Deoxynucleotide (dNTP) Solution Mix N0447L from New England Biolabs

Product Specs
ItemDeoxynucleotide (dNTP) Solution Mix
CompanyNew England Biolabs
Price $274.00 Inquire
Catalog NumberN0447L
Molecule NameDeoxynucleotide
ApplicationsDNA Analysis
Temperature-20 C
Size40 µmol

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Increasing usable reads in RNA-seq protocolsiScienceAugust 21, 2026Felix Pförtner, Eva Briem, Wolfgang Enard, Daniel RichterEB,Qiagen,Cat#19086 Buffer RLT Plus,Qiagen,Cat#1053393 Deoxynucleotide Solution Mix,New England Biolabs,Cat#N0447L DNase I (RNase-free),Thermo Fisher Scientific,Cat#EN0525 DNase I Reaction Buffer,Thermo Fisher Scientific,Cat#EN0525 EDTA,Sigma-Aldrich,Cat#E7889 Ethanol

(2) Locus-Scale Massively Parallel Reporter AssaysbioRxivJuly 28, 2026Abby V. McGee, Carina G. Biar, Beth K. Martin, Tony Li, Jean-Benoît Lalanne, Haedong Kim, Jay Shendureeach reaction, 5 μg RNA was mixed with 1 μl 2 μM P7-pLSmp-assUMI-gfp and 1 μl of 10 mM dNTPs (NEB, N0447L), incubated at 65 °C for 5 min, and placed on ice. 6 μl of reverse transcription master mix was then

(3) A simple and versatile fluorescence-based method to enhance prime editing in human pluripotent stem cellsBMC MethodsJuly 15, 2026Carlye Frisch, Salma Leyasi, William W. Kostes, Stefan J. Tekel, Kylie Standage-Beier, Gayathri Srinivasan, Xiao Wang, David A. Brafman(Life Technologies 51985034)Phusion™ High-Fidelity DNA polymerase (NEB M0530L)DMSO (NEB M0530L)dNTPs (NEB N0447L)GC Buffer (NEB B0519S)Agarose (Sigma A9539)50× TAE (VWR 97063-692)SYBR Safe DNA Gel Stain (Thermo Fisher Scientific

(4) Single-bacterium RNA-seq protocol to uncover heterogeneous expression of coding and noncoding genes in Bacteroides thetaiotaomicronSTAR ProtocJuly 1, 2026Elise Bornet, Alexander J. Westermannbuffer,New England Biolabs,M0259L DNase I,Thermo Fischer Scientific,EN0521 dNTPs,New England Biolabs,N0447L Ethanol Absolute for analysis,Merck,1.00983.2511 Glucose,Merck,1.08337.1000 Hemin,Carl Roth,7629.2 L-cysteine,Carl

(5) Induction of multiple HIV-1 neutralizing B Cell Precursors in HumansmedRxivJune 30, 2026Chen-Hao Yeh, Stephen R. Walsh, Ruth J. Parsons, Qianyi E. Zhang, Bhishem Thakur, Kijun Song, Kejun Liu, Helene Fradin Kirshner, Elizabeth Van ltallie, Matthew Clark, et al.template-switching technology to enable unbiased 5’ capture. Briefly, RNA was combined with dNTPs (NEB, Cat. #N0447L), a template-switching oligonucleotide (TSO; containing 5’ padding and a plate-level barcode), and constant-region

(6) Image-based, pooled phenotyping reveals multidimensional, disease-specific variant effectsCellJune 25, 2026Sriram Pendyala, Katie Partington, Nicholas Bradley, Abbye E. McEwen, Gwenneth Straub, Hyeon-Jin Kim, Shawn Fayer, Daniel Lee Holmes, Katherine A. Sitko, Riddhiman K. Garge, et al.reaction mix containing final concentrations of 1x Revertaid RT buffer, 250 μM dNTPs (New England Biolabs, N0447L), 400 μg/mL Ultrapure BSA (Thermo Scientific, AM2616), 0.5 U/μL RiboLock (Thermo Scientific, EO0381),

(7) Enhancement of RNA Imaging Platforms by the Use of Peptide Nucleic Acid-Based LinkersBio ProtocJune 20, 2026Aleksandra J. Wierzba, Erin M. Richards, Shelby R. Lennon, Robert T. Batey, Amy E. Palmercatalog number: 403140100) 7. Deoxynucleotide (dNTP) solution mix (New England Biolabs, catalog number: N0447L) 8. Dideoxynucleotide triphosphates (ddNTPs) (Millipore Sigma, catalog number: 3732738001) 9. Dimethyl sulfoxide (DMSO

(8) Protocol for massively parallel RNA assay combined with immunoprecipitation for high-throughput analysis of RNA-protein interactions in cellsSTAR ProtocJune 19, 2026Yu Hsuan Lee, John L. Rinn, Taeyoung Hwangproteins","Chemicals, peptides, and recombinant proteins" ,, Acetylated BSA,Invitrogen,AM2614 dNTP Mix,NEB,N0447L Ultrapure Agarose,Invitrogen,16500100 TAE Buffer (50X),Quality Biological,351-008-131 LB Broth (Miller),Sigma-Aldrich,L2542 Ampicillin

(9) High-throughput functional profiling and evolutionary covariation analysis of entire riboswitch sequencesNucleic Acids ResJune 17, 2026Laura M Hertz, Anibal Arce, Elena Rivas, Julius B Luckspolymerase chain reaction (PCR) was set up to amplify 20 ng of DNA: 1 µl of ssDNA pool (20 ng/µl), dNTPS (NEB, N0447L) to final concentration of 200 µM each, Q5 Reaction Buffer (NEB, B9027S) to a final concentration of

(10) Semirandom DNA adducts regulate a filamentous defense-associated reverse transcriptaseNat Struct Mol BiolJune 10, 2026Nolan Neville, Nicole V. Johnson, Edwin E. Escobar, Chang-Hwa Chiang, Albana Nreca, Sean R. Johnson, Nan Dai, Andy Hanneman, Ivan R. Corrêa, Jason S. McLellan, et al.mM MgCl2, 2 mM DTT, 0.4 mg ml−1 RNase A (NEB, T3018L) and 0.1 mM final concentration dNTP mix (NEB, N0447L) or an equivalent volume of water were reacted for 15 min at 37 °C, then 2.2 µl of ExoI reaction buffer

(11) Embryo-scale Visual Cell Sorting reveals a conserved transcriptomic signature of nucleolar size linked to proteostasisbioRxivMay 28, 2026Hyeon-Jin Kim, Sriram Pendyala, Josephine Lin, Tony Cooke, Gang Li, William Stafford Noble, Xinxian Deng, Christine M. Disteche, Cole Trapnell, Douglas M. Fowlerwashes, 200 μL of reverse transcription mix (40 μL 5X RT buffer, 8 uL 10mM dNTPs (New England Biolabs, N0447L), 20 μL 40 μM well-specific RT primers, 10 μL RNAseOUT (Thermo Scientific, 10777019), 8 μL Maxima H

(12) Ribosome Molecular Aging Shapes Translation DynamicsbioRxivApril 25, 2026Jordy F. Botello, Lifei Jiang, Peter J. Metzger, Troy J. Comi, Aya A. Abu-Alfa, Qiwei Yu, Margaret S. Ebert, Minkyu Lee, Lennard W. Wiesner, Maya Butani, et al.previously67: 4 μL 5X SuperScript IV RT buffer (ThermoFisher, 18090050), 2 μL 10 mM dNTP mix (NEB, N0447L), 1 μL 100 mM DTT (ThermoFisher, D1532), 0.5 μL RNase inhibitor (NEB, M0314L), 1 μL SuperScript IV (200

(13) Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducensNucleic Acids ResApril 24, 2026Benjamin F Jepson, Eric J Wolf, Anthony R Dawson, Jingmin Jin, Vladimir Potapov, Nan Dai, Ivan R Corrêa Jr., Erbay YigitMgSO4, 1% Tween 20, pH 8.8 at 25°C), an additional 0.6 μl 100 mM MgSO4, 1.4 μl 10 mM dNTP solution (NEB, N0447L), and 2.75 μl of water in a total volume of 8 μl. Samples were heated at 65°C for 3 min, cooled at room

(14) The one-week automated genome-wide optical pooled screenbioRxivApril 22, 2026Bryce Kirby, Matteo Di Di Bernardo, Iain M. Cheeseman, Paul C. Blaineycarried out in 1X RevertAid RT buffer (Thermo Fisher Scientific EP0452) containing 250 μM dNTPs (NEB N0447L), 1 μM biotinylated reverse-transcription primer (Integrated DNA Technologies), 200 μg/mL recombinant

(15) Profiling KRAS mutations in whole blood by error-corrected maximum depth sequencingnpj Precision OncologyApril 3, 2026Ryne C. Ramaker, Yuchen Zhang, John H. Strickler, Peter Allen, James Abbruzzese, Christopher M. CounterStart High-Fidelity Polymerase (NEB Catalogue # M0493L), 10 µL of Q5 buffer, 4 µL of 2.5 mM dNTP mix (N0447L), and 2.5 µL of 10 µM barcoding primer encoding the strand-specific priming site, a 1-7 base pair stagger,

(16) Sp1 mechanotransduction regulates breast cancer cell invasion in engineered viscoelastic extracellular matricesBiomaterialsApril 1, 2026Abhishek Sharma, Rowan F. Steger, Jen M. Li, Samuel Y. Fong, Neha Saxena, Jane A. Baude, Kellie A. Heom, Siddharth S. Dey, Ryan S. Stowerswas reverse-transcribed using the CelSeq2 RT-primer, DTT (0.1 M), dNTPs (New England Biolabs, Cat# N0447l), and Superscript II reverse transcriptase (Invitrogen, Cat# 18064014), followed by second-strand synthesis

(17) A single cell transcriptional profile of benign prostatic hyperplasiaSci RepMarch 14, 2026Rei Unno, Jon Akutagawa, Hanbing Song, Keliana Hui, Yih-An Chen, Julia H. Pham, Jean Lee, Heiko Yang, Franklin W. Huang, Thomas Chireverse transcription mastermix with Maxima RT buffer, PEG8000, Template Switch Oligo, dNTPs (NEB, Cat: N0447L), RNase inhibitor (Life Technologies, Cat: AM2696) and Maxima H Minus Reverse Transcriptase (ThermoFisher,

(18) Mesoscale molecular architecture of the human striatum across cell types and lifespanbioRxivMarch 5, 2026Andrew W. Kraft, Matthew Lee, Nirmala Rayan, Haoyuan Gao, Julianna Milidantri, Charles Vanderburg, Karol Balderrama, Naeem Nadaf, Vipin Kumar, Katelyn Flowers, et al.SSC wash, arrays were incubated in an extension buffer (1× NEBuffer 2 [NEB, B7002S], 1 mM dNTPs [NEB, N0447L], and 0.15 U/μL Klenow Exo− [NEB, M0212L]) at 37 °C for ≥1 h. Arrays were washed twice more with 2×

(19) Rapid disassembly and Piwi-independent reassembly of Drosophila piRNA cluster heterochromatin following acute heat shockRNAMarch 1, 2026Nicholas P. Rice, Samantha Ho, Zhiping Weng, William E. Theurkauf,Thermo Fisher Scientific,Cat# 10297018 dUTP solution (100 mM),Thermo Fisher Scientific,Cat# R0133 dNTP mix,NEB,Cat# N0447L DNA polymerase I,NEB,Cat# M0209S T4 DNA polymerase,NEB,Cat# M0203L Klenow DNA polymerase,NEB,Cat#

(20) Aub, Vasa, and Armi concentration in phase-separated nuage is dispensable for piRNA biogenesis and transposon silencingRNAMarch 1, 2026Samantha Ho, Nicholas P. Rice, Tianxiong Yu, Zhiping Weng, William E. Theurkauf,Thermo Fisher Scientific,Cat# 10297018 dUTP solution (100 mM),Thermo Fisher Scientific,Cat# R0133 dNTP mix,NEB,Cat# N0447L DNA polymerase I,NEB,Cat# M0209S T4 DNA polymerase,NEB,Cat# M0203L Klenow DNA polymerase,NEB,Cat#

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Deoxynucleotide (dNTP) Solution Mix

Deoxynucleotide (dNTP) Solution Mix from New England Biolabs

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New England Biolabs

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Deoxynucleotide (dNTP) Solution Mix from New England Biolabs

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