Histone H10Human, Recombinant M2501S from New England Biolabs

Product Specs
ItemHistone H10Human, Recombinant
CompanyNew England Biolabs
Price $95.00 Inquire
Catalog NumberM2501S
Molecule NameHistone H1
Source/Expression SystemRecombinant
Size100 µg
ApplicationsFunctional Genomics
TypeProteins
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Material properties of biomolecular condensates emerge from nanoscale dynamicsProc Natl Acad Sci U S AJune 10, 2025Nicola Galvanetto, Miloš T. Ivanović, Simone A. Del Grosso, Aritra Chowdhury, Andrea Sottini, Daniel Nettels, Robert B. Best, Benjamin Schulerdescribed previously (41); human histone H1.0 was purchased from New England Biolabs (product code M2501S). Poly L-lysine hydrochloride (referred to as K50) and poly L-arginine hydrochloride (referred to as

(2) Role of charges in a dynamic disordered complex between an IDP and a folded domainNat CommunApril 4, 2025Katrine Bugge, Andrea Sottini, Miloš T. Ivanović, Freia S. Buus, Daniel Saar, Catarina B. Fernandes, Fabienne Kocher, Jacob H. Martinsen, Benjamin Schuler, Robert B. Best, et al.Unlabeled recombinant wild-type human histone H1.0 for smFRET was from New England Biolabs (cat. # M2501S) or, for NMR, produced recombinantly16. For generating the charge swap variants of GD, the following

(3) Structural mechanism of HP1⍺-dependent transcriptional repression and chromatin compactionStructureNovember 7, 2024Vladyslava Sokolova, Jacob Miratsky, Vladimir Svetlov, Michael Brenowitz, John Vant, Tyler S. Lewis, Kelly Dryden, Gahyun Lee, Shayan Sarkar, Evgeny Nudler, et al.the presence of 4-fold excess of HP1α at 37°C. For experiments containing linker histone H1.0 (NEB # M2501S), 1:1 ratio of H1:nucleosome was used. Nucleosomes with or without HP1α and/or H1.0 were mixed and incubated

(4) Mesoscale properties of biomolecular condensates emerging from protein chain dynamicsArXivJuly 27, 2024Nicola Galvanetto, Miloš T. Ivanović, Simone A. Del Grosso, Aritra Chowdhury, Andrea Sottini, Daniel Nettels, Robert B. Best, Benjamin Schulerpreparation and labeling We used recombinant human histone H1.0 (H1; New England Biolabs, product code M2501S). Poly L-lysine hydrochloride (referred to as K50, MW = 8200 Da, degree of polymerization 45–55) and

(5) Protocol for an in vitro assay to study HIV-1 Tat methylationSTAR ProtocolsDecember 1, 2023Daniela Boehm, Katrin Kaehlcke, Martina Schnoelzer, Melanie Ott,Thermo Fisher Scientific,Cat# PV4131; Lot# 1860128B "Histone H1, human, recombinant",New England Biolabs,Cat# M2501S; Lot# 0061508 "Histone H2A, human, recombinant",New England Biolabs,Cat# M2502S; Lot# 0131508 "Histone H2B, human, recombinant

(6) Structural mechanism of HP1α-dependent transcriptional repression and chromatin compactionbioRxivNovember 30, 2023Vladyslava Sokolova, Jacob Miratsky, Vladimir Svetlov, Michael Brenowitz, John Vant, Tyler Lewis, Kelly Dryden, Gahyun Lee, Shayan Sarkar, Evgeny Nudler, et al.the presence of 4-fold excess of HP1α at 37°C. For experiments containing linker histone H1.0 (NEB # M2501S), 1:1 ratio of H1:nucleosome was used. Nucleosomes with or without HP1α and/or H1.0 were mixed and incubated

(7) Extreme dynamics in a biomolecular condensateNatureJuly 27, 2023Nicola Galvanetto, Miloš T. Ivanović, Aritra Chowdhury, Andrea Sottini, Mark F. Nüesch, Daniel Nettels, Robert B. Best, Benjamin Schulerpreparation and labeling Recombinant wild-type human histone H1.0 was used (H1; New England Biolabs M2501S). ProTαC and unlabeled ProTα were prepared as previously described(Sottini et al. 2020); ProTαN cloned

(8) The lysine methyltransferase SMYD5 amplifies HIV-1 transcription and is post-transcriptionally upregulated by Tat and USP11Cell reportsMarch 28, 2023Boehm D, Lam V, Schnolzer M, Ott M.X-tremeGENE 9 DNA Transfection Reagent,Roche,Cat# XTG9-RO "Histone H1, Human, Recombinant",New England Biolabs,Cat# M2501S; Lot# 0061508 "Histone H2A, Human, Recombinant",New England Biolabs,Cat# M2502S; Lot# 0131508 "Histone H2B, Human, Recombinant

(9) Induction of broadly reactive influenza antibodies increases susceptibility to autoimmunityCell reportsMarch 8, 2022Labombarde JG, Pillai MR, Wehenkel M, Lin CY, Keating R, Brown SA, Crawford JC, Brice DC, Castellaw AH, Mandarano AH, et al.brain),Sigma,Cat# G2392 Ganglioside GM1,US Biological,Cat# G2006-10 H1 (histone),New England BioLabs,Cat# M2501S H2B (histone),New England BioLabs,Cat# M2505S H3 (histone),New England BioLabs,Cat# M2506S H4 (histone),New England BioLabs

(10) Local states of chromatin compaction at transcription start sites control transcription levelsNucleic Acids ResearchAugust 20, 2021Satoru Ishihara, Yohei Sasagawa, Takeru Kameda, Hayato Yamashita, Mana Umeda, Naoe Kotomura, Masayuki Abe, Yohei Shimono, Itoshi Nikaidosubjected to secondary electrophoresis. As a control, recombinant histones H1.0, H3.1, H2B, and H4 (#M2501S, #M2503S, #M2505S and #M2504S, respectively, New England Biolabs) were utilized. Recombinant H1.0 and

(11) Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesisCellJune 10, 2021Cui C, Chakraborty K, Tang XA, Zhou G, Schoenfelt KQ, Becker KM, Hoffman A, Chang YF, Blank A, Reardon CA, et al.62248 ClarityTM Western ECL Substrate,BIO-RAD,Cat# 170-5061 Recombinant histone H1.0,New England Biolabs,Cat# M2501S Giemsa stain solution,Millpore Sigma,Cat# R03055-74 Pierce protease inhibitor mini tablets,ThermoFisher Scientific

(12) Polyelectrolyte interactions enable rapid association and dissociation in high-affinity disordered protein complexesNature CommunicationsDecember 1, 2020Andrea Sottini et al.buffer (pH 7.0). Unlabeled recombinant wild-type human histone H1.0 was from New England Biolabs (cat. # M2501S). For experiments using biotinylated ProTα, the cDNA of the human PTMA gene (UniProt P06454) was cloned

(13) DNA Structure-Specific Cleavage of DNA-Protein Crosslinks by the SPRTN ProteaseMolecular cellOctober 1, 2020Reinking HK, Kang HS, Götz MJ, Li HY, Kieser A, Zhao S, Acampora AC, Weickert P, Fessler E, Jae LT, et al.,Thermo Scientific,Cat#NP0007 Phusion HF enzyme,NEB,Cat#M0530 UltraPure BSA,Thermo Scientific,Cat#AM2616 Histone H1° Human,NEB,Cat#M2501S Protein G,BioVision,Cat#6510 Lipofectamine 2000,Thermo Scientific,Cat#11668030 IGEPAL,Sigma,Cat#I8896 Biotin,IBA

(14) SSRP1-mediated histone H1 eviction promotes replication origin assembly and accelerated developmentNature communicationsMarch 12, 2020Falbo L, Raspelli E, Romeo F, Fiorani S, Pezzimenti F, Casagrande F, Costa I, Parazzoli D, Costanzo V.Healthcare) for 2 h on a rotating wheel at 4 °C with 1 µg recombinant histone H1.0 (New England Biolabs, M2501S), 5 µg Flag-SSRP1 or Spt16 and Flag-SSRP1 (5 µg each). Beads were washed four times with washing buffer

(15) A Robust Method for the Purification and Characterization of Recombinant Human Histone H1 VariantsBiochemistryJanuary 22, 2019Osunsade A, Prescott NA, Hebert JM, Ray DM, Jmeian Y, Lorenz IC, David Y.H1.0, we compared its migration on an SDS-PAGE alongside a commercial H1.0 (New England Biolabs, cat. #M2501S) and observed near-identical purity and migration pattern (Figure 1b). Since our purification scheme

(16) Acetylation blocks DNA damage–induced chromatin ADP-ribosylationNature Chemical BiologySeptember 1, 2018Glen Liszczak, Katharine L. Diehl, Geoffrey P. Dann, Tom W. Muirsingle-use aliquots and stored at −80 °C. Linker histone Histone H1.0 was purchased from NEB (product #: M2501S). Core histones All purified histones were characterized by RP-HPLC and ESI-MS analysis (Supplementary

(17) Cdk7 mediates RPB1-driven mRNA synthesis in Toxoplasma gondiiScientific reportsOctober 19, 2016Deshmukh AS, Mitra P, Maruthi M.His-TgMat1, TgCdk2-His, His-TgRPB1-CTD, GST-TgCdk7, GST-TgCyclinH, GST-TgMat1, MBP-TgMat1 and histone H1 (M2501S, New England Biolabs). His-TgMat1 was purified from the inclusion bodies. Antibodies and reagents Polyclonal

(18) A model for regulation by SynGAP-α1 of binding of synaptic proteins to PDZ-domain 'Slots' in the postsynaptic densityeLifeSeptember 13, 2016Walkup WG, Mastro TL, Schenker LT, Vielmetter J, Hu R, Iancu A, Reghunathan M, Bannon BD, Kennedy MB.Phosphorylation of 286 nM r-synGAP-α1 and 4.3 μM Histone H1 (New England Biolabs, Ipswich, MA; catalog no. M2501S), by CDK5/p35 (EMD Millipore, catalog no. 14-477M) or CDK5/p25 (EMD Millipore, catalog no. 14–516) was

(19) Discovery of Potent Antiproliferative Agents Targeting EGFR Tyrosine Kinase Based on the Pyrido[3′,2′:4,5]thieno[3,2-d]pyrimidin-4-amine ScaffoldCHEMICAL & PHARMACEUTICAL BULLETINDecember 4, 2015Yasmine Mohamed Abdel Aziz, Mohamed Mokhtar Said, Hosam Ahmed El Shihawy, Mai Fathy Tolba, Khaled Abouzid Mohamed AbouzidGSK3α (BPS #40006), GSK3β (BPS #40007) served as enzymes sources. While GSKtide (BPS), Histone H1 (NEB #M2501S), Poly(Glu, Tyr) sodium salt, (4 : 1, Glu : Tyr) (Sigma #P7244) served as the standardized substrate,

(20) Leukocyte protease binding to nucleic acids promotes nuclear localization and cleavage of nucleic acid binding proteinsJournal of immunology (Baltimore, Md. : 1950)June 1, 2014Thomas MP, Whangbo J, McCrossan G, Deutsch AJ, Martinod K, Walch M, Lieberman J.described (14). GST-tagged HuR, hnRNPC1, and LMNB1 were expressed and purified as described (4). H1 (NEB M2501S) and caspase 3 (Enzo – ALX-201-059-U025) were purchased. Other serine proteases were NE (Athens Research

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Histone H10Human, Recombinant

Histone H10Human, Recombinant from New England Biolabs

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New England Biolabs

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Histone H10Human, Recombinant from New England Biolabs

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