Fig 1: Expression of IR mRNA splice variants in the human MTG. (A, B) Transcripts of both the short isoform (IR‐A, Ex11−) and the long isoform (IR‐B, Ex11+), probed with RNAscope technology, are shown within the nuclei of NeuN+ neurons by confocal laser scanning at 100× magnification. NeuN is in green, Hoechst nuclear stain is in blue, and RNAscope signal is in red. Scale bar = 10 μm. (C) Density of IR transcript splice‐variants in a tissue microarray of MTG tissue. Normal n = 20; AD n = 18. Data within each disease condition (between isoforms) is paired and compared with a Wilcoxon signed‐rank test. Data within each isoform (between disease conditions) is unpaired and compared with a Mann–Whitney U test. Error bars are ±1 standard deviation. AD, Alzheimer's disease; IR, insulin receptor; MTG, middle temporal gyrus. Other statistical comparison results are presented in Table S7.