Fig 1: CD103 limits IFN-I response in bone marrow derived dendritic cells. (A) Representative FACS plot showing CD103 expression on MHCII+CD11c+ cells from naïve BMDC culture from WT or Itgae−/− mice (n = 3). Values in the plots represents the frequency of cells within CD103 positive bar. (B) Expression of Ifna4 and Ifnb1 determined by RT-PCR in WT and Itgae−/− BMDC which were left untreated or were treated with VSV (MOI = 1 for 3 h, n = 3). (C) Representative FACS plot showing VSV-GP staining from naïve or VSV treated (MOI-0.01 for 24h) BMDC generated from WT or Itgae−/− mice (n = 3). (D, E) BMDC generated from WT and Itgae−/− mice (n = 6) incubated with VSV-WT (MOI = 1, D) or VSV- EBOV (MOI = 1, E) and after 1h media was replaced with virus free fresh complete media and virus titers measured in supernatant at indicated time points. (F) Virus titers in supernatants of WT and Itgae−/− BMDC, which were treated with anti-IFNAR antibody (40µg/ml) or isotype control antibody (40µg/ml), measured after infection with VSV (MOI = 0.001, n = 6). (G) Virus titers in supernatants of WT and Itgae−/− BMDC, which were treated with IFNa4 (100U/ml), measured after infection with VSV (MOI = 0.01, n = 6). Data are shown as mean ± SEM. n.s. not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 (Student’s t-test).