquinolindione Alkaloids
ChemistryOpen
December 7, 2022
Yu Chen, Hans‐Martin Dahse, Christian Paetz, Bernd Schneider
apoptotic cells by
flow cytometry
, the described protocols for Annexin V FITC (
Biolegend
, cat. no.
640906
) and Fixable Viability Dye eFluor780 (
eBioscience
, cat. no.
65–0865‐14
) were used. Supporting Information
(37)
STING agonism reprograms tumor-associated macrophages and overcomes resistance to PARP inhibition in BRCA1-deficient models of breast cancer
Nature Communications
December 1, 2022
Qiwei Wang et al.
treatment) were washed twice with cold PBS, and then incubated with 5 μL
FITC Annexin V
(
BioLegend
, #
640906
) and 10 μL 7-AAD (
BioLegend
, #
420404
) in 100 μL
Annexin V binding buffer
(
BioLegend
, #
422201
) at room
(38)
MEK and MCL-1 sequential inhibition synergize to enhance rhabdomyosarcoma treatment
Cell Death Discovery
December 1, 2022
Clara Alcon et al.
h of
incubation
with the specified treatments, cells were analyzed using Annexin V (FITC Annexin V,
640906
,
BioLegend
) and propidium iodide (PI) (1056, BioVision, Milpitas, California, USA) and analyzed on a
(39)
The 3D enhancer network of the developing T cell genome is shaped by SATB1
Nature communications
November 14, 2022
Zelenka T, Klonizakis A, Tsoukatou D, Papamatheakis DA, Franzenburg S, Tzerpos P, Tzonevrakis IR, Papadogkonas G, Kapsetaki M, Nikolaou C,
et al
.
were anti-DNA PI-conjugated (Biolegend, 79997), anti-Phosphatidylserine FITC conjugated (
Biolegend
,
640906
, 1/100), anti-CD4 PE-conjugated (
Pharmingen
,
553730
, 1/100), anti-
CD8a
APC conjugated (
Biolegend
,
100712
,
(40)
Expansion and differentiation of ex vivo cultured erythroblasts in scalable stirred bioreactors
Biotechnology and Bioengineering
November 1, 2022
Joan Sebastián Gallego‐Murillo, Giulia Iacono, Luuk A. M. van der Wielen, Emile van den Akker, Marieke von Lindern, Sebastian Aljoscha Wahl
(live/dead stain;
1:2000
dilution;
Invitrogen
cat#
P3566
); (v) AnnexinV‐FITC (
1:1000
dilution;
BioLegend
cat#
640906
), DRAQ7 (live/dead stain;
1:200
dilution; Thermo Fischer Scientific cat#D15106); (vi) DRAQ5 (
nuclear stain
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Reviews
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1/6/2015
Measurement of Differential Apoptosis of HEK cells in presence of PDGF
Anirban Banerjee
Apoptosis of keratinocytes is a crucial step that balances proliferation to maintain proper epidermal thickness. However keratinocytes from the peripheral area of chronic wounds undergo higher and ...
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FITC Annexin V from BioLegend
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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