Deoxynucleotide (dNTP) Solution Mix N0447L from New England Biolabs

Product Specs
ItemDeoxynucleotide (dNTP) Solution Mix
CompanyNew England Biolabs
Price $274.00 Inquire
Catalog NumberN0447L
Size40 µmol
Molecule NameDeoxynucleotide
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Protocol for assessing protein expression levels and localization during fission yeast cytokinesisSTAR ProtocSeptember 17, 2026Jack R. Gregory, Yanfang Ye, Davinder Singh, Aysha H. Osmani, Jian-Qiu Wu,Gold Bio,N-500–100 CuSO4 · 5H2O,Sigma-Aldrich,209198 Dextrose,Sigma-Aldrich,G8270 dNTP,New England Biolabs,N0447L Dynabeads,Invitrogen,10004D EDTA,Sigma,E6758 FeCl3 · 6H2O,Fluka,44944 G418 (Geneticin),Gibco,11811–031 Gelatin,Sigma-Aldrich,G2500 Glacial

(2) Resource-efficient Strand-seq library preparation in microliter volumes: a one-pot-style adaptation for standard laboratory infrastructureMethodsXSeptember 10, 2026Eva Benito, Ferris Jung, Catherine Stober, Patrick Hasenfeld, Maise Gomes Queiroz, Jan O. KorbelDTT,Sigma-Aldrich 43816,3.57 200mM ATP,ThermoFisher J61125.06,2.4 100mM dATP,NEB N0440,3.57 10mM dNTP,NEB N0447L,1.78 T4 DNA polymerase⁎,NEB M0203L,3.22 T4 PNK⁎,NEB M0201L,10.77 Taq⁎,NEB M0273,1.99 H2O,Ambion

(3) Massively Parallel In Vitro Functional Analysis of Evolution-Derived Transcriptional Riboswitch SequencesBio ProtocSeptember 5, 2026Laura M. Hertz, Julius B. LucksIDT) Reagents 1. RNase/DNase-free water (Invitrogen, catalog number: 10977-015) 2. dNTPs (NEB, catalog number: N0447L) 3. 5× Q5 reaction buffer (NEB, catalog number: B9027S) 4. Q5 polymerase (NEB, catalog number: M0491L) 5.

(4) Age- and sex-dependent heterogeneity during LPS-induced murine acute lung injuryImmunity & AgeingAugust 26, 2026Chantal Crispens, Annett Wilken-Schmitz, Valentin Dern, Abd Rahman Rahimi, Enrico Madau, Nadine Chiara Frigger, Josefine Jakob, Björn Häupl, Thomas Oellerich, Andreas Weigert, et al.(Sigma-Aldrich; 9068–38-6), nonamers (Sigma-Aldrich; 1003430367), dNTPs (New England BioLabs Inc.; N0447L) and RNA inhibitor (New England BioLabs Inc.; M0314L). RT-qPCR was performed with Sybr Select Mastermix,

(5) Increasing usable reads in RNA-seq protocolsiScienceAugust 21, 2026Felix Pförtner, Eva Briem, Wolfgang Enard, Daniel RichterEB,Qiagen,Cat#19086 Buffer RLT Plus,Qiagen,Cat#1053393 Deoxynucleotide Solution Mix,New England Biolabs,Cat#N0447L DNase I (RNase-free),Thermo Fisher Scientific,Cat#EN0525 DNase I Reaction Buffer,Thermo Fisher Scientific,Cat#EN0525 EDTA,Sigma-Aldrich,Cat#E7889 Ethanol

(6) RNA structures regulate norovirus life cycle and enable rational attenuation in vivoCellAugust 19, 2026Tanja Hann, Arya B. Ökten, Renata B. Filler, Craig B. Wilen, Anna Marie PyleCrystal violet,ThermoFisher,C6158 Tris base,Sigma-Aldrich,252859 KCl,Sigma-Aldrich,P3911 DTT,Sigma-Aldrich,D0632 dNTP,NEB,N0447L Random Primer 6,NEB,S1230S Manganese(II) chloride solution,Sigma-Aldrich,M1787 Tween-20,Sigma-Aldrich,P1379 TRIzol,ThermoFisher,15596018 Chloroform:Isoamyl

(7) Protein Depletion in Caenorhabditis elegans Using the Auxin-Inducible Degradation SystemMethods in Molecular BiologyAugust 10, 2026Jordan D. WardNuclease-free water (not DEPC treated; Ambion #AM9937)5X Phusion Green HF Reaction Buffer (Thermo F518L)dNTPs (NEB N0447L)Phusion (homemade; can also purchase from Thermo Scientific (#F530) or UC Berkeley Macrolabs (https://qb3.berkeley.edu/facility/qb3-macrolab/#facility-rates))AMPureXP

(8) A scalable, low-cost, sample hashing workflow for multiomic single-cell analysis using the Seq-Well S3 platformNature ProtocolsAugust 1, 2026Daniela D. Russo, Sarah L. Quinn, Olha Kholod, Michal A. Elovitz, Douglas A. Lauffenburger, Pardis Sabeti, Boris Julg, Andrea G. Edlow, Brittany A. Goods, Alex K. Shalek, et al.guidelines when disposing.MgCl2 (Sigma, cat. no. 63069-100ML)dNTP (10mM) (New England Biolabs, cat. no. N0447L)SuperaseIN RNAse Inhibitor (Thermo Fisher, cat. no. AM2694)Maxima H-RT and 5× buffer (Thermo Fisher,

(9) Locus-Scale Massively Parallel Reporter AssaysbioRxivJuly 28, 2026Abby V. McGee, Carina G. Biar, Beth K. Martin, Tony Li, Jean-Benoît Lalanne, Haedong Kim, Jay Shendureeach reaction, 5 μg RNA was mixed with 1 μl 2 μM P7-pLSmp-assUMI-gfp and 1 μl of 10 mM dNTPs (NEB, N0447L), incubated at 65 °C for 5 min, and placed on ice. 6 μl of reverse transcription master mix was then

(10) A simple and versatile fluorescence-based method to enhance prime editing in human pluripotent stem cellsBMC MethodsJuly 15, 2026Carlye Frisch, Salma Leyasi, William W. Kostes, Stefan J. Tekel, Kylie Standage-Beier, Gayathri Srinivasan, Xiao Wang, David A. Brafman(Life Technologies 51985034)Phusion™ High-Fidelity DNA polymerase (NEB M0530L)DMSO (NEB M0530L)dNTPs (NEB N0447L)GC Buffer (NEB B0519S)Agarose (Sigma A9539)50× TAE (VWR 97063-692)SYBR Safe DNA Gel Stain (Thermo Fisher Scientific

(11) Retargeted adenoviruses for local IgA and CD47 blocker production as a novel cancer therapyEMBO Molecular MedicineJuly 7, 2026Mariya Chernyavska, K Patricia Hartmann, J H Marco Jansen, Niklas Baumann, Jonas Kolibius, Dominik Brücher, Theodora Kristoforus, Rens H W Peters, Lucas Huijs, Daphne Laarveld, et al.used for cDNA synthesis. After incubation with random primers (#C1181, Promega) and deoxynucleotides (#N0447L, New England BioLabs) at 65 °C for 5 min, a mix of RNAse inhibitor (#M0307S, New England BioLabs), dithiothreitol (DTT

(12) A one-week automated genome-wide optical pooled screen using OttoSeqGenome BiologyJuly 3, 2026Bryce Kirby, Matteo Di Bernardo, Iain M. Cheeseman, Paul C. Blaineycarried out in 1X RevertAid RT buffer (Thermo Fisher Scientific EP0452) containing 250 µM dNTPs (NEB N0447L), 1 µM biotinylated reverse-transcription primer (Integrated DNA Technologies), 200 µg/mL recombinant

(13) Single-bacterium RNA-seq protocol to uncover heterogeneous expression of coding and noncoding genes in Bacteroides thetaiotaomicronSTAR ProtocJuly 1, 2026Elise Bornet, Alexander J. Westermannbuffer,New England Biolabs,M0259L DNase I,Thermo Fischer Scientific,EN0521 dNTPs,New England Biolabs,N0447L Ethanol Absolute for analysis,Merck,1.00983.2511 Glucose,Merck,1.08337.1000 Hemin,Carl Roth,7629.2 L-cysteine,Carl

(14) Induction of multiple HIV-1 neutralizing B Cell Precursors in HumansmedRxivJune 30, 2026Chen-Hao Yeh, Stephen R. Walsh, Ruth J. Parsons, Qianyi E. Zhang, Bhishem Thakur, Kijun Song, Kejun Liu, Helene Fradin Kirshner, Elizabeth Van ltallie, Matthew Clark, et al.template-switching technology to enable unbiased 5’ capture. Briefly, RNA was combined with dNTPs (NEB, Cat. #N0447L), a template-switching oligonucleotide (TSO; containing 5’ padding and a plate-level barcode), and constant-region

(15) Image-based, pooled phenotyping reveals multidimensional, disease-specific variant effectsCellJune 25, 2026Sriram Pendyala, Katie Partington, Nicholas Bradley, Abbye E. McEwen, Gwenneth Straub, Hyeon-Jin Kim, Shawn Fayer, Daniel Lee Holmes, Katherine A. Sitko, Riddhiman K. Garge, et al.reaction mix containing final concentrations of 1x Revertaid RT buffer, 250 μM dNTPs (New England Biolabs, N0447L), 400 μg/mL Ultrapure BSA (Thermo Scientific, AM2616), 0.5 U/μL RiboLock (Thermo Scientific, EO0381),

(16) Enhancement of RNA Imaging Platforms by the Use of Peptide Nucleic Acid-Based LinkersBio ProtocJune 20, 2026Aleksandra J. Wierzba, Erin M. Richards, Shelby R. Lennon, Robert T. Batey, Amy E. Palmercatalog number: 403140100) 7. Deoxynucleotide (dNTP) solution mix (New England Biolabs, catalog number: N0447L) 8. Dideoxynucleotide triphosphates (ddNTPs) (Millipore Sigma, catalog number: 3732738001) 9. Dimethyl sulfoxide (DMSO

(17) Protocol for massively parallel RNA assay combined with immunoprecipitation for high-throughput analysis of RNA-protein interactions in cellsSTAR ProtocJune 19, 2026Yu Hsuan Lee, John L. Rinn, Taeyoung Hwangproteins","Chemicals, peptides, and recombinant proteins" ,, Acetylated BSA,Invitrogen,AM2614 dNTP Mix,NEB,N0447L Ultrapure Agarose,Invitrogen,16500100 TAE Buffer (50X),Quality Biological,351-008-131 LB Broth (Miller),Sigma-Aldrich,L2542 Ampicillin

(18) High-throughput functional profiling and evolutionary covariation analysis of entire riboswitch sequencesNucleic Acids ResJune 17, 2026Laura M Hertz, Anibal Arce, Elena Rivas, Julius B Luckspolymerase chain reaction (PCR) was set up to amplify 20 ng of DNA: 1 µl of ssDNA pool (20 ng/µl), dNTPS (NEB, N0447L) to final concentration of 200 µM each, Q5 Reaction Buffer (NEB, B9027S) to a final concentration of

(19) Semirandom DNA adducts regulate a filamentous defense-associated reverse transcriptaseNat Struct Mol BiolJune 10, 2026Nolan Neville, Nicole V. Johnson, Edwin E. Escobar, Chang-Hwa Chiang, Albana Nreca, Sean R. Johnson, Nan Dai, Andy Hanneman, Ivan R. Corrêa, Jason S. McLellan, et al.mM MgCl2, 2 mM DTT, 0.4 mg ml−1 RNase A (NEB, T3018L) and 0.1 mM final concentration dNTP mix (NEB, N0447L) or an equivalent volume of water were reacted for 15 min at 37 °C, then 2.2 µl of ExoI reaction buffer

(20) Embryo-scale Visual Cell Sorting reveals a conserved transcriptomic signature of nucleolar size linked to proteostasisbioRxivMay 28, 2026Hyeon-Jin Kim, Sriram Pendyala, Josephine Lin, Tony Cooke, Gang Li, William Stafford Noble, Xinxian Deng, Christine M. Disteche, Cole Trapnell, Douglas M. Fowlerwashes, 200 μL of reverse transcription mix (40 μL 5X RT buffer, 8 uL 10mM dNTPs (New England Biolabs, N0447L), 20 μL 40 μM well-specific RT primers, 10 μL RNAseOUT (Thermo Scientific, 10777019), 8 μL Maxima H

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Deoxynucleotide (dNTP) Solution Mix

Deoxynucleotide (dNTP) Solution Mix from New England Biolabs

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New England Biolabs

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Deoxynucleotide (dNTP) Solution Mix from New England Biolabs

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