PureLink DNase Set 12185010 from Thermo Fisher Scientific

Product Specs
ItemPureLink DNase Set
CompanyThermo Fisher Scientific
Price $102.00 Inquire
Catalog Number12185010
Concentration10X

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Lavender compounds interfere with AI-2 dependent bioluminescence in Vibrio harveyi without affecting LuxS signaling in Campylobacter jejuniiScienceApril 17, 2026Blaž Jug, Dina Jug, Sonja Smole Možina, Anja KlančnikPureLink RNA mini kit,"Thermo Fisher Scientific, USA",CAT#12183018A PureLink DNase kit,"Thermo Fisher Scientific, USA",CAT#12185010 Qubit RNA HS kit,"Thermo Fisher Scientific, USA",CAT#Q32852 BacTiter-Glo reagent,"Promega, USA",CAT#

(2) The plant hormone, 6-benzylaminopurine, ameliorates obesity in male and female mice while on a high-fat dietMol MetabApril 11, 2026Calvin V. Lieu, Cindy X. Zhang, Neruja Loganathan, Leon French, Andre Krunic, Sarah B. Cash, Jin Shi, Juliette Lee, Kacey J. Prentice, Carolyn L. Cummins, et al.(Invitrogen, 12183025) according to the manufacturer’s instructions with the on-column DNase step (Invitrogen, 12185010) to remove genomic DNA. Regardless of isolation method, RNA quantity and quality were analyzed using

(3) Inflammatory responses following CRISPR modification of the nuclear localisation sequence in endogenous interleukin-1 alphaDisease Models & MechanismsApril 1, 2026Christopher Hoyle, Rodrigo Díaz Pino, Si Min Lai, Jack P. Green, Antony Adamson, Graham Coutts, Catherine B. Lawrence, Mark Travis, David Brough, Gloria Lopez-CastejonFixation/Permeabilization Concentrate and Diluent (00-5521-00), PureLink RNA mini kit (12183018A) and PureLink DNase set (12185010) were from Invitrogen. YVAD (4018838) was from Cambridge Bioscience. Superscript III Reverse Transcriptase

(4) Acid Ceramidase Inhibition Disrupts Ceramide Homeostasis and Induces Mitochondrial Apoptosis in IDH1-Mutant OligodendrogliomaResearch SquareMarch 25, 2026Mioara Larion, Helena Muley, Faris Zaibaq, Meili Zhang, Dionne Davis, Michael Kruhlak, Samarth Mathur, Adrian Lita, Hua Song, Wei Zhang, et al.(12183025, Invitrogen), including an on-column DNase digestion step using the PureLink™ DNase Set (12185010, Invitrogen). RNA Sequencing RNA integrity was assessed using a 5300 Fragment Analyzer System (Agilent

(5) The Rpd3 histone deacetylase is a critical regulator of temperature-mediated morphogenesis and virulence in the human fungal pathogen HistoplasmaPLOS BiologyMarch 17, 2026Nebat Ali, Mark Voorhies, Rosa A. Rodriguez, Anita SilmM TrisCl (pH = 7.5) in 80% ethanol. The columns were then treated with Purelink DNAse (Invitrogen 12185010) and subjected to additional washes with 3 M NaOAc (pH = 5.5) and 10 mM TrisCl (pH = 7.5) in 80% ethanol

(6) Tumor-induced orexigenic imbalance lowers protein appetite and drives early organ wasting symptomsNature CommunicationsMarch 6, 2026Afroditi Petsakou, Elizabeth Filine, Matthew Li, Yuchen Chen, Alice Zheng, Norbert Perrimon(ThermoFisher 12183018 A) to extract RNA per biological replicate, including PureLink DNase (ThermoFisher, 12185010). 500 ng of RNA was amplified and converted to cDNA using the iScript cDNA Synthesis kit (Bio-Rad, 1708890).

(7) Microcephaly-associated protein WDR62 supports purine metabolism by interacting with co-chaperone BAG2The EMBO JournalMarch 5, 2026Matthew J Morris, Yvonne Y Yeap, Jonathon R Edwards, Chi Chen, Annalisa Paolino, Sebastian G B Furness, S Sean Millard, Julia K Pagan, Laura R Fenlon, Dominic C H Ngmanufacturer’s instructions. On-column DNase treatment was performed using the PureLink™ DNase Set (Invitrogen, #12185010). Real-time PCR was performed on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems) after

(8) CD8 + T cells are primed by cDC1 and exacerbate tau-mediated neurodegenerationbioRxivMarch 1, 2026Hao Hu, Peter Bor-Chian Lin, Carisa Zeng, Prabal Sharma, Yongyi Li, Hong Jiang, Jonathan Nulman, Ray A. Ohara, Tong Wu, Shasha Li, et al.isolated by using the PureLink RNA mini kit (Invitrogen, 12183020) and PureLink DNase (Invitrogen, 12185010) according to the manufacturer’s protocol. 50 nanograms of microglial RNA from each sample were used

(9) A plastic EMP1⁺ to LGR5⁺ cell state conversion as a bypass to KRAS-G12D pharmacological inhibition in metastatic colorectal cancerCancer DiscoveryFebruary 6, 2026Alessia Centonze, Adrià-Jaume Roura, Meritxell Novillo-Font, Cristina Giordano, Xavier Hernando-Momblona, Montserrat Llanses, Paula Prats, Marta Sevillano, Débora Cabot, Mireia Novell, et al.using the PureLink RNA Mini Kit (Life Technologies, 12183025) and PureLink DNAse (Life Technologies, 12185010) following the manufacturer’s specifications. RNA was eluted using distilled water (Invitrogen, 10977-035),

(10) Depletion of individual dietary amino acids induce distinct metabolic and chromatin statesJournal of Biological ChemistryFebruary 1, 2026Spencer A. Haws, Yang Liu, Cara L. Green, Krittisak Chaiyakul, Pragyan Mishra, Reji Babygirija, Eric A. Armstrong, Anusha T. Mehendale, Irene M. Ong, Dudley W. Lamming, et al.extracted from liver with TRI Reagent (Thermo Fisher, AM9738), then treated with DNase (Invitrogen, 12185010) and purified with RNA purification kit (Ambion, 12183025). The concentration and purity of RNA was

(11) The origin of hepatocellular carcinoma depends on metabolic zonationScienceJanuary 29, 2026Jason Guo, Roger Liang, Andrew Chung, Zhijie Li, Boyuan Li, Eric Chen, Lin Li, Jingjing Wang, Meng-Hsiung Hsieh, Ivy Xiangyi Fang, et al.PureLink RNA Mini Kit”). Additionally, on-column DNAse treatment was performed with the PureLink DNase Set (Invitrogen #12185010) according to the manufacturer’s protocol (page 63, “On-column PureLink DNase Treatment Protocol) before

(12) Human Liver Organoids as an Experimental Tool to Investigate Lipocalin-2 in Hepatic InflammationCellsJanuary 23, 2026Katharina S. Hardt, Robert F. Pohlberger, Diandra T. Keller, Eva M. Buhl, Florian W. R. Vondran, Anjali A. Roeth, Ralf Weiskirchen, Sarah K. Schröder-LangeFisher Scientific) according to the manufacturer’s instructions, including additional DNase digestion (12185010, Invitrogen, Thermo Fisher Scientific). After RNA clean-up, 1 µg of RNA was reverse transcribed to complementary

(13) Microglial epigenetic memory is associated with accelerated resolution of inflammatory pain induced by prophylactic macrophage-derived small extracellular vesiclesbioRxivJanuary 22, 2026Xuan Luo, Jason R. Wickman, Jason T. DaCunza, Yuzhen Tian, Ahmet Sacan, Seena K. Ajit(#AM1561, Invitrogen) according to the manufacturer’s instructions, with on-column DNAse treatment (#12185010, Invitrogen) performed during Wash I. After RNA concentrations were determined using a NanoDrop ND1000

(14) In-depth comparison of methods to isolate RNA from common human cell linesBMC GenomicsJanuary 13, 2026Thomas Josef Zech, Robert Fürstboth types of DNase digestion. For on-column DNase digestion, the PureLink DNase set (catalog number 12185010, Thermo Fisher) was used according to the provided instructions. For off-column DNase digestion, DNase I amplification grade

(15) Optimization and Characterization of SHIP1 Ligands for Cellular Target Engagement and Activity in Alzheimer’s Disease ModelsbioRxivJanuary 5, 2026Cynthia D. Jesudason, Claudia Rangel-Barajas, Colin J. Beach, Daniel E. Beck, Isaac H. Caballero-Floran, W. Brent Clayton, Lais Da Silva, Juliane C. David, Suzanne Doolen, Alexandra N. Faulkner, et al.was isolated using the PureLink RNA mini kit (Invitrogen, 12183020) and PureLink DNase (Invitrogen, 12185010) according to the manufacturer’s protocol. RNA quality and quantity were determined using the Nanodrop 2000 spectrophotometer

(16) Astrocytic Chromatin Remodeler ATRX Gates Hippocampal Memory Consolidation Through Metabolic and Synaptic RegulationGliaJanuary 1, 2026Miguel A. Pena‐Ortiz, Julia K. Sunstrum, Alireza Ghahramani, Haley McConkey, Vanessa Dumeaux, Wataru Inoue, Nathalie G. Bérubéusing the PureLink RNA Mini Kit (cat# 12183020, Thermo Fisher Scientific) with DNAse treatment (Cat# 12185010, Thermo Fisher Scientific). PolyA+ libraries were prepared from 1.5 μm of RNA at Canada's Michael Smith

(17) Chronic Intermittent Hypoxia Exposure Induces a Unique Microglial Transcriptome in 5XFAD MiceMolecular NeurobiologyJanuary 1, 2026Kaitlyn M. Marino, Andrea C. Ewald, Jaidynne N. Lash, Erin C. McCann, Shibani Ram, Phinea Z. Romero, Tao Wang, Abigail L. Watters, Abigail B. Radcliff, Daniel C. Shippy, et al.then enzymatically digested with papain (from papaya latex, Sigma #P4762-500MG) and DNAse (Invitrogen #12185010) at 37 °C, gravity fed through filters and myelin was separated from the cells using 26% percoll solution

(18) Cannabinoid Ligand-Mediated Glycogen Depletion in Astrocytes Is Associated With Increased Intracellular Calcium, Energy Metabolism, and Membrane DynamicsJ NeurochemDecember 28, 2025Katja Fink, Robert Zorec, Marko Kreftmanufacturer's protocol, including on‐column genomic DNA removal with the PureLink DNase Set (Cat. 12185010). Samples were homogenized using the PureLink Homogenizer, 50 Rxn (Cat. 12183026). RNA concentration

(19) Endogenous retrovirus IAP forms virus-like particles and traffics across the maternal-fetal barrierbioRxivDecember 26, 2025Abby J. Bergman, Guillaume Cornelis, Julie C. Bakerand resuspended in Trizol. Total RNA was extracted with the PureLink RNA mini kit (Life Technologies 12185010) according to manufacturers protocol for samples in trizol and treated with DNAse on column to remove

(20) Molecular characterization of the murine Leydig cell lines TM3 and MLTC-1Front Endocrinol (Lausanne)December 16, 2025Sarah K. Schröder-Lange, Katharina S. Hardt, Diandra T. Keller, Manuela Pinoé-Schmidt, Eva M. Buhl, Ralf WeiskirchenPureLink™ RNA Mini-Kit (#12183018A, Invitrogen™, Thermo Fisher Scientific) including DNase digestion (#12185010, Invitrogen™, Thermo Fisher Scientific) was used to extract and purify total RNA from murine LC lines

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PureLink DNase Set from Thermo Fisher Scientific

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PureLink DNase Set from Thermo Fisher Scientific

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