Human IL-4, premium grade 130-093-920 from Miltenyi Biotec

(1) Respiratory syncytial virus inhibits type I interferon signaling to maintain HLA-DM expression in CD1c+ dendritic cellsiScienceAugust 21, 2026Weiyee Ong, Richard Anthony Hopkins, Enjun Yang, Novita, Najwa Talib, Bijin Au, John Edward Connollyconcentration of cytokines 1000 IU/mL GM-CSF (Miltenyi Biotec, 130-093-868), 500 IU/mL IL-4 (Miltenyi Biotec, 130-093-924) and 50 ng/mL Flt3-L (Miltenyi Biotec, 130-096-480) was added and incubated for 24 h at 37°C. At day

(2) Immunogenomic Profiling Reveals Targets for Gene Therapy in Pediatric Brain TumorsNeuro-Oncology AdvancesJuly 27, 2026Giulia Rovesti, Alejandro Fernandez Woodbridge, Haidong Yao, Alessia Pancaldi, Chiara Chiavelli, Francesca Gatto, Ulrika Sandvick, Matti Sällberg, Jonas Fuxe, Massimo Dominici, et al.supplemented with GM-CSF (800 UI/ml, Miltenyi Biotec, 130-093-867), IL-4 (200 U/ml, Miltenyi Biotec, 130-093-922), AB serum (10%, SigmaAldrich, H3667), for 5 days before cryopreservation. In Vitro Stimulation Assay Neoantigen-reactive

(3) In vitro recapitulation of intramuscular mRNA vaccination with naive and recall antigens using a human lymphoid follicle chip platformiScienceJuly 17, 2026Yunhao Zhai, Min Wen Ku, Ka Yang, Aditya Patil, Pranav Prabhala, Liqun He, Yuncheng Man, Mizanur Rahman, Kavya Menon, Sven B. Spörri, et al.Coulter,Cat#C36628 Human Interleukin-2 (IL-2),Miltenyi Biotec,130-097-742 Human Interleukin-4 (IL-4),Miltenyi Biotec,130-093-922 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, EasySep™ Human B Cell Enrichment Kit

(4) Glycosaminoglycan-functionalized hydrogels for sustained delivery of tissue inhibitor of metalloproteinase-3 mediating matrix metalloprotease inhibition and extracellular matrix stabilizationBioactive MaterialsJuly 1, 2026Fabian Junker, Stefan Rupf, Paula Marie Schindler, Cedric Wilden, Mathias Hohl, Gloria Ruiz-Gómez, M. Teresa Pisabarro, Selina Wrublewsky, Caroline Bickelmann, Charlotte Berhorst, et al.lipopolysaccharide (LPS) (InvivoGen #tlrl-3pelps) or containing 20 ng/mL interleukin-4 (IL-4) (Miltenyi, # 130-093-920) IL-13 (Miltenyi, #130-112-410) for polarization into M2. Where indicated, the final polarization medium

(5) Ex vivo-generated conventional dendritic cells type 1 and type 2 from blood progenitors induce potent antigen-specific T-cell immunityOncoimmunologyJune 30, 2026Jorge Cuenca-Escalona, Kevin Bos, Johanna Bödder, Tom van Oorschot, Tjitske Duiveman-de Boer, Georgina Flórez-Grau, I. Jolanda M. de Vries(130-093-867, Miltenyi Biotec), 1000 U/ml IFN-a2 (130-093-874, Miltenyi Biotec), and 100 U/ml IL-4 (130-093-924, Miltenyi Biotec). On day 14, cultures were supplemented with fresh media (25% of the volume used on

(6) Combining PD-L1 blockade with a second-generation HSP110 inhibitor enhances antitumor immunity in colorectal cancerCell Death & DiseaseMay 19, 2026María Jimena Abrey-Recalde, Flavie Mialhe, Thi Khanh Le, Abdelmnim Radoua, Lisa Lagorgette, Rim Belkaid, Adiilah Mamode Cassim, Daniel Gonzalez, Elise Jacquin, Jessica Gobbo, et al.Miltenyi Biotec) and 100 ng/mL LPS (#L2887, Sigma-Aldrich) for an M1-like phenotype or 20 ng/mL of IL-4 (#130-093-922, Miltenyi Biotec) for an M2-like phenotype during 48 h. When indicated, the HSP110 inhibitors C33 or

(7) Overcoming claudin family homology: discovery of ARC101, a highly potent CLDN6-specific T-cell engager with a novel CD3 binder for ovarian adenocarcinomaMAbsMarch 10, 2026Danlin Yang, Neha Kamran, Gururaj Shivange, Kavita Kumari, Prabhu Srinivas Yavvari, Viduth K. Chaugule, Vishal Mahajan, Bridget Larkin, Scott R. Brodeur, Joe Erhardt, et al.with 1000 IU/mL GM-CSF (Miltenyi Biotec, Cat# 130–093-866), 1000 IU/mL IL-4 (Miltenyi Biotec, Cat# 130–093-922), and 50 µg/mL gentamicin (Thermo-Fisher Scientific, Cat# 15710064). Cells were cultured in 24-well

(8) IGF2BP2 Deficiency in Macrophages Impairs Migration, Reprograms Metabolism, and Limits Tumor ProgressionInternational Journal of Biological SciencesFebruary 18, 2026Hanna S. Schymik, Selina Wrublewsky, Marcus Höring, Gerhard Liebisch, Simon Both, Gilles Gasparoni, Caroline Bickelmann, Hanah Robertson, Charlotte Dahlem, Jörn Walter, et al.#tlrl-peklps, Invivogen, Toulouse, France) for M1 polarization, either 20 ng/ml interleukin-4 (IL-4) (#130-093-921, Miltenyi Biotech, Bergisch Gladbach, Germany) for M2 polarization, or left without further supplementation

(9) What Is the Role of Giant Endosomal Sorting Complexes Required for Transport (ESCRT) Structures in T Cell Activation?Advanced BiologyJanuary 1, 2026Anthi Psoma, Femmy C. Stempels, Rinse de Boer, Geert van den BogaartThermo Fisher Scientific), 1% antibiotic antimycotic (AA; 15240062; Gibco), 300 U/ml interleukin‐4 (130‐093‐924; Miltenyi), and 450 U/ml granulocyte macrophage colony‐stimulating factor (130‐093‐867; Miltenyi) for

(10) The chaperone GRP94 interacts with the proprotein convertase furin and regulates TGF-beta maturation in human primary M2 macrophagesCell Death DiscovDecember 15, 2025Valentin Baverel, Fangmin Wang, Carmen Garrido, Evelyne Kohlidifferentiation medium renewal after 3 days. Macrophages were then activated with 20 ng/mL interleukine-4 (IL-4, 130-093-922, Miltenyi) in serum free Opti-MEM medium for 24 h. The GRP94 inhibitor PU-WS13 (HY-18680, MedChemExpress,

(11) Immunopeptidome analysis reveals SERPINB3 as an autoantigen driving eczematized psoriasisSci AdvOctober 24, 2025Manja Jargosch, Jomy Kuruvila, Emanuele Scala, Johannes Grosch, Jessica Eigemann, Sophia Wasserer, Shalva Lekiashvili, Nico Trautwein, Daniel Johannes Kowalewski, Alexander Böhner, et al.colony-stimulating factor (GM-CSF; Miltenyi Biotec, 130-093-864, 150 U/ml) and IL-4 (Miltenyi Biotec, 130-093-920, 150 U/ml) as described previously (71). Before coculture with lesional T cells, differentiated MODCs

(12) Immunogenicity of single-chain antibodies: germlining of a VHH lowers T-cell activation from epitopes in FR2 and CDR regionsMAbsOctober 13, 2025Remi Giraudet, Adrien Laroche, Benjamin Chalopin, Steven Dubois, Evelyne Correia, Isabelle Staropoli, Olivier Schwartz, Bernard Maillère, Hervé Nozachd of culture in AIM-V medium (Invitrogen) supplemented with 1,000 U/mL recombinant human (rh) IL-4 (130–093-924, Miltenyi Biotec) and rhGM-CSF (130–093-867, Miltenyi Biotec). Immature DCs (20,000 per well) were loaded

(13) Phosphorylation of VAMP3 couples IL-6 exocytosis to dendritic cell activationJournal of Cell ScienceOctober 1, 2025Tongxiang Chen, Anthi Psoma, Shweta Mahajan, Martin ter Beest, Peter Linders, Giulia Franciosa, Frans Bianchi, Geert van den van den Bogaart, Harry Warnerdifferentiating human peripheral blood CD14-positive monocytes with interleukin-4 (300 µg/ml; Miltenyi, 130-093-924) and granulocyte-macrophage colony-stimulating factor (GM-CSF; 450 µg/ml; Miltenyi ,130-093-867) for

(14) ESPEC-SUIT: a versatile and robust platform to identify and track antigen-specific T cell receptors in patients with cancerJournal for ImmunoTherapy of CancerSeptember 21, 2025Katharina Lindner, Saskia R Stange, Julian Benedikt Hlawatsch, Moritz Hamberger, Tamara Boschert, Chin Leng Tan, Ingrid Hülsmeyer, Samira Moreen Sachse, Simone Jünger, Alexander Ernst, et al.monocytes (130-050-201, Miltenyi) differentiated for 5 days in X-Vivo20 containing 500 IU/mL interleukin-4 (130-093-921, Miltenyi) and 560 IU/mL granulocyte macrophage colony-stimulating factor (300-03-100, PeproTech)).

(15) hafoe: an interactive tool for the analysis of chimeric AAV libraries after random mutagenesisGene TherapyJuly 8, 2025Tatevik Jalatyan, Erik Aznauryan, Rokib Hasan, Valeri Vardanyan, Stepan Nersisyan, David B. Thompson, Noah Davidsohn, Sanya Thomas, Simon van Haren, Jenny Tam, et al.Monocytes were cultured in Cellgro DC medium (Cellgenix GMBH, 20801-0500) with 250 IU/mL IL-4 ((Miltenyi, 130-093-922), and 800 IU/mL GM-CSF (Miltenyi, 130-093-866), and incubated in a 6-well plate at 37 °C and 5% CO2

(16) Inflammation Promotes Proteolytic Processing of the Prohormone Chromogranin A by MacrophagesJ Endocr SocMay 15, 2025Melina Ioannidis, Hendrik van Dijk, Elke M Muntjewerff, Venkat R Chirasani, Harry Warner, Willemijn van den Dool, Pieter Grijpstra, Frans Bianchi, Sushil K Mahata, Geert van den Bogaart(Sigma-Aldrich, L2630) and 20 ng/mL interferon-γ (IFN-γ) (Peprotech, 300-02) or 300 U/mL IL-4 (Miltenyi Biotec, 130-093-924) for 48 hours toward a proinflammatory- (M1) or anti-inflammatory (M2) phenotype, respectively. Neutrophil

(17) Glucose deprivation and identification of TXNIP as an immunometabolic modulator of T cell activation in cancerFront ImmunolApril 7, 2025Agathe Dubuisson, Adèle Mangelinck, Samantha Knockaert, Adrien Zichi, Etienne Becht, Wendy Philippon, Sandra Dromaint-Catesson, Manon Fasquel, Fabien Melchiore, Nicolas Provost, et al.P/S (cat no. 15140, Gibco), HEPES 10 mM (cat no. 15630, Gibco), 50 ng/mL interleukin-4 (IL-4, cat no. 130-093-922, Miltenyi) and 100 ng/mL GM-CSF (cat no. 130-093-866, Miltenyi) (25). On day 4, 20% of MoDC media was

(18) Endogenous CD4+ T Cells That Recognize ALK and the NPM1::ALK Fusion Protein Can Be Expanded from Human Peripheral BloodCancer Immunology ResearchApril 2, 2025Serena Stadler, Rafael B. Blasco, Vijay Kumar Singh, Christine Damm-Welk, Amin Ben-Hamza, Carlotta Welters, Leo Hansmann, Roberto Chiarle, Wilhelm Woessmann1.5–3×106 cells per well in X-Vivo 15 (Lonza, #02–053Q) supplemented with 500 U/ml IL-4 (Miltenyi Biotec, #130-093-922) and 1,000 U/ml GM-CSF (PeproTech, #300–03). After 30h, cells were matured by the addition of 10 ng/ml

(19) Endogenous CD4+ T cells that recognize ALK and the NPM1::ALK fusion protein can be expanded from human peripheral bloodCancer Immunol ResApril 2, 2025Serena Stadler, Rafael B. Blasco, Vijay Kumar Singh, Christine Damm-Welk, Amin Ben-Hamza, Carlotta Welters, Leo Hansmann, Roberto Chiarle, Wilhelm Woessmann1.5–3×106 cells per well in X-Vivo 15 (Lonza, #02–053Q) supplemented with 500 U/ml IL-4 (Miltenyi Biotec, #130-093-922) and 1,000 U/ml GM-CSF (PeproTech, #300–03). After 30h, cells were matured by the addition of 10 ng/ml

(20) An essential role for TASL in mouse autoimmune pathogenesis and Toll-like receptor signalingNature communicationsJanuary 24, 2025Lau L, Cariaga TA, Chang AB, Lane JH, Purtha WE, Rapaport AS, Hu R, Konno H, Bulloch DN, Rardin MJ, et al.containing 1x Pen/Strep (Invitrogen), 5% human AB serum (Sigma;H3667), 125 IU/mL of human IL-4 (Miltenyi; 130-093-922), and 8 U/ml of multimeric CD40 ligand (Miltenyi; 130-098-775). Media was changed every 3–4 days. For

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Human IL-4, premium grade from Miltenyi Biotec

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Human IL-4, premium grade from Miltenyi Biotec

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