PsbA - D1 protein of PSII positive control/quantitation standard AS01 016S from Agrisera

Product Specs
ItemPsbA - D1 protein of PSII positive control/quantitation standard
CompanyAgrisera
Price EUR185.00Supplier Page View Company Product Page
Catalog NumberAS01 016S
StorageStore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
FormatLyophilized in glycerol
Typeprotein
Research AreaPlant Biology, Photosynthesis
Molecular WeightThe standard has an actual MW of 41.5 kDa, The presence of a His6 tag causes it to run ~1.7 kDa higher on the gel than the native protein, Note that in most systems, PsbA migrates with an apparent MW of between 30 and 35 kDa
ApplicationsWestern blot
Size100 µl

Specifications/Features

Standard curve: 3 loads are recommended (0.5, 2 and 4Î¼l).For most applications a sample load of 0.2Î¼g of chlorophyll will give a PsbA signal in this range.Positive control:a 2Î¼l load per well is optimal for most chemiluminescent detection systems.Non-disulphie dependent dimers and complexes can be also detected using standard western blot methods with more sensitive detection reagents as ECL Advance or West Pico when loading per well more standard than recommended. They have not been included in the standard calibration.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing

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Agrisera

Box 57

Vännäs

Sweden

Phone: +46 (0) 935 33033

Email: info@agrisera.com
orders@agrisera.com

Company Profile

Website: http://www.agrisera.com

(1) A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in ArabidopsiseLifeFebruary 25, 2021Rosa Pipitone, Simona Eicke, Barbara Pfister, Gaetan Glauser, Denis Falconet, Clarisse Uwizeye, Thibaut Pralon, Samuel C Zeeman, Felix Kessler, Emilie Demarsy

(2) Effects of Temperature and Nutrient Supply on Resource Allocation, Photosynthetic Strategy, and Metabolic Rates of Synechococcus spJournal of phycologyJune 1, 2020Fernández-González C, Pérez-Lorenzo M, Pratt N, Moore CM, Bibby TS, Marañón E.

(3) Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutumProceedings of the National Academy of SciencesAugust 27, 2019Orly Levitan et al.was used as the secondary antibody. Positive control protein standards (Agrisera) used include PsbA (AS01 016S), PsaC (AS04 042S), and AtpB (AS03 030S). The blots were developed with Amersham ECL Select Detection

(4) Quantitative insights into the cyanobacterial cell economyeLifeFebruary 4, 2019Zavřel T, Faizi M, Loureiro C, Poschmann G, Stühler K, Sinetova M, Zorina A, Steuer R, Červený J.(1:1000) "Peptide, recombinant protein",Recombinant PsbA from Synechocystissp. PCC 6803,Agrisera,Cat. #: AS01 016S,41.5 kDa "Peptide, recombinant protein",Recombinant PsaC from Synechocystissp. PCC 6803,Agrisera,Cat.

(5) Combined effects of ocean acidification and warming on physiological response of the diatom Thalassiosira pseudonana to light challengesMarine environmental researchApril 1, 2018Yuan W, Gao G, Shi Q, Xu Z, Wu H.

(6) Interactive effects of nitrogen and light on growth rates and RUBISCO content of small and large centric diatomsPhotosynthesis ResearchJanuary 1, 2017Gang Li, Douglas A. Campbellrange of 125, 62.5, 31.25 and 15.625 fmol PsbA standard per standard lane (Agrisera, www.agrisera.se, AS01 016S). Electrophoresis was run for 25 min at 200 V and the proteins were transferred to a polyvinylidene

(7) A Hard Day's Night: Diatoms Continue Recycling Photosystem II in the DarkFrontiers in Marine ScienceNovember 8, 2016Gang Li, Amy D. Woroch, Natalie A. Donaher, Amanda M. Cockshutt, Douglas A. Campbellloaded in parallel with a range of PsbA, PsbD, PsaC or FtsH protein standards (Agrisera, www.agrisera.se, AS01 016S; AS09 146S; AS04 042S; AS11 1789S) to establish a standard curve for quantitation of the target protein.

(8) Changes in the Rubisco to photosystem ratio dominates photoacclimation across phytoplankton taxaPhotosynthesis researchJune 1, 2015Jennifer Marie-Rose Vandenhecke, Jake Bastedo, Amanda M Cockshutt, Douglas A Campbell, Yannick Huot

(9) The nitrogen costs of photosynthesis in a diatom under current and future pCO2The New phytologistJanuary 1, 2015Gang Li, Christopher M Brown, Jennifer A Jeans, Natalie A Donaher, Avery McCarthy, Douglas A Campbell

(10) Large centric diatoms allocate more cellular nitrogen to photosynthesis to counter slower RUBISCO turnover ratesFrontiers in Marine ScienceDecember 9, 2014Yaping Wu, Jennifer Jeans, David J. Suggett, Zoe V. Finkel, Douglas A. Campbellprotein quantitation standard curves for each specific antibody (AgriSera, AS03-037, and AS01-017S; AS05-084 and AS01-016S; AS08 330 and AS08 330S, AS10 939 and AS04 042S). The secondary antibody goat anti-rabbit IgG was used for

(11) Effects of Light, Food Availability and Temperature Stress on the Function of Photosystem II and Photosystem I of Coral SymbiontsPLoS ONEJanuary 13, 2012Mia O. Hoogenboom, Douglas A. Campbell, Eric Beraud, Katrina DeZeeuw, Christine Ferrier-Pagèscurves created using protein standards run in parallel with sample protein extracts; PsbA (AgriSera AS01 016S), PsaC (AS04 042S), and RbcL (AS01 017S). Data analysis Analysis of variance (ANOVA) was used to quantify

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PsbA : D1 positive control/quantitation standard

PsbA - D1 protein of PSII positive control/quantitation standard from Agrisera

Description

Specifications/Features

Agrisera

Citations (11)

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PsbA - D1 protein of PSII positive control/quantitation standard from Agrisera

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