Deoxynucleotide (dNTP) Solution Mix from New England Biolabs

Product Specs
ItemDeoxynucleotide (dNTP) Solution Mix
CompanyNew England Biolabs
Price $69.00 Inquire
Catalog NumberN0447S
Quantity8 µmol
Molecule NameDeoxynucleotide
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) An In Vitro A-431 Epithelial Cell Infection Model for Studying Fungal Pathogenicity and Immune Responses Associated With Vulvovaginal CandidiasisBio ProtocMay 20, 2026Victoria E. Groß, Kar On Cheng, Beatriz Cristóvão, Ezgi Gürel, Maximilian Himmel, Candela Fernández-Fernández, Jördis V. Schuchardt, Axel Dietschmann, Dolly E. Montaño, Mark S. Gresnigtcatalog number: A6002) 23. Deoxynucleotide (dNTP) solution mix (New England BioLabs Inc., catalog number: N0447S) 24. RNaseOUTTM recombinant RNase inhibitor (Invitrogen, catalog number: 10777-019) 25. SuperScriptTM

(2) T-cell protrusions enable fast, localised initiation of chimeric antigen receptor signallingThe EMBO JournalApril 21, 2026Carmen Rodilla-Ramirez, Giorgia Carai, Eleanor Fox, Amin Zehtabian, Helen Adam, Katja Dallio, Pia Lazki-Hagenbach, Helge Ewers, Xiaolei Su, Francesca Bottanelli(BSA),Roth,8076.4 Complete EDTA-free Protease Inhibitor,Sigma-Aldrich,4693132001 deoxynucleotide triphosphates (NTPs),NEB,N0447S "Dulbecco’s Phosphate Buffered Saline (D-PBS), 10x",Corning,20-031-CV EDTA,Biozym Scientific,831234 EGTA,abcr,AB304133 Ethanol,Roth,5054.4 Foetal

(3) Genomic diversity analysis enables development of pan-Dengue Toehold RNA sensorsPLoS Negl Trop DisApril 16, 2026Anirudh Nandakumar, Deeksha Mishra, Akshay Shendre, Edrea Mendonca, Soujanya Nagendra, Akash Gulyani, Arati Ramesh1.6 μl of 50 mM MgCl2, 2 μl of 25 mM rNTPs (NEB, Cat. no. N0450S), 2 μl of 10 mM dNTPs (NEB, Cat. no. N0447S), 0.18 μl of 1 M DTT (VWR, Cat. no. 3483-12-3), 3 μl of 100% DMSO (Sigma-Aldrich, Cat. no. D8418-50

(4) A platform for multimodal in vivo pooled genetic screens reveals regulators of liver functionbioRxivApril 13, 2026Reuben A. Saunders, William E. Allen, Xingjie Pan, Jaspreet Sandhu, Jiaqi Lu, Thomas K. Lau, Karina Smolyar, Zuri A. Sullivan, Catherine Dulac, Jonathan S. Weissman, et al.P9416 SplintR Ligase,NEB,Cat# M0375S NxGen phi29 DNA Polymerase,Lucigen,Cat# 30221-3 dNTP Mix,NEB,Cat# N0447S Aminoallyl-dUTP,ThermoFisher,Cat# R1101 T4 gene 32 Protein,NEB,Cat# M0300L BccI,NEB,Cat# R0704S BciVI,NEB,Cat#

(5) Sequencing complete plasmids on Oxford Nanopore Technologies sequencers using R2C2 and ChopperPLoS OneApril 10, 2026Kayla D. Schimke, Christopher Vollmerseach: [5 uL Phi29 Buffer (10X,NEB, M0269S), 1 uL Phi29 Polymerase(NEB, M0269S), 2.5 uL dNTP (10 mM;NEB N0447S), 2.5 uL Random hexamer primers (10 uM, Thermo, SO181), 10 uL plasmid (10ng/ul), 29 uL ultra-pure water].

(6) MK2/p38/p53 Suppress Basal IL-1β and Non-Canonical NF-κB Signaling in MacrophagesInt J Mol SciApril 2, 2026Sarah M. Herr, Diana Stalkopf, Sofie Padaszus, Lukas A. Herbst, Anneke Dörrie, Rainer Niedenthal, Natalia Ronkina, Tatiana Yakovleva, Alexey Kotlyarov, Matthias Gaestel(all from Thermo Fisher Scientific Inc., Waltham, MA, USA), and Deoxynucleotide (dNTP) Solution Mix (N0447S, New England Biolabs GmbH, Frankfurt am Main, Germany). The cDNA was diluted 1:10, and quantitative PCR

(7) Phage-based diagnostic assay for melioidosis using multiplex PCR targeting Burkholderia phage terminase genesiScienceMarch 20, 2026Patoo Withatanung, Muthita Vanaporn, Narisara Chantratita, Sorujsiri Chareonsudjai, Veerachat Muangsombut, Sujintana Janesomboon, Thatchanon Asawalertsaeng, Martha R.J. Clokie, Edouard E. Galyov, Sunee Korbsrisate"Thermo Fisher Scientific, USA",Cat# S33102 Deoxynucleotide (dNTP) Solution Mix,"New England BioLabs, USA",Cat# N0447S UltraPure™ BSA (50 mg/mL),Thermo Fisher Scientific,Cat# AM2616 "DEPC-Treated Water, 5 x 100 mL",Thermo Fisher Scientific

(8) Regulation of YAP activity by nuclear G-actin bindingNucleic Acids ResMarch 19, 2026Hong Wang, Irma M Jayawardana, Justus M Fleisch, Dennis Frank, Bing Zhao, Christos Kamaras, Peter Gebhardt, Julian Knerr, Svenja Ulferts, Shamphavi Sivabalasarma, et al.total RNA, 2 µL Oligo d(T)23 VN (New England Biolabs, S1327S), 1 µL 10 mM dNTP (New England Biolabs, N0447S) for 5 min at 65°C, then cDNA synthesis was synthesized at 42°C for 1 h after adding 2.8 µL Nuclease-free

(9) A One-Pot CRISPR/Cas12a-Based Platform for Contamination-Free Nucleic Acid Amplification DetectionBiosensors (Basel)March 19, 2026Wei Tantai, Qinfeng Xu, Wenjuan Zhang, Yanni Li, Hao Liuand the LAMP reagents, including Bst 2.0 WarmStart DNA polymerase (M0538S), MgSO4 (B1003S), dNTPs (N0447S), and 10× LAMP amplification buffer (2 mM MgSO4, 10 mM (NH4)2SO4, 50 mM KCl, 20 mM Tris-HCl, 0.1% Tween-20),

(10) Protocol for detecting in vitro riboswitch conformational switching using a fluorescence anisotropy single-stranded RNA-targeting approachSTAR ProtocMarch 19, 2026Amanda Clairoux, Maira Rivera, Maureen McKeague, Anthony MittermaierPhusion™ High-Fidelity DNA Polymerase,New England Biolabs,M0530S Deoxynucleotide (dNTP) Solution Mix,New England Biolabs,N0447S ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, MEGAshortscript™ T7 Transcription Kit

(11) Interdependent RNA structural motifs at the 3ʹ-terminus of the West Nile virus genome regulate viral growthbioRxivMarch 14, 2026Lucille H. Tsao, Doug E. Brackney, Anna Marie Pyle(100 mM Tris-HCl pH 8.3, 400 mM KCl, 4 mM MgCl2, 10 mM DTT, 40% glycerol) and 0.5 mM dNTP (NEB, cat #N0447S). RT reactions were incubated at 42°C for 30 minutes. RNA was then digested with 0.5 ul of RNase T1

(12) DNA Adenine Methylation Clock in Brain Aging and Alzheimer’s Disease ProgressionbioRxivMarch 11, 2026Abdur Rahim, Xiaomei Zhan, Qiyuan Han, Allysha O’Donnell, Angela Jeong, Guru-Swamy Madugundu, Suresh Pujari, Monica Kruk, Xinlong Luo, Ling Li, et al.tagging method (PBAT).30 Briefly, to the nitrite-treated DNA solution, we added 1.25μL 10mM dNTPs (NEB, N0447S), 4μL 100μM Biotin-R2-N9 primer (5’-[Btn]CAGACGTGTGCTCTTCCGATCTNNNNNNNNN-3’), 5 μL 10X DNA polymerase

(13) RNA localization to nuclear speckles follows splicing logicNucleic Acids ResMarch 6, 2026Li Wen, Mauricio A Arias, Xinqi Fan, Joyce Xie, Sneha Paul, Susan E Liao, Marek Sobczyk, Oded Regev, Jingyi Feiconstruct-specific reverse primer (Supplementary Table S1), 10% DMSO (Thermo Fisher, BP231), 1 mM dNTPs (NEB, N0447S), and 10 mM DTT (Sigma–Aldrich, 10197777001). Reverse transcriptase from iScript cDNA synthesis kit

(14) Protocol for targeted gene manipulation and thermogenic evaluation in mouse brown adipocytesSTAR ProtocolsMarch 1, 2026Jakub Bunk, Drishti Soni, Matthias Calderon, Bozena Samborska, Lawrence Kazakproteins" ,, PfuTurbo DNA Polymerase,Agilent,600250 Deoxynucleotide (dNTP) Solution Mix,New England Biolabs (NEB),N0447S HindIII-HF,New England Biolabs (NEB),R3104S SalI-HF,New England Biolabs (NEB),R3138S 10× rCutSmart Buffer

(15) Analysis and control of untemplated DNA polymerase activity for guided synthesis of kilobase-scale DNA sequencesNature CommunicationsFebruary 26, 2026Simeon. D. Castle, Thea C. T. Irvine, Adrian Woolfson, Gregory Linshiz, Blake T. Riley, Ifor D. W. Samuel, Loren Picco, Philipp Holliger, Lauren M. Oldfield, Andrew Hessel, et al.or 96-well PCR plates, using reaction volumes of 100 µL with 200 µM of dNTPs (New England Biolabs, N0447S). Reactions were prepared on ice in a PCR hood before being transferred to a preheated thermocycler

(16) A non-catalytic role for RFC in PCNA-mediated processive DNA synthesisCellFebruary 19, 2026Gabriella N. L. Chua, Emily C. Beckwitt, Victoria Miller-Browne, Olga Yurieva, Dan Zhang, Bryce J. Katch, Nina Y. Yao, John W. Watters, Kaitlin Abrantes, Ryogo Funabiki, et al.R3133S ATP solution,ThermoFisher,Cat# R0441 Deoxynucleotide (dNTP) Solution Mix,New England Biolabs,Cat# N0447S TCEP-HCl (Tris(2-carboxyethyl)phosphine hydrochloride),Goldbio,Cat# TCEP1 DTT (Dithiothreitol) (> 99% pure) protease free

(17) A portable and low-cost fluorescence reader for near-patient nucleic acid amplification assaysBiomed MicrodevicesFebruary 14, 2026Ethan Rosenfeld, Kathryn Pacheco, Evan Benke, Ian M. White, Don L. DeVoeisothermal amplification buffer (B0537S, New England Biolabs), trehalose (15% by weight), 0.6 mM dNTPs (N0447S, New England Biolabs), 1X EvaGreen intercalating dye (Biotium), 0.32 U/uL BST 2.0 DNA polymerase (New

(18) Sequencing DNA methylation and hydroxymethylation at co-occurring chromatin featuresNature CommunicationsFebruary 10, 2026Rafael de Cesaris Araujo Tavares, Somdutta Dhir, Xuan He, Jack Monahan, Minna Taipale, Paula Golder, Aldo Ciau-Uitz, Walraj Gosal, David Tannahill, Shankar Balasubramanianand 320 U Taq DNA ligase (M0208S), in 1X Taq DNA ligase reaction buffer containing 0.8 mM dNTPs (NEB N0447S) at 37 °C for one hour. DNA was purified on Zymo columns and digested in exonuclease digestion mix (1X

(19) Electrostatic properties of disordered regions control transcription factor search and pioneer activityNature CommunicationsFebruary 8, 2026Sim Sakong, Beat Fierz, David M. Suterof a fluorescently labeled primer, a 1.1–1.2 molar equivalent of a second primer, 200 µM dNTPs (NEB, N0447S), and 1–2 U of Phusion DNA polymerase (NEB, M0530S). The two primers were either conjugated with AF647

(20) Integrative Single-Cell Epigenomic Atlas Annotates the Regulatory Genome of the Adult Mouse BrainbioRxivFebruary 7, 2026Zhaoning Wang, Songpeng Zu, Ethan J. Armand, Timothy H. Loe, Jonathan A. Rink, Wanying Wu, Yang Xie, Lei Chang, Chenxu Zhu, Nicholas D. Johnson, et al.in 24 μL RT mix [6 μL 5x RT buffer, 6.9375 μL nuclease-free H2O, 6 μL PBS, 1.5 μL 10 mM dNTPs (NEB, N0447S), 0.375 μL SUPERase Inhibitor, 0.1875 μL RNase OUT, 3 μL Maxima H minus reverse transcriptase (ThermoFisher Scientific

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