Histone H2A Human, Recombinant M2502S from New England Biolabs

Product Specs
ItemHistone H2A Human, Recombinant
CompanyNew England Biolabs
Price $95.00 Inquire
Catalog NumberM2502S
Size100 µg
Molecule NameHistone H2A
ApplicationsFunctional Genomics
TypeProteins
Source/Expression SystemRecombinant
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Extracellular Histones as Exosome Membrane Proteins Regulated by Cell StressJournal of extracellular vesiclesFebruary 1, 2025Singh B, Fredriksson Sundbom M, Muthukrishnan U, Natarajan B, Stransky S, Görgens A, Nordin JZ, Wiklander OPB, Sandblad L, Sidoli S, et al.Liposome/EV Binding Assays Human recombinant histones from New England Biolabs: H1 (M2501S), H2A (M2502S), H2B (M2505S), H3.1 (M2503S) and H4 (M2504S), total histones extracted from calf thymus (H9250, Type

(2) Arabidopsis histone acetyltransferase complex coordinates cytoplasmic histone acetylation and nuclear chromatin accessibilityScience AdvancesDecember 6, 2024Chan-Juan Wu, Xin Xu, Dan-Yang Yuan, Zhen-Zhen Liu, Lian-Mei Tan, Yin-Na Su, Lin Li, She Chen, Xin-Jian HeE. coli, were used as the enzyme sources. As substrates, we used recombinant human histones H2A (NEB, M2502S) and H4 (NEB, M2504), recombinant Arabidopsis histones (His-SUMO-H2A and His-SUMO-H4) purified from

(3) Sensitive Detection of Histones and γ-H2AX by Immunoblotting: Problems and SolutionsChemical research in toxicologySeptember 16, 2024Krawic C, Luczak MW, Zhitkovich A.proteins. Experimental Procedures Materials Individual histones were purchased from New England Biolabs (H2A: M2502S, H2B: M2505S, H3.1: M2507S, H4: M2504S), Epicypher (H2AX: 15–0307) and Sigma (H2AZ: 14–1109). Bleomycin

(4) Protocol for an in vitro assay to study HIV-1 Tat methylationSTAR ProtocolsDecember 1, 2023Daniela Boehm, Katrin Kaehlcke, Martina Schnoelzer, Melanie Ott,New England Biolabs,Cat# M2501S; Lot# 0061508 "Histone H2A, human, recombinant",New England Biolabs,Cat# M2502S; Lot# 0131508 "Histone H2B, human, recombinant",New England Biolabs,Cat# M2505S; Lot# 0031508 "Histone H3.1, human, recombinant

(5) A crucial role for dynamic expression of components encoding the negative arm of the circadian clockNature CommunicationsJune 8, 2023Bin Wang, Xiaoying Zhou, Arminja N. Kettenbach, Hugh D. Mitchell, Lye Meng Markillie, Jennifer J. Loros, Jay C. Dunlap(Sigma-Aldrich) was added to the washed resins. Recombinant human histone H2A (New England BioLabs, Catalog # M2502S) or H4 (New England BioLabs, Catalog # M2504S) respectively was added to the mixture at a final concentration

(6) The lysine methyltransferase SMYD5 amplifies HIV-1 transcription and is post-transcriptionally upregulated by Tat and USP11Cell reportsMarch 28, 2023Boehm D, Lam V, Schnolzer M, Ott M.,New England Biolabs,Cat# M2501S; Lot# 0061508 "Histone H2A, Human, Recombinant",New England Biolabs,Cat# M2502S; Lot# 0131508 "Histone H2B, Human, Recombinant",New England Biolabs,Cat# M2505S; Lot# 0031508 "Histone H3.1, Human, Recombinant

(7) The macromolecular complexes of histones affect protein arginine methyltransferase activitiesJournal of Biological ChemistryOctober 1, 2021Melody D. Fulton, Mengtong Cao, Meng-Chiao Ho, Xinyang Zhao, Y. George ZhengReaction Biology (catalog no. HMT-21-172, lot no. 1451). Human recombinant full-length histones H2A (catalog no. M2502S), H2B (catalog no. M2505S), H3.3 (catalog no. M2507S), and H4 (catalog no. M2504S) were purchased from

(8) Self-acetylation at the active site of phosphoenolpyruvate carboxykinase (PCK1) controls enzyme activityJournal of Biological ChemistryJanuary 21, 2021Pedro Latorre-Muro et al.reaction mix. The p300 acetyltransferase assays were performed by incubating either PCK1 or Histone H2A (M2502S, New England Biolabs) (both at 6 μM concentration) in the presence of p300 (6 nM) (SRP2079, Sigma) in

(9) Self-acetylation at the active site of phosphoenolpyruvate carboxykinase (PCK1) controls enzyme activityJ Biol ChemJanuary 21, 2021Pedro Latorre-Muro, Josue Baeza, Ramon Hurtado-Guerrero, Thomas Hicks, Ignacio Delso, Cristina Hernández-Ruiz, Adrian Velázquez-Campoy, Alexis J. Lawton, Jesús Angulo, John M. Denu, et al.reaction mix. The p300 acetyltransferase assays were performed by incubating either PCK1 or Histone H2A (M2502S, New England Biolabs) (both at 6 μM concentration) in the presence of p300 (6 nM) (SRP2079, Sigma) in

(10) Dual protease type XIII/pepsin digestion offers superior resolution and overlap for the analysis of histone tails by HX-MSMethodsDecember 1, 2020James Mullahoo, Terry Zhang, Karl Clauser, Steven A. Carr, Jacob D. Jaffe, Malvina Papanastasiouseparated by their (m/z) ratio (MS2). Experimental Section Materials Human recombinant histones (H2A; M2502S, H2B.1; M2505S, H3.1; M2503S and H4; M2504S) were purchased from New England BioLabs Inc (Ipswich, MA).

(11) TGFBR2 mediated phosphorylation of BUB1 at Ser-318 is required for transforming growth factor-β signalingNeoplasia (New York, N.Y.)April 1, 2020Nyati S, Gregg BS, Xu J, Young G, Kimmel L, Nyati MK, Ray D, Speers C, Rehemtulla A.mutants were cloned into pFAST-Bac plasmids and expressed along with Bub3. Recombinant human H2A (#M2502S) was obtained from New England Biolabs (NEB), 2OH-BNPP was synthesized in-house. SimplyBlue SafeStain

(12) Elevated H3K79 homocysteinylation causes abnormal gene expression during neural development and subsequent neural tube defectsNature CommunicationsDecember 1, 2018Qin Zhang et al.data as shown in Supplementary Figure 2G. HTL treatment in vitro Purified histones, including H2a (M2502S, NEB), H2b (M2505S, NEB), H3 (M2503S, NEB) and H4 (M2504S, NEB) were selected. Reactions were carried

(13) Recruitment of ubiquitin-activating enzyme UBA1 to DNA by poly(ADP-ribose) promotes ATR signallingLife science allianceJune 1, 2018Kumbhar R, Vidal-Eychenié S, Kontopoulos DG, Larroque M, Larroque C, Basbous J, Kossida S, Ribeyre C, Constantinou A.S. cerevisiae Uba1 (E-300), and human UBA6 (E-307) were acquired from Boston Biochem. Purified histone H2A (#M2502S) was obtained from New England Biolabs. Radioactive pADPr binding assay Radioactive pADPr binding

(14) Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during SpermiogenesisCellJune 1, 2017Gou LT, Kang JY, Dai P, Wang X, Li F, Zhao S, Zhang M, Hua MM, Lu Y, Zhu Y, et al.Proteins","Chemicals, Peptides, and Recombinant Proteins" "Human, Recombinant Histone H2A",New England BioLabs,Cat#M2502S "Human, Recombinant Histone H2B",New England BioLabs,Cat#M2505S Hematoxylin and Eosin,"Shanghai hongqiao

(15) Caenorhabditis elegans PRMT-7 and PRMT-9 Are Evolutionarily Conserved Protein Arginine Methyltransferases with Distinct Substrate SpecificitiesBiochemistryMay 23, 2017Andrea Hadjikyriacou, Steven G. ClarkehistonesReactions consisting of 5 μg of substrate (recombinant human histones from New England BioLabs (H2A: M2502S; H2B: M2505S; H3.3: M2507S; H4: M2504S) were reacted with 2 μg of C. elegans GST-PRMT-7 or human GST-PRMT7

(16) Competition between histone and transcription factor binding regulates the onset of transcription in zebrafish embryosElifeApril 20, 2017Shai R Joseph, Máté Pálfy, Lennart Hilbert, Mukesh Kumar, Jens Karschau, Vasily Zaburdaev, Andrej Shevchenko, Nadine L Vastenhouwwere of human origin and produced in E. Coli (NEB, Ipswich, MA; 1 mg/ml: H3.1 M2503S, H4 M2504S, H2A M2502S, H2B M2505S). We used human histones because histones are highly conserved between species and these

(17) Competition between histone and transcription factor binding regulates the onset of transcription in zebrafish embryoseLifeApril 20, 2017Shai R Joseph, Máté Pálfy, Lennart Hilbert, Mukesh Kumar, Jens Karschau, Vasily Zaburdaev, Andrej Shevchenko, Nadine L Vastenhouwwere of human origin and produced in E. Coli (NEB, Ipswich, MA; 1 mg/ml: H3.1 M2503S, H4 M2504S, H2A M2502S, H2B M2505S). We used human histones because histones are highly conserved between species and these

(18) Quantitative proteomics to characterize specific histone H2A proteolysis in chronic lymphocytic leukemia and the myeloid THP-1 cell lineInternational journal of molecular sciencesMay 27, 2014Glibert P, Vossaert L, Van Steendam K, Lambrecht S, Van Nieuwerburgh F, Offner F, Kipps T, Dhaenens M, Deforce D.(Hercules, CA, USA) and Vivaspin-2 columns from Sartorius (Göttingen, Germany). The Recombinant human H2A (M2502S) was obtained from New England Biolabs (Ipswich, MA, USA) and bovine histone extract (cat. no. 223565)

(19) Recognition of UbcH5c and the nucleosome by the Bmi1/Ring1b ubiquitin ligase complexThe EMBO JournalAugust 17, 2011Matthew L Bentley, Jacob E Corn, Ken C Dong, Qui Phung, Tommy K Cheung, Andrea G Cochranpreparation Recombinant histones H2A, H2B, H3.1, and H4 were purchased from New England Biolabs (catalogue # M2502S, M2505S, M2503S, M2504S, respectively). Recombinant human histone H2A/H2B dimer and (H3.1/H4)2 tetramer

(20) Involvement of KDM1C histone demethylase-OTLD1 otubain-like histone deubiquitinase complexes in plant gene repressionProceedings of the National Academy of Sciences of the United States of AmericaJuly 5, 2011Krichevsky A, Zaltsman A, Lacroix B, Citovsky V.H3 variant conserved in all eukaryotes, from yeast to humans (56), and H4 (New England Biolabs; M2505S, M2502S, M2507S, and M2504S, respectively), or BSA (Sigma-Aldrich; A7906). After a wash in PBS pH 7.4, the plates

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Histone H2A Human, Recombinant

Histone H2A Human, Recombinant from New England Biolabs

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New England Biolabs

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Histone H2A Human, Recombinant from New England Biolabs

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